对猪流行性腹泻病毒灭活疫苗的佐剂作用

doi:10.3969/j.issn.1004-1524.2019.01.07

浙江农业学报 ›› 2019, Vol. 31 ›› Issue (1): 56-61.DOI: 10.3969/j.issn.1004-1524.2019.01.07

对猪流行性腹泻病毒灭活疫苗的佐剂作用

麻勇棋, 徐伟, 王越珉, 胡松华^*

浙江大学动物科学学院,浙江杭州 310058

收稿日期:2018-07-03 出版日期:2019-01-25 发布日期:2019-04-09
通讯作者: 胡松华,E-mail: songhua@zju.edu.cnE515-A
作者简介:麻勇棋(1993—),男,浙江丽水人,硕士研究生,主要从事临床兽医学研究。E-mail: yqmachn1105@163.com
基金资助:
国家自然科学基金(31672598)

Adjuvant effect of E515-A on porcine epidemic diarrhea virus inactivated vaccine

MA Yongqi, XU Wei, WANG Yuemin, HU Songhua^*

College of Animal Sciences, Zhejiang University, Hangzhou 310058, China

Received:2018-07-03 Online:2019-01-25 Published:2019-04-09

摘要/Abstract

摘要： E515-A是一种含人参茎叶皂苷(GSLS)的白油佐剂。将E515-A和含猪流行性腹泻病毒(PEDV)抗原的水相在低剪切力(转速200 r·min^-1)下混合,形成油乳剂疫苗。为观察E515-A对PEDV灭活疫苗的免疫增强作用,将72只ICR小鼠随机分成6组,分别进行2次(间隔2周)皮下注射:(1)0.9% NaCl溶液,(2)PEDV抗原+0.9% NaCl溶液,(3)PEDV抗原+GSLS,(4)PEDV抗原+白油佐剂,(5)PEDV抗原+E515-A和(6)PEDV抗原+氢氧化铝铝胶。于一免后24 h采血检测血清IFN-γ水平和NK细胞杀伤活性;于二免后检测血清特异性IgG及IgG1和IgG2a水平,制备脾淋巴细胞检测细胞增殖能力。结果表明,E515-A对于PEDV疫苗诱导的免疫反应具有显著的促进作用,免疫增强效果优于铝胶佐剂。和对照组比较,E515-A油佐剂可以显著促进免疫小鼠的NK细胞杀伤活性,促进细胞因子IFN-γ的产生,增强脾淋巴细胞对刀豆蛋白A(Con A)和脂多糖(LPS)刺激的增殖应答能力,提高血清IgG及其亚类IgG1和IgG2a水平。为增强猪的疫苗免疫效果,E515-A作为猪疫苗的佐剂值得进一步研究。

关键词: E515-A, 人参茎叶皂甙, 猪流行性腹泻病毒疫苗, 佐剂

Abstract: E515-A was a white oil containing ginseng stem and leaf saponins (GSLS). An oil emulsion vaccine was obtained by mixing E515-A and water phase containing porcine epidemic diarrhea virus (PEDV) antigen at a low shear force (200 r·min^-1). To observe the adjuvant effect of E515-A on inactivated PEDV vaccine, 72 ICR mice were randomly divided into 6 groups and subcutaneously immunized twice at a two-weeks interval with the following solution, respectively: (1) physiological saline solution, (2) PEDV antigen+physiological saline solution, (3) PEDV antigen+GSLS, (4) PEDV antigen+white oil adjuvant, (5) PEDV antigen+E515-A, (6) PEDV antigen+aluminum hydroxide (alum). Serum IFN-γ and NK cytotoxicity were determined 24 h after primary immunization; serum specific IgG, IgG1 and IgG2a and splenic lymphocyte proliferation were analyzed after booster immunization. The results indicated that E515-A significantly enhanced the immune response to PEDV vaccine when compared with aluminum adjuvant. E515-A significantly promoted the killing activity of NK cells in immunized mice, increased the production of IFN-γ, enhanced the splenic lymphocytes proliferation to Concanavalin (Con A) and lipopolysaccharide (LPS) stimulation, and improved the level of serum IgG and its subclass IgG1 and IgG2a in comparison with the control group. To improve the immunity of pigs, E515-A as an adjuvant deserves further study for its use in swine vaccine.

Key words: E515-A, GSLS, PEDV vaccine, adjuvant

中图分类号:

S852.4

麻勇棋, 徐伟, 王越珉, 胡松华. 对猪流行性腹泻病毒灭活疫苗的佐剂作用[J]. 浙江农业学报, 2019, 31(1): 56-61.

