浙江秀珍菇黄菇病病原菌的分离与鉴定

doi:10.3969/j.issn.1004-1524.2018.11.12

浙江农业学报 ›› 2018, Vol. 30 ›› Issue (11): 1893-1898.DOI: 10.3969/j.issn.1004-1524.2018.11.12

浙江秀珍菇黄菇病病原菌的分离与鉴定

袁卫东¹, 陆娜¹, 宋吉玲¹, 王伟科¹, 亢学平², 闫静¹, 李戌清^1,*

1.杭州市农业科学研究院,浙江杭州 310024;
2.延边朝鲜族自治州农业科学院,吉林龙井 133400

收稿日期:2017-12-25 出版日期:2018-11-25 发布日期:2018-12-21
通讯作者: 李戌清,E-mail:xq_li@126.com
作者简介:袁卫东(1969—),男,浙江杭州人,硕士,高级农艺师,研究方向为食用菌栽培技术研究与示范推广。E-mail: ywd0507@126.com
基金资助:
国家食用菌产业技术体系(CARS-20);杭州市科委科技项目(2015093H06)

Pathogen isolation and identification of yellow rot disease in Pleurotus geesteranus in Zhejiang Province

YUAN Weidong¹, LU Na¹, SONG Jiling¹, WANG Weike¹, KANG Xueping², YAN Jing¹, LI Xuqing^1,*

1. Hangzhou Academy of Agricultural Sciences, Hangzhou 310024, China;
2. Yanbian Korean Autonomous Prefecture Academy of Agricultural Sciences, Longjing 133400, China

Received:2017-12-25 Online:2018-11-25 Published:2018-12-21

摘要/Abstract

摘要： 为明确引起浙江秀珍菇黄菇病的病原菌种类,通过对病原菌的分离纯化、致病性测定、全自动快速微生物质谱检测系统鉴定、脂肪酸甲基酯(FAME)鉴定及16S rRNA序列分析,将引起秀珍菇黄菇病的病原菌鉴定为恶臭假单胞菌Pseudomonas putida。研究结果可为该病害的有效防控提供理论依据。

关键词: 秀珍菇, 黄菇病, 病原菌鉴定, 恶臭假单胞菌

Abstract: In recent years, yellow rot disease occurred severely in Chun'an City, Zhejiang Province, China, and caused serious economic losses in Pleurotus geesteranus production. In order to study the pathogens causing this disease, isolation and purification of pathogens, pathogenicity test, MALDI Biotyper (MBT) test, fatty acid methyl esters (FAME) test and 16S rRNA gene sequence analysis were conducted. The pathogen was identified as Pseudomonas putida. This study could provide scientific data for the effective control of yellow rot disease.

Key words: Pleurotus geesteranus, yellow rot disease, pathogen identification, Pseudomonas putida

中图分类号:

Q939
S432

袁卫东, 陆娜, 宋吉玲, 王伟科, 亢学平, 闫静, 李戌清. 浙江秀珍菇黄菇病病原菌的分离与鉴定[J]. 浙江农业学报, 2018, 30(11): 1893-1898.

YUAN Weidong, LU Na, SONG Jiling, WANG Weike, KANG Xueping, YAN Jing, LI Xuqing. Pathogen isolation and identification of yellow rot disease in Pleurotus geesteranus in Zhejiang Province[J]. , 2018, 30(11): 1893-1898.

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浙江秀珍菇黄菇病病原菌的分离与鉴定

Pathogen isolation and identification of yellow rot disease in Pleurotus geesteranus in Zhejiang Province

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