苗药小花清风藤叶绿体基因psbA-trnH序列特征及遗传多样性分析

doi:10.3969/j.issn.1004-1524.2020.05.08

浙江农业学报 ›› 2020, Vol. 32 ›› Issue (5): 810-815.DOI: 10.3969/j.issn.1004-1524.2020.05.08

苗药小花清风藤叶绿体基因psbA-trnH序列特征及遗传多样性分析

严福林, 王波, 温迪, 徐文芬, 孙庆文^*, 魏升华

贵州中医药大学药学院,贵州贵阳 550025

收稿日期:2019-11-14 出版日期:2020-05-25 发布日期:2020-05-29
通讯作者: *,孙庆文,E-mail:1016923718@qq.com
作者简介:严福林(1986—),男,贵州安龙人,硕士,讲师,主要从事中药与民族药资源研究。E-mail:flyan00@qq.com
基金资助:
国家自然科学基金(81560707); 贵州省国内一流学科建设项目(GNYL〔2017〕008); 贵州省普通高等学校工程研究中心项目(黔教合KY字〔2017〕018); 贵州省优秀青年科技人才项目(黔科合平台人才〔2019〕5658); 国家中医药管理局资助项目(国中医药科技中药2014-117号)

Genetic diversity analysis of Sabia parviflora based on chloroplast gene psbA-trnH sequence

YAN Fulin, WANG Bo, WEN Di, XU Wenfen, SUN Qingwen^*, WEI Shenghua

Department of Pharmacy, Guizhou University of Traditional Chinese Medicine, Guiyang 550025, China

Received:2019-11-14 Online:2020-05-25 Published:2020-05-29

摘要/Abstract

摘要： 基于psbA-trnH序列分析,探讨黔产小花清风藤种质资源遗传多样性、系统进化与分子鉴定方法,对贵州苗药小花清风藤4个县区产地13个居群样品进行psbA-trnH序列PCR扩增。应用Codon Code Aligner 8.0.2软件进行校对拼接,分析居群遗传距离、单倍型数量与多样性,构建系统进化树。结果表明:13条小花清风藤叶绿体基因psbA-trnH 序列长度为469~488 bp,多态性位点数共37个,简约信息位点14个,自裔位点23个,插入/缺失片段3个;居群间的遗传距离变化范围为0~0.085 9,平均遗传距离为0.025 3,具有4个单倍型,单倍型(基因)多样性(Hd)为0.679;Fu and Li's D^*检验、Fu and Li's F^*检验、Tajima's D检验中P值均大于0.10,无统计学意义,说明黔产小花清风藤进化速率不一致,遗传多样性分化较丰富。本研究丰富了小花清风藤及其同属植物的研究数据,为小花清风藤的分类鉴定、系统演化和分子标记开发等研究提供了一定参考。

关键词: 小花清风藤, psbA-trnH, 贵州, 遗传多样性

Abstract: System evolution relationship, genetic diversity and molecular identification method of the germplasm resources of Sabia parviflora from different regions of Guizhou were analyzed based on the sequence of psbA-trnH chloroplast gene. The psbA-trnH sequences of 13 Sabia parviflora samples from 4 counties and districts in Guizhou were amplified and sequenced by PCR. Codon Code Aligner 8.0.2 software was applied for collation and splicing, and population genetic distance, haplotype number and diversity were analyzed to construct phylogenetic tree. The length of psbA-trnH sequence of 13 chloroplast genes of Sabia parviflora was 469-488 bp, with 37 polymorphism sites, 14 reduced information sites, 23 indigenous sites, and 3 inserted/deleted fragments. The variation range of genetic distance between different populations was 0-0.085 9, and the average genetic distance was 0.025 3, with 4 haplotypes, haplotype (gene) diversity (Hd) of 0.679. In Fu and Li's D^* test, Fu and Li's F^* test, and Tajima's D test, P values were all greater than 0.10, without statistical significance. In conclusion, the evolution rate of Sabia parviflora was not consistent, and the genetic diversity was rich. This study enriched the research data of Sabia parviflora and its genera, and provided a certain reference for the classification, identification, systematic evolution and molecular marker development of Sabia parviflora.

Key words: Sabia parviflora, psbA-trnH sequence, Guizhou, genetic diversity

中图分类号:

S567

严福林, 王波, 温迪, 徐文芬, 孙庆文, 魏升华. 苗药小花清风藤叶绿体基因psbA-trnH序列特征及遗传多样性分析[J]. 浙江农业学报, 2020, 32(5): 810-815.

