虾源蜡样芽孢杆菌D7的生态安全性评价

doi:10.3969/j.issn.1004-1524.2018.09.20

浙江农业学报 ›› 2018, Vol. 30 ›› Issue (9): 1585-1591.DOI: 10.3969/j.issn.1004-1524.2018.09.20

虾源蜡样芽孢杆菌D7的生态安全性评价

张洪玉¹, 王海波^{1, 2}, 杨移斌³, 赵明军⁴, 夏磊^{1, 2, *}

1.中国水产科学研究院无公害渔药创制中心,北京100141;
2.北京鑫洋水产高新技术有限公司,北京102488;
3.中国水产科学研究院长江水产研究所,湖北武汉 430233;
4.中国水产科学研究院渔业发展战略研究中心,北京 100141

收稿日期:2018-03-08 出版日期:2018-09-25 发布日期:2018-10-15
作者简介:张洪玉(1981—),男,内蒙古通辽人,博士,副研究员,主要从事渔用投入品的研究。E-mail: zhanghy@cafs.ac.cn

Ecological safety assessment of Bcillus cereus D7 isolated from shrimp

ZHANG Hongyu¹, WANG Haibo^{1, 2}, YANG Yibin³, ZHAO Mingjun⁴, XIA Lei^{1, 2, *}

1. Research & Creation Center of Green Fishery Drug of Chinese Academy of Fishery Sciences, Beijing 100141, China;
2. Beijing Seasun Aquaculture BioTech. Co. Ltd., Beijing 102488, China;
3. Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China;
4. Fishery Development Strategy & Research Centre,Chinese Academy of Fishery Sciences, Beijing 100141;

Received:2018-03-08 Online:2018-09-25 Published:2018-10-15
Contact: 夏磊,E-mail: xialei666@126.com
Supported by:
中国水产科学研究院基本科研业务费专项(2016B002)

摘要/Abstract

摘要： 为了评价蜡样芽孢杆菌菌株D7的生态安全性,分别测定了蜡样芽孢杆菌D7对氨氮、亚硝酸盐、磷酸盐浓度的影响,以及对小球藻、大型蚤、斑马鱼、凡纳滨对虾和草鱼等水生动植物的安全性。研究结果表明,蜡样芽孢杆菌菌株D7浓度≥1×10⁷ cfu·mL^-1时,水中氨氮及亚硝酸盐含量显著降低(P<0.05)。蜡样芽孢杆菌菌株D7浓度≤1×10⁶ cfu·mL^-1时,对氨氮及亚硝酸盐含量无显著影响。蜡样芽孢杆菌菌株D7浓度在10⁴~10⁸ cfu·mL^-1时,水中磷酸盐含量无显著变化。小球藻含量与菌体浓度呈正相关。处理96 h,对大型蚤、斑马鱼和凡纳滨对虾死亡率均无显著影响。以不同细菌剂量(10⁸~10¹² cfu·mL^-1)灌胃草鱼,处理96 h后,未出现草鱼死亡现象。表明虾源蜡样芽孢杆菌D7生态安全性较好,对水体及养殖动物无明显危害。

关键词: 虾源, 蜡样芽孢杆菌, 生态安全

Abstract: In order to evaluate the ecological safety of Bacillus cereus D7, the effects of Bacillus cereus D7 on concentration of ammonia nitrogen, nitrite and phosphate were tested. The research of safety on Chlorella vulgaris, Daphnia magna, Danio retio, Penaeus vanmamei and Ctenopharyngodon idella were also carried out. The results showed that Bacillus cereus D7 (≥1×10⁷ cfu·mL^-1) reduced ammonia nitrogen and nitrite concentration significantly (P<0.05). When the concentration of Bacillus cereus D7 was below 1×10⁶ cfu·mL^-1, there were no significant influence on ammonia nitrogen and nitrite concentration. When the concentration of Bacillus cereus D7 was between 10⁴~10⁸ cfu·mL^-1, there was no significant influence on phosphate. Concentration of Chlorella vulgaris showed positive correlation with Bacillus cereus D7. The death rate of Daphnia magna, Danio retio,Penaeus Vanmamei had no significant difference after 96 h treatment. When the concentration of Bacillus cereus D7 was between 10⁸~10¹² cfu·mL^-1, there was no obvious influence on death rate of Ctenopharyngodon idella after 96 h. The results showed that Bacillus cereus D7 could have no adverse effects on water and aquatic animals, which could be used as a kind of ecological safety probiotics.

Key words: shrimp source, Bacillus cereus, ecological security

中图分类号:

S966.12

张洪玉, 王海波, 杨移斌, 赵明军, 夏磊. 虾源蜡样芽孢杆菌D7的生态安全性评价[J]. 浙江农业学报, 2018, 30(9): 1585-1591.

ZHANG Hongyu, WANG Haibo, YANG Yibin, ZHAO Mingjun, XIA Lei. Ecological safety assessment of Bcillus cereus D7 isolated from shrimp[J]. , 2018, 30(9): 1585-1591.