MA Yongqi, XU Wei, WANG Yuemin, HU Songhua. Adjuvant effect of E515-A on porcine epidemic diarrhea virus inactivated vaccine[J]. , 2019, 31(1): 56-61.

参考文献

[1] JUNG K, SAIF L J.Porcine epidemic diarrhea virus infection: etiology, epidemiology, pathogenesis and immunoprophylaxis[J]. The Veterinary Journal, 2015, 204(2): 134-143.
[2] 李娇, 王文秀, 谢金文,等. 我国猪流行性腹泻疫苗研究与应用现状[J]. 养猪, 2018 (1):110-112.
LI J, WANG W X, XIE J W, et al.Research and application of porcine epidemic diarrhea vaccine in China[J]. Swine Production, 2018 (1):110-112. (in Chinese)
[3] SONG D, MOON H, KANG B.Porcine epidemic diarrhea: a review of current epidemiology and available vaccines[J]. Clinical and Experimental Vaccine Research, 2015, 4(2): 166-176.
[4] 董世娟, 朱于敏, 于瑞嵩, 等. 我国PEDV分子流行病学研究进展[C]//中国畜牧兽医学会. 中国畜牧兽医学会家畜传染病分会第八次全国会员代表大会暨第15次学术研讨会论文集. 徐州, 2014: 96-101.
[5] 包省军, 宋小明, KEDSIRIN S,等. 人参茎叶皂甙对口蹄疫病毒灭活疫苗免疫后抗体产生的影响[J]. 中国兽医学报, 2009, 29(9):1103-1105.
BAO S J, SONG X M, KEDSIRIN S, et al.Effects of ginsenosides of stems and leaves on immune response to foot and mouth disease attenuated virus vaccine[J]. Chinese Journal of Veterinary Science, 2009, 29(9):1103-1105. (in Chinese with English abstract)
[6] 李禹涛, 燕霞, 谢锋,等. 口蹄疫油乳剂疫苗中添加人参茎叶皂甙对免疫效果和黏滞性的影响[J]. 中国兽医学报, 2013, 33(4):561-565.
LI Y T, YAN X, XIE F, et al.Effects of ginseng stems and leaves saponins (GSLS) on vaccine viscosity and the immune response to foot and mouth disease (FMD) emulsion vaccine[J]. Chinese Journal of Veterinary Science, 2013, 33(4):561-565. (in Chinese with English abstract)
[7] 王明俊, 沈福庆. 矿物油佐剂疫苗研究:Ⅱ、矿物油与乳化剂的选择及乳化方法试验[J]. 中国兽药杂志, 1983 (11):44-48.
WANG M J, SHEN F Q.Study on mineral oil adjuvant vaccine:Ⅱ Selection of mineral oil and emulsifier and test of emulsifying method[J]. Chinese Journal of Veterinary Drug, 1983 (11):44-48. (in Chinese)
[8] LAM V C, LANIER L L.NK cells in host responses to viral infections[J]. Current Opinion in Immunology, 2017, 44:43-51.
[9] LANGEL S N, PAIM F C, LAGER K M, et al.Lactogenic immunity and vaccines for porcine epidemic diarrhea virus (PEDV): historical and current concepts[J]. Virus Research, 2016, 226: 93-107.
[10] SU X, PEI Z, HU S.Ginsenoside Re as an adjuvant to enhance the immune response to the inactivated rabies virus vaccine in mice[J]. International Immunopharmacology, 2014, 20(2): 283-289.
[11] YUAN L, WU L, CHEN J, et al.Paclitaxel acts as an adjuvant to promote both Th1 and Th2 immune responses induced by ovalbumin in mice[J]. Vaccine, 2010, 28(27): 4402-4410.
[12] ZHANG C, WANG Y, WANG M, et al.Rapeseed oil and ginseng saponins work synergistically to enhance Th1 and Th2 immune responses induced by the foot-and-mouth disease vaccine[J]. Clinical and Vaccine Immunology, 2014, 21(8): 1113-1119.
[13] 王萌, 金海波, 张岑容, 等. ZE515 对CP5型金黄色葡萄球菌疫苗的佐剂作用[J]. 中国兽医学报, 2015, 35(8): 1264-1268.
WANG M, JIN H B, ZHANG C R, et al.Efficacy of a Staphylococcus aureus vaccine (CP5 type) adjuvanted with ZE515[J]. Chinese Journal of Veterinary Science, 2015, 35(8): 1264-1268. (in Chinese with English abstract)

对猪流行性腹泻病毒灭活疫苗的佐剂作用

Adjuvant effect of E515-A on porcine epidemic diarrhea virus inactivated vaccine

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