YAN Fulin, WANG Bo, WEN Di, XU Wenfen, SUN Qingwen, WEI Shenghua. Genetic diversity analysis of Sabia parviflora based on chloroplast gene psbA-trnH sequence[J]. , 2020, 32(5): 810-815.

参考文献

[1] 吴征镒,洪德元.Flora of China:第12卷[M].北京:科学出版社, 2009.
[2] 张雪晴, 李曼姝, 李玉玲, 等. 我国清风藤属药用植物研究概况[J]. 生物资源, 2018, 40(6): 477-490.
ZHANG X Q, LI M S, LI Y L, et al.Research progress on Sabia medicinal plants in China[J]. Biotic Resources, 2018, 40(6): 477-490.(in Chinese with English abstract)
[3] 贵州省药品监督管理局. 贵州省中药材、民族药材质量标准[S].贵阳:贵州科技出版社,2003.
[4] 何顺志, 徐文芬. 贵州中草药资源研究[M]. 贵阳: 贵州科技出版社, 2007.
[5] 陈谨, 邓赟, 唐天君, 等. 小花清风藤三萜成分的研究[J]. 中草药, 2004, 35(1): 16-17.
CHEN J, DENG Y, TANG T J, et al.Study on the constituents of triterpenoids of Sabia parviflora[J]. Chinese Traditional and Herbal Drugs, 2004, 35(1): 16-17.(in Chinese)
[6] 赵兰君, 王玉伟, 李志峰, 等. 小花清风藤化学成分的分离与鉴定[J]. 中草药, 2018, 49(3): 544-548.
ZHAO L J, WANG Y W, LI Z F, et al.Isolation and identification of chemical constituents from Sabia parviflora[J]. Chinese Traditional and Herbal Drugs, 2018, 49(3): 544-548.(in Chinese with English abstract)
[7] 李曼姝, 李建桥, 胡建忠, 等. 小花清风藤的化学成分及其对肝细胞的保护作用[J]. 生物资源, 2018, 40(6): 491-494.
LI M S, LI J Q, HU J Z, et al.Chemical constituents of Sabia parviflora and their hepatocyte protection activity[J]. Biotic Resources, 2018, 40(6): 491-494.(in Chinese with English abstract)
[8] 杨莹, 张永萍, 梁光义. 小花清风藤胶囊的保肝作用研究及其急毒实验[J]. 中国现代应用药学, 2013, 30(11): 1166-1170.
YANG Y, ZHANG Y P, LIANG G Y.Protective effect of Xiaohuaqingfengteng capsule on acute liver injury mice and its acute toxicity test[J]. Chinese Journal of Modern Applied Pharmacy, 2013, 30(11): 1166-1170.(in Chinese with English abstract)
[9] FUNABASHI M. Augmentation of plant genetic diversity in synecoculture: theory and practice in temperate and tropical zones[M]//Sustainable Development and Biodiversity. Cham: Springer International Publishing, 2019: 3-46.
[10] SHI X W, GONG C, ZHANG L, et al.Which species should we focus on? umbrella species assessment in southwest China[J]. Biology, 2019, 8(2): 42.
[11] 高连明. DNA条形码在生物多样性编目与评价中的应用[J]. 生物多样性, 2015, 23(3): 286-287.
GAO L M.Applications of DNA barcoding in biodiversity inventory and assessment[J]. Biodiversity Science, 2015, 23(3): 286-287.(in Chinese)
[12] INTHARUKSA A, SASAKI Y, ANDO H, et al.The combination of ITS2 and psbA-trnH region is powerful DNA barcode markers for authentication of medicinal Terminalia plants from Thailand[J]. Journal of Natural Medicines, 2020, 74(1): 282-293.
[13] 赵海, 李园园, 刘红昌, 等. 基于ITS2和psbA-trnH序列的黔太子参品种DNA条形码鉴定研究[J]. 中药材, 2018, 41(4): 855-858.
ZHAO H, LI Y Y, LIU H C, et al.DNA barcoding identification of Pseudostellaria heterophylla based on ITS2 and psba-trnh sequences[J]. Journal of Chinese Medicinal Materials, 2018, 41(4): 855-858.(in Chinese)
[14] 张宏意, 袁林林, 饶秋红, 等. 不同产地何首乌叶绿体psbA-trnH基因序列分析[J]. 中草药, 2018, 49(5): 1146-1149.
ZHANG H Y, YUAN L L, RAO Q H, et al.Sequence analysis of psbA-trnH gene in chloroplast of Polygonum multiflorum from different producing areas[J]. Chinese Traditional and Herbal Drugs, 2018, 49(5): 1146-1149.(in Chinese with English abstract)
[15] 林爽, 吴海燕, 张宏意, 等. 不同地理居群破布叶的psbA-trnH和ITS2序列及其聚类分析[J]. 中草药, 2017, 48(7): 1403-1408.
LIN S, WU H Y, ZHANG H Y, et al.Cluster analysis on psbA-trnH and ITS2 sequences of Microcos paniculata from different geographical populations[J]. Chinese Traditional and Herbal Drugs, 2017, 48(7): 1403-1408.(in Chinese with English abstract)
[16] KUMAR S, STECHER G, TAMURA K.MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets[J]. Molecular Biology and Evolution, 2016, 33(7): 1870-1874.
[17] ROZAS J, SANCHEZ-DELBARRIO J C, MESSEGUER X, et al. DnaSP, DNA polymorphism analyses by the coalescent and other methods[J]. Bioinformatics, 2003, 19(18): 2496-2497.

