Effects of melatonin seed priming on growth and physiological characteristics of Capsicum annuum under drought stress

doi:10.3969/j.issn.1004-1524.20221227

Acta Agriculturae Zhejiangensis ›› 2023, Vol. 35 ›› Issue (10): 2378-2388.DOI: 10.3969/j.issn.1004-1524.20221227

• Horticultural Science • Previous Articles Next Articles

Effects of melatonin seed priming on growth and physiological characteristics of Capsicum annuum under drought stress

WEI Xiya(), LIANG Lamei, LIN Xinqi, QIN Zhongwei, LI Yingzhi()

Binhai Agricultural College of Guangdong Ocean University, Zhanjiang 524000, Guangdong, China

Received:2022-08-21 Online:2023-10-25 Published:2023-10-31

Abstract

Abstract:

In order to explore the regulatory effect of melatonin seed priming treatment on drought tolerance of pepper, the effects of 10 different concentrations of melatonin seed priming treatment on plant growth and physiological characteristics of pepper were analyzed by pot PEG simulating drought stress with Maoshu 360 as the material. The results showed that the root length, plant height, root-shoot ratio, root fresh weight, shoot fresh weight, shoot dry weight and root dry weight increased firstly and then decreased with the increase of starter solution concentration, and the plant height, shoot fresh weight and shoot dry weight reached the maximum when the concentration was 100 μmol·L^-1. The root length, root shoot ratio, root fresh weight and root dry weight reached the maximum when the concentration was 75 μmol·L^-1. Physiological analysis showed that the contents of malondialdehyde, hydrogen peroxide and superoxide anion were the lowest, while the contents of soluble sugar, soluble protein, proline, AsA, DHA and antioxidant enzyme activities (POD, SOD, APX and GR) were the highest in leaves under drought stress induced by 100 μmol·L^-1 melatonin. After 75 μmol·L^-1 melatonin, the contents of malondialdehyde, hydrogen peroxide, and superoxide anion were the lowest, and the soluble sugar, soluble protein, proline contents, antioxidant enzymes (POD, SOD, CAT, APX and GR) activities, AsA, DHA contents and AsA/DHA in roots were the highest. The activity of CAT and contents of soluble protein and AsA/DHA in leaves were the highest. These results indicated that 100 μmol·L^-1 and 75 μmol·L^-1 melatonin seed priming treatment was beneficial to improve drought resistance of pepper.

Key words: drought stress, pepper, melatonin, leaf, root

CLC Number:

S641.3

WEI Xiya, LIANG Lamei, LIN Xinqi, QIN Zhongwei, LI Yingzhi. Effects of melatonin seed priming on growth and physiological characteristics of Capsicum annuum under drought stress[J]. Acta Agriculturae Zhejiangensis, 2023, 35(10): 2378-2388.

Figures/Tables 7

Table 1 Effects of seed priming treatments with different concentrations of melatonin on plant height, root length and root-shoot ratio of pepper under drought stress

处理Treatment	株高Plant height/cm	根长Root length/cm	根冠比Root-shoot ratio
CK	11.034±0.061 e	12.687±0.483 g	1.150±0.043 f
T0	11.829±0.158 d	15.679±0.774 f	1.328±0.083 e
T1	12.260±0.192 cd	20.232±0.430 e	1.652±0.058 bcd
T5	12.629±0.302 bc	19.983±0.218 e	1.583±0.024 cd
T25	12.954±0.325 bc	22.902±0.700 cd	1.770±0.072 ab
T50	13.349±0.290 b	23.180±0.875 bc	1.736±0.048 abc
T75	14.486±0.362 a	26.794±0.433 a	1.852±0.055 a
T100	15.042±0.164 a	24.867±0.148 b	1.653±0.009 bcd
T125	12.718±0.346 bc	21.211±0.669 de	1.668±0.012 bcd
T150	10.838±0.101 e	16.381±0.772 f	1.512±0.077 d

Table 2 Effects of melatonin seed priming treatments with different concentrations on fresh weight and dry weight of root and shoot of pepper under drought stress g

处理 Treatment	根鲜重 Root fresh weight	地上部鲜重 Shoot fresh weight	根干重 Root dry weight	地上部干重 Shoot dry weight
CK	1.359±0.053 e	1.583±0.055 fg	0.072±0.005 f	0.115±0.006 f
T0	1.552±0.04 d	1.766±0.081 e	0.102±0.005 de	0.216±0.007 e
T1	1.596±0.035 d	1.731±0.005 ef	0.114±0.007 d	0.206±0.008 e
T5	1.574±0.048 d	1.720±0.050 ef	0.134±0.005 c	0.241±0.015 e
T25	1.932±0.041 c	2.286±0.056 d	0.145±0.004 c	0.290±0.015 d
T50	2.101±0.052 b	2.31±0.048 cd	0.167±0.003 b	0.323±0.007 cd
T75	2.401±0.035 a	2.455±0.079 bc	0.193±0.007 a	0.471±0.014 b
T100	2.313±0.029 a	2.969±0.015 a	0.185±0.007 a	0.509±0.020 a
T125	1.978±0.060 bc	2.549±0.014 b	0.166±0.003 b	0.462±0.011 b
T150	1.065±0.037 f	1.484±0.080 g	0.097±0.006 e	0.355±0.013 c

Fig.1 Effects of seed priming treatments with different concentrations of melatonin on MDA (A), hydrogen peroxide (B) and superoxide anion (C) contents in leaves and roots of pepper under drought stress The treatments of the same tissue with different lowercase letters show the significant difference(P<0.05). The same as below.

Fig.2 Effects of seed priming with different concentrations of melatonin on the contents of soluble protein (A), soluble sugar (B) and proline (C) in leaves and roots of pepper under drought stress

Fig.3 Effects of seed priming with different concentrations of melatonin on POD (A), SOD (B) and CAT (C) activities in leaves and roots of pepper under drought stress

Fig.4 Effects of seed priming treatments with different concentrations of melatonin on APX (A) and GR (B) activities in roots and leaves of pepper under drought stress

Fig.5 Effects of seed priming treatments with different concentrations of melatonin on contents of AsA (A), DHA (B) and AsA/DHA(C) in leaves and roots of pepper under drought stress

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Effects of melatonin seed priming on growth and physiological characteristics of Capsicum annuum under drought stress

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