小麦TaeEF1β基因的克隆、同源性及表达分析

doi:10.3969/j.issn.1004-1524.2019.01.13

浙江农业学报 ›› 2019, Vol. 31 ›› Issue (1): 98-103.DOI: 10.3969/j.issn.1004-1524.2019.01.13

小麦TaeEF1β基因的克隆、同源性及表达分析

陈炫¹, 张天烨¹, 羊健², 张恒木², 陈剑平^2,*

1.浙江农林大学林业与生物技术学院,浙江杭州 311300;
2.浙江省农业科学院病毒学与生物技术研究所,浙江杭州 310021

收稿日期:2018-03-25 出版日期:2019-01-25 发布日期:2019-04-09
通讯作者: 陈剑平,E-mail:jpchen2001@126.com
作者简介:陈炫(1992—),女,陕西咸阳人,硕士研究生,主要从事植物病理学研究。E-mail:vivianccx@163.com
基金资助:
国家自然科学基金(3150160); 国家农业产业体系(CARS-3-1); 国家小麦转基因专项(2016ZX08002001)

Gene cloning,homology and expression analysis of TaeEF1β in Triticum aestivum L.

CHEN Xuan¹, ZHANG Tianye¹, YANG Jian², ZHANG Hengmu², CHEN Jianping^2,*

1.College of Forestry and Biotechnology, Zhejiang A&F University, Hangzhou 311300, China;
2.Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:2018-03-25 Online:2019-01-25 Published:2019-04-09

摘要/Abstract

摘要： 真核翻译延伸因子(eukaryotic translation elongation factor,eEFs)是一种重要的多功能调控蛋白,eEF1β是eEF1的组成部分,在蛋白质生物合成过程中发挥着重要的作用。本文通过RT-PCR扩增克隆小麦(Triticum aestivum L.)的eEF1β基因,并命名为TaeEF1β。氨基酸同源性分析发现,TaeEF1β具有高度保守性,且其保守结构域位于137~226 aa处。qRT-PCR结果表明,中国小麦花叶病毒(Chinese wheat mosaic virus,CWMV)侵染小麦植株后,可以诱导TaeEF1β基因转录水平的上调表达。另外,本文也进一步分析了TaeEF1β基因在小麦根、茎、叶的表达水平和CWMV侵染不同时间点的表达情况。

关键词: 真核翻译延伸因子, 中国小麦花叶病毒, 同源性分析

Abstract: Eukaryotic translation elongation factor (eEFs) is an important multifunctional protein. eEF1β was subunit of the eukaryotic translation elongation factor-1 (eEFs-1) complex and played an important role in protein biosynthesis. The eEF1β gene was obtained by cloning with RT-PCR from wheat and named as TaeEF1β. The amino acid sequences of TaeEF1β were highly conserved and the conserved domain was located in 137-226 aa. The results of qRT-PCR showed that TaeEF1β were up-regulated in the CWMV-infected plants. In this study, the expression of TaeEF1β in the stems, leaves and roots of wheat and different stages of CWMV infection was also detected by qRT-PCR.

Key words: eukaryotic translation elongation factor, Chinese wheat mosaic virus, homology analysis

中图分类号:

S435.12

陈炫, 张天烨, 羊健, 张恒木, 陈剑平. 小麦TaeEF1β基因的克隆、同源性及表达分析[J]. 浙江农业学报, 2019, 31(1): 98-103.

CHEN Xuan, ZHANG Tianye, YANG Jian, ZHANG Hengmu, CHEN Jianping. Gene cloning,homology and expression analysis of TaeEF1β in Triticum aestivum L.[J]. , 2019, 31(1): 98-103.

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小麦TaeEF1β基因的克隆、同源性及表达分析

Gene cloning,homology and expression analysis of TaeEF1β in Triticum aestivum L.

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