苗药小花清风藤叶绿体基因psbA-trnH序列特征及遗传多样性分析

Genetic diversity analysis of Sabia parviflora based on chloroplast gene psbA-trnH sequence

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	洪霞, 赵永彬, 屈为栋, 陈银龙, 邱莉萍, 王娇阳. 基于表型性状与简单重复序列标记的浙江省芋种质资源遗传多样性比较[J]. 浙江农业学报, 2020, 32(9): 1544-1554.
[2]	牛素贞, 安红卫, 宋勤飞, 陈正武. 贵州野生茶树立地土壤养分状况分析及综合评价[J]. 浙江农业学报, 2020, 32(6): 1039-1048.
[3]	刘春林, 张建, 彭益书, 倪莘然, 杨瑞东. 贵州雷山茶区土壤-茶叶重金属含量特征及饮茶风险评价[J]. 浙江农业学报, 2020, 32(6): 1049-1059.
[4]	郭勤卫, 张婷, 刘慧琴, 章心惠, 李朝森, 项小敏, 赵东风, 万红建. 应用ISSR分子标记评价我国丝瓜种质资源遗传多样性[J]. 浙江农业学报, 2020, 32(4): 616-623.
[5]	张波, 于飞, 吴战平, 胡家敏. 贵州霜冻气候变化特征[J]. 浙江农业学报, 2020, 32(4): 685-695.
[6]	胡倩文, 徐延浩, 王容, 张文英, 华为, 吕超. 大麦4个穗部性状的关联分析[J]. 浙江农业学报, 2020, 32(11): 1941-1953.
[7]	谢文钢, 李晓松, 李伟, 唐茜. 四川地方中小叶茶树资源的表型遗传多样性[J]. 浙江农业学报, 2019, 31(9): 1405-1415.
[8]	尹潇潇, 李燕方, 古江, 廖艳, 谢跃, 杨光友, 古小彬. 基于线粒体ATP6基因全序列分析中国绵羊痒螨(兔亚种)的遗传多样性[J]. 浙江农业学报, 2019, 31(8): 1231-1238.
[9]	唐首杰, 毕详, 张飞明, 张友良. 连续三代减数分裂雌核发育团头鲂的遗传多样性分析和RAPD鉴别方法的建立[J]. 浙江农业学报, 2019, 31(8): 1257-1271.
[10]	岳高红, 潘彬荣, 刘永安, 梅喜雪, 许立奎, 张宗宸, 周志辉. 利用SSR分析浙南地区甜玉米自交系的遗传多样性[J]. 浙江农业学报, 2019, 31(7): 1029-1036.
[11]	姜武, 吴时武, 吴志刚, 陶正明. 基于SRAP分子标记技术研究浙产栀子遗传多样性[J]. 浙江农业学报, 2019, 31(3): 408-413.
[12]	王伟科, 袁卫东, 陆娜, 宋吉玲, 闫静. 基于ISSR的野生桑树桑黄菌株遗传多样性分析[J]. 浙江农业学报, 2019, 31(3): 414-419.
[13]	张波, 谷晓平, 古书鸿, 胡家敏. 贵州水稻有效降水量和需水量特征分析[J]. 浙江农业学报, 2019, 31(2): 191-199.
[14]	王伟科, 宋吉玲, 巫优良, 闫静, 陆娜, 袁卫东, 陈观平. 药用真菌桑黄分子鉴定及遗传多样性分析[J]. 浙江农业学报, 2019, 31(2): 307-314.
[15]	赵文杰, 徐薇, 张景龙, 程爽, 郑甲成, 刘言龙, 殷世霞, 李杰勤, 詹秋文. 甜高粱生物学性状与SSR分子标记遗传多样性[J]. 浙江农业学报, 2019, 31(12): 1945-1954.