GCK家族蛋白激酶MoSOK1调控稻瘟病菌的生长发育与致病性

doi:10.3969/j.issn.1004-1524.2018.06.16

浙江农业学报 ›› 2018, Vol. 30 ›› Issue (6): 999-1007.DOI: 10.3969/j.issn.1004-1524.2018.06.16

GCK家族蛋白激酶MoSOK1调控稻瘟病菌的生长发育与致病性

冯晓晓¹, 李海娇², 李玲^{3, 4}, 王教瑜^{3, *}, 林福呈¹, 卢建平^{2, *}

1.水稻生物学国家重点实验室,浙江大学生物技术研究所,浙江杭州310058;
2.浙江大学生命科学学院,浙江杭州310058;
3.浙江省农业科学院植物保护与微生物研究所,浙江杭州310021;
4.浙江农林大学农业与食品科学学院,浙江杭州 311300

收稿日期:2018-01-05 出版日期:2018-06-20 发布日期:2018-07-02
通讯作者: 卢建平,E-mail: jplu@zju.edu.cn;王教瑜,E-mail: wangjiaoyu78@sina.com
作者简介:冯晓晓(1982—),女,浙江玉环人,硕士,助理实验师,主要从事植物真菌病害致病机理及防控研究。E-mail: xxvon@zju.edu.cn
基金资助:
国家自然科学基金(31170136,31371891,31470249)

MoSOK1, a putative germinal center kinase encoding gene, is required for fungal growth, conidiation and pathogenicity in Magnaporthe oryzae

FENG Xiaoxiao¹, LI Haijiao², LI Ling^{3, 4}, WANG Jiaoyu^{3, *}, LIN Fucheng¹, LU Jianping^{2, *}

1. State Key Laboratory of Rice Biology, Biotechnology Institute, Zhejiang University, Hangzhou 310058, China;
2. College of Life Science, Zhejiang University, Hangzhou 310058, China;
3. Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China;
4. College of Agriculture and Food Science, Zhejiang A and F University, Hangzhou 311300, China

Received:2018-01-05 Online:2018-06-20 Published:2018-07-02

摘要/Abstract

摘要： 稻瘟病菌致病相关基因功能的研究有助于揭示其致病机理,为稻瘟病防治提供理论依据。本研究利用基因替换技术对稻瘟病菌SOK1同源基因(MoSOK1)进行了分析。MoSOK1编码GCK(germinal center kinase)家族的Ste20蛋白激酶,其与全蚀病菌中对应蛋白具有最高同源性。本研究表明,MoSOK1基因在附着胞分化关键期上调表达;与野生型菌株相比,MoSOK1基因缺失突变体菌落颜色加深,气生菌丝减少,生长速度变慢,产孢量下降,分生孢子萌发延迟,致病性降低。另外,交配实验表明,MoSOK1基因缺失突变体能够进行交配,但产子囊壳能力降低。本研究初步表明,MoSOK1基因参与稻瘟病菌生长发育和致病过程。

关键词: 稻瘟病菌, 致病相关基因, MoSOK1

Abstract: SOK1 is a Ste20 protein kinase of the germinal center kinase (GCK) that is activated by oxidant stress and chemical anoxia. It is unknown so far whether SOK1 play roles in regulation of the fungal pathogenicity. Herein, we functionally characterized the SOK1 homologue in the rice blast fungus Magnaporthe oryzae(MoSOK1). MoSOK1 was up-regulated during conidial germination and appressorial formation. Targeted gene replacement showed that MoSOK1 acts as a regulator to fungal development and virulence in M. oryzae. Δmosok1 mutants showed decreased vegetative growth and conidiation, delayed conidial germination and a significant reduction in virulence. MoSOK1 was also found related to the sexual reproduction of the fungus. These data implicate that SOK1 signaling is required for fungal development and pathogenicity in phytopathogenic fungi.

Key words: Magnaporthe oryzae, fungal pathogenicity, MoSOK1

中图分类号:

S435.111.4⁺1

冯晓晓, 李海娇, 李玲, 王教瑜, 林福呈, 卢建平. GCK家族蛋白激酶MoSOK1调控稻瘟病菌的生长发育与致病性[J]. 浙江农业学报, 2018, 30(6): 999-1007.

FENG Xiaoxiao, LI Haijiao, LI Ling, WANG Jiaoyu, LIN Fucheng, LU Jianping. MoSOK1, a putative germinal center kinase encoding gene, is required for fungal growth, conidiation and pathogenicity in Magnaporthe oryzae[J]. , 2018, 30(6): 999-1007.

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GCK家族蛋白激酶MoSOK1调控稻瘟病菌的生长发育与致病性

MoSOK1, a putative germinal center kinase encoding gene, is required for fungal growth, conidiation and pathogenicity in Magnaporthe oryzae

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