参考文献

[1] 刘勇, 张勇, 张和平. 世界益生菌安全性评价方法[J]. 中国食品学报, 2011, 11(6):141-151.
LIU Y, ZHANG Y, ZHANG H P. Evaluating methods of probiotic’s safety in the world[J]. Journal of Chinese Institute of Food Science and Technology , 2011, 11(6): 141-151. (in Chinese with English abstract)
[2] EFSA. Opinion of the Scientific Committee on a request from EFSA on the introduction of a qualified presumption of safety (QPS) approach for assessment of selected microorganisms referred to EFSA[J]. The EFSA Journal , 2017, 587: 1-16.
[3] 中华人民共和国农业部. 微生物肥料生物安全通用技术准则:NY 1109—2006[S]. 北京:中国农业出版社, 2006.
[4] 国家环境保护总局. 环保用微生物菌剂环境安全评价导则:HJ/T 415—2008[S]. 北京:中国环境科学出版社, 2008.
[5] 宋增福, 陈彪, 郭婧,等. 具有群体感应抑制作用的地衣芽孢杆菌T-1的动物安全及生态评价[J]. 水产学报, 2015, 39(9):1395-1404.
SONG Z F, CHEN B, GUO J, et al. Animal safety and ecological evaluation of Bacillus licheniformis T-1 with quorum sensing inhibitory effect[J]. Journal of Fisheries of China , 2015, 39(9): 1395-1404.(in Chinese with English abstract)
[6] 曹海鹏, 卫若鹏, 何珊,等. 水产养殖用解淀粉芽孢杆菌微胶囊的安全性评价[J]. 中国生物工程杂志, 2012, 32(5):58-65.
CAO H P, WEI R P, HE S, et al. Safety evaluation of Bacillus amyloliquefaciens microcapsules used for aquaculture[J]. China Biotechnology , 2012,32(5): 58-65.(in Chinese with English abstract)
[7] 中国兽药典委员会. 中华人民共和国兽药典: 生物制品卷[M]. 北京:中国农业出版社, 2017.
[8] 胡永红, 陈卫, 欧阳平凯. 高效有益微生态制剂开发与利用: 蜡样芽孢杆菌[M]. 北京:化学工业出版社, 2013.
[9] NAVINCHANDRAN M, IYAPPARAJ P, MOOVENDHAN S, et al. Influence of probiotic bacterium Bacillus cereus isolated from the gut of wild shrimp Penaeus monodon in turn as a potent growth promoter and immune enhancer in P. monodon [J]. Fish & Shellfish Immunology , 2014, 36(1):38-45.
[10] 王春迪, 宋晓玲, 张晓静,等. 养殖水体中添加蜡样芽孢杆菌PC465对凡纳滨对虾抗病力的影响[J]. 中国水产科学, 2016, 23(1):146-155.
WANG C D, SONG X L, ZHANG X J, et al. Effects of adding Bacillus cereus PC465 to rearing water on disease resistance of Litopenaeus vannamei [J]. Journal of Fishery Sciences of China , 2016, 23(1):146-155.(in Chinese with English abstract)
[11] 刘文亮, 许华, 唐杨,等. 饲料中补充蜡样芽孢杆菌( Bacillus cereus )生物膜对凡纳滨对虾( Litopenaeus vannamei )生长、抗病力及其肠道微生物组成的影响[J]. 渔业科学进展, 2017, 38(4):87-95.
LIU W L, XU H, TANG Y, et al. Effect of dietary with probiotics on the growth rate, disease resistance and intestinal microflora of Litopenaeus vannamei [J]. Progress in Fishery Sciences , 2017, 38(4):87-95.(in Chinese with English abstract)
[12] HAO K, LIU J Y, LING F, et al. Effects of dietary administration of Shewanella haliotis D4, Bacillus cereus D7 and Aeromonas bivalvium D15, single or combined, on the growth, innate immunity and disease resistance of shrimp, Litopenaeus vannamei [J]. Aquaculture , 2014, 428(3):141-149.
[13] 夏磊, 赵明军, 张洪玉,等. 不同比例复合益生菌对凡纳滨对虾生长、免疫及抗氨氮能力的影响[J]. 中国水产科学, 2015, 22(6):1299-1307.
XIA L, ZHAO M J, ZHANG H Y, et al. Effects of different proportions of a group of compound bacteria on growth, innate immunity, and ammonia nitrogen resistance in Pacific white shrimp, Litopenaeus vannamei [J]. Journal of Fishery Sciences of China , 2015, 22(6):1299-1307.(in Chinese with English abstract)
[14] 谢凤行, 张峰峰, 周可,等. 一株解淀粉芽胞杆菌的分离鉴定及水质净化效果的研究[J]. 环境科学学报, 2012, 32(11):2781-2788.
