鸡蛋磺胺间甲氧嘧啶残留研究

doi:10.3969/j.issn.1004-1524.2019.01.20

浙江农业学报 ›› 2019, Vol. 31 ›› Issue (1): 155-160.DOI: 10.3969/j.issn.1004-1524.2019.01.20

鸡蛋磺胺间甲氧嘧啶残留研究

夏魏^1,2, 李祖光¹, 杨华², 吉小凤², 汪建妹², 钱鸣蓉^2,*

1.浙江工业大学化学与工程学院,浙江杭州 310014;
2.浙江省农业科学院农产品质量标准研究所,浙江杭州 310021

收稿日期:2018-03-16 出版日期:2019-01-25 发布日期:2019-04-09
通讯作者: 钱鸣蓉,E-mail: qianmr@mail.zaas.ac.cn
作者简介:夏魏(1993—),男,浙江开化人,硕士研究生,研究方向为分析化学。E-mail: zjutxiawei@sina.com
基金资助:
浙江省特色农产品全产业链安全风险管控(ZJNY2017001); 省部共建国家重点实验室培育基地项目(2010DS700124-ZZ1602); 浙江省农业科学院中美三方合作项目(2010DS700124-ZM1604)

Study on sulfamonomethoxine residue in eggs

XIA Wei^1,2, LI Zuguang¹, YANG Hua², JI Xiaofeng², WANG Jianmei², QIAN Mingrong^2,*

1. College of Chemical Engineering, Zhejiang University of Technology, Hangzhou 310014, China;
2. Institute of Quality and Standard for Agro-Products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:2018-03-16 Online:2019-01-25 Published:2019-04-09

摘要/Abstract

摘要： 随机选择32周龄、体质量1.6 kg的蛋鸡80羽进行试验,在饮水中分别添加159 mg·L^-1(标签推荐最高剂量)和79.5 mg·L^-1的磺胺间甲氧嘧啶,连续用药5 d,分别作为高剂量组和低剂量组,停药后收集鸡蛋样品。鸡蛋样品采用QuEChERS方法进行前处理,采用超高效液相色谱-串联质谱(UPLC-MS/MS)对鸡蛋样品中磺胺间甲氧嘧啶的残留量进行定量测定。结果发现,高剂量组在休药后第1、3、5、10、16、20、24天鸡蛋磺胺间甲氧嘧啶残留量分别为2 497.3、220.1、38.9、6.0、1.3、0.5、0.1 μg·kg^-1,低剂量组在休药后第1、3、5、10、16天鸡蛋磺胺间甲氧嘧啶残留量分别为694.3、45.2、15.5、1.5、0.1 μg·kg^-1。磺胺间甲氧嘧啶在鸡蛋中的消除速率为0.4~0.6 μg·kg^-1·d^-1。高、低剂量组分别在停药后第5天和第3天鸡蛋中磺胺间甲氧嘧啶残留量低于国家限量标准。从食品安全角度考虑,如采用标签推荐最高剂量,建议休药期控制在5 d以上。

关键词: 鸡蛋, 超高效液相色谱串联质谱, 磺胺间甲氧嘧啶, 消除, 休药期

Abstract: Eighty layers of 32-week-old laying hens weighing about 1.6 kg were chosen in this experiment. For test groups, the dose of sulfamonomethoxine was set at 159 mg·L^-1 (label recommended maximum dose) and 79.5 mg·L^-1 in drinking water, respectively. After continuous medication for 5 days, the addition of sulfamonomethoxine in drinking water was stopped and eggs were collected every day. Sulfamonomethoxine residues in egg samples were pretreated with QuEChERS method and quantified by ultra performance liquid chromatography-mass spectrometer/mass spectrometer (UPLC-MS/MS). The results showed that the residual amount of sulfamonomethoxine in 159 mg·L^-1 test group was 2 497.3, 220.1, 38.9, 6.0, 1.3, 0.5, 0.1 μg·kg^-1 on the 1^st, 3^rd, 5^th, 10^th, 16^th, 20^th, 24^th day, respectively. The residues of sulfamonomethoxine in 79.5 mg·L^-1 test group were 694.3, 45.2, 15.5, 1.5, 0.1 μg·kg^-1 on the 1^st, 3^rd, 5^th, 10^th, 16^th day, respectively. Sulfamonomethoxine depletion rate in eggs was 0.4-0.6 μg·kg^-1·d^-1. According to the maximum residue limit (MRL) of sulfamonomethoxine in animal tissue set by China, the residual amount in eggs was lower than MRL on 5^th and 3^rd day in 159 mg·L^-1 and 79.5 mg·L^-1 test group, respectively, without drug application. To guarantee food safety, the proposed withdrawal period should be set at more than 5 days if the label recommended maximum dose of sulfamonomethoxine was applied.

Key words: egg, ultra performance liquid chromatography-mass spectrometer/mass spectrometer, sulfamonomethoxine, elimination, withdrawal period

中图分类号:

S859.84

夏魏, 李祖光, 杨华, 吉小凤, 汪建妹, 钱鸣蓉. 鸡蛋磺胺间甲氧嘧啶残留研究[J]. 浙江农业学报, 2019, 31(1): 155-160.

XIA Wei, LI Zuguang, YANG Hua, JI Xiaofeng, WANG Jianmei, QIAN Mingrong. Study on sulfamonomethoxine residue in eggs[J]. , 2019, 31(1): 155-160.

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鸡蛋磺胺间甲氧嘧啶残留研究

Study on sulfamonomethoxine residue in eggs

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