XIE F X, ZHANG F F, ZHOU K, et al. Identification of a Bacillus amyloliquefaciens strain and its potential application in water purification[J]. Acta Scientiae Circumstantiae , 2012, 32(11):2781-2788.(in Chinese with English abstract)
[15] XIE F, ZHU T, ZHANG F, et al. Using Bacillus amyloliquefaciens for remediation of aquaculture water[J]. Springerplus , 2013, 2(1):119-123.
[16] 中华人民共和国环境保护部. 水质氨氮的测定纳氏试剂分光光度法:HJ 535—2009[S]. 北京: 中国环境科学出版社, 2010.
[17] 中华人民共和国环境保护部. 水质亚硝酸盐氮的测定分光光度法:GB/T 7496—1987[S]. 北京:国家环境保护局, 1987.
[18] 中国国家标准化管理委员会.化学品藻类生长抑制试验:GB/T 21805—2008[S]. 北京: 中国标准出版社, 2008.
[19] 沈萍萍, 王朝晖, 齐雨藻,等. 光密度法测定微藻生物量[J]. 暨南大学学报(自然科学与医学版), 2001, 22(3):115-119.
SHEN P P, WANG Z H, QI Y Z, et al. An optical density method for determination of microalgal biomass[J]. Journal of Jinan University ( Natural Science and medicine edition ), 2001, 22(3):115-119.(in Chinese with English abstract)
[20] 刘湘庆, 姜美洁, 刘璐,等. 浒苔藻体叶绿素提取方法的比较[J]. 海洋环境科学, 2016, 35(1):144-148.
LIU X Q, JIANG M J, LIU L, et al. A comparative study of the different extraction techniques about chlorophyll concentration of Ulva prolifera [J]. Marine Environmental Science , 2016, 35(1):144-148.(in Chinese with English abstract)
[21] ARNON D I. Copper enzymes in isolated chloroplasts polyphenoloxidase in Beta vulgaris [J]. Plant Physiology , 1949, 24(1):1-15.
[22] 国家环境保护局.水质物质对蚤类(大型蚤)急性毒性测定方法:GB/T 13266—1991[S].北京:中国环境科学出版社,1991.
[23] 国家环境保护局.水质物质对淡水鱼(斑马鱼)急性毒性测定方法:GB/T 13267—1991[S].北京:中国环境科学出版社, 1991.
[24] 李卓佳, 周海平, 杨莺莺,等. 乳酸杆菌( Lactobacillus spp. )LH对水产养殖污染物的降解研究[J]. 农业环境科学学报, 2008, 27(1):342-349.
LI Z J, ZHOU H P, YANG Y Y, et al. The degradation of aquiculture contaminants by LH ( Lactobacillus spp. )[J]. Journal of Agro-Environment Science , 2008, 27(1):342-349. (in Chinese with English abstract)
[25] 阎晓静, 王金花, 朱鲁生,等. 卡马西平对小球藻生长的影响和氧化损伤[J]. 农业环境科学学报, 2017, 36(4):643-650.
YAN X J, WANG J H, ZHU L S, et al. Effects of carbamazepine on the growth and the oxidative damage of Chlorella [J]. Journal of Agro-Environment Science , 2017, 36(4):643-650. (in Chinese with English abstract)
[26] 王善龙, 曹煜成, 徐煜,等. 蜡样芽孢杆菌对对虾养殖水体微藻群落的调控研究[J]. 南方水产科学, 2016, 12(1):9-16.
WANG S L, CAO Y C, XU Y, et al. Regulation of Bacillus cereus to microalgae community in shrimp aquaculture water[J], South China Fisheries Science , 2016, 12(1):9-16.(in Chinese with English abstract)
[27] 杨晓凡, 陆光华, 刘建超,等. 环境相关浓度下的药物对大型蚤的多代慢性毒性[J]. 中国环境科学, 2013, 33(3):538-545.
YANG X F, LU G H, LIU J C, et al. Multigenerational chronic effects of pharmaceuticals on Daphnia magna at environmentally relevant concentrations[J]. China Environmental Science , 2013, 33(3):538-545.(in Chinese with English abstract)
[28] SIPES N S, PADILLA S, KNUDSEN T B. Zebrafish: as an integrative model for twenty-first century toxicity testing[J]. Birth Defects Research Part C : Embryo Today Reviews , 2011, 93(3):256-267.

虾源蜡样芽孢杆菌D7的生态安全性评价

Ecological safety assessment of Bcillus cereus D7 isolated from shrimp

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 2

编辑推荐

Metrics

本文评价

[1]	崔一龙, 石芸, 杨达汉, 尹有勤, 薛江东, 霍晓伟, 马德慧. 马源蜡样芽孢杆菌的分离鉴定及毒力基因检测[J]. 浙江农业学报, 2019, 31(2): 216-221.
[2]	尚书;岳德鹏*;曹睿;张亦超;乌尼图. 基于P-S-R模型的北京市朝阳区生态安全评价[J]. , 2013, 25(5): 0-1109.