SSR荧光标记毛细管电泳法分析30份无花果品种的遗传多样性

doi:10.3969/j.issn.1004-1524.2017.09.09

浙江农业学报 ›› 2017, Vol. 29 ›› Issue (9): 1482-1488.DOI: 10.3969/j.issn.1004-1524.2017.09.09

SSR荧光标记毛细管电泳法分析30份无花果品种的遗传多样性

郭正兵¹, 韩柏明¹, 郭强²

1.江苏农林职业技术学院,江苏句容212400;
2.句容虎耳山无花果专业合作社,江苏句容212400

收稿日期:2017-04-20 出版日期:2017-09-20 发布日期:2017-09-27
作者简介:郭正兵(1976—),男,江苏高邮人,硕士,副教授,主要从事葡萄、草莓、无花果等果树栽培生理、品源亲缘鉴定等研究。E-mail:260626813@qq.com
基金资助:
江苏农林职业技术学院院级项目(2015KJ030); 江苏高校品牌专业建设工程资助项目(PPZY2015B173)

Genetic diversity analysis of 30 fig varieties using capillary electrophoresis detection with fluorescent SSR markers

GUO Zhengbing¹, HAN Baiming¹, GUO Qiang²

1.Jiangsu Polytechnical College of Agriculture and Forestry, Jurong 212400, China;
2. Jurong Tigerhill Fig Professional Cooperatives, Jurong 212400, China

Received:2017-04-20 Online:2017-09-20 Published:2017-09-27

摘要/Abstract

摘要： 为了分析从国内外搜集的无花果品种的遗传多样性,利用SSR荧光标记毛细管电泳检测法构建了30份无花果品种的指纹鉴定平台。以15对SSR荧光引物对30份无花果品种资源进行分析,结果共检测到79个等位变异。15个位点的PIC值在0.332 8~0.875 7,平均为0.530 3;各位点的 Shannon 信息指数(I) 为0.604 9~2.127 3,平均为 1.136 0。30份品种资源的遗传距离在0.025 4~1.123 4。其中引物FCUP038-6的杂合度、PIC 值分别高达0.846 8、0.861 1,可考虑作为以后区试杂交种纯度鉴定的核心标记。30份无花果品种资源表现出比较丰富的遗传多样性,聚类分析结果显示,30份资源被分为两组,来源不同的品种被分散聚类。同时,无花果品种的亲缘关系与其来源和表型特征并不存在明显的相关性。

关键词: 无花果, 遗传多样性, SSR

Abstract: In order to analyze the genetic diversity of the fig species collected from home and abroad, the capillary electrophoresis detection with fluorescent SSR markers method was used to establish the fingerprint identification platform of 30 fig varieties. Analysis of 30 fig varieties was performed by using 15 pairs of SSR primers. The results showed that a total of 79 alleles were detected, the PIC value of 15 loci was between 0.332 8 and 0.875 7 with an average of 0.530 3 and the Shannon information index (I) of each point was between 0.604 9 to 2.127 3 with an average of 1.136 0. The genetic distance between the 30 varieties was 0.025 4-1.123 4. Among them, the heterozygosity and PIC value of primer FCUP038-6 were up to 0.846 8 and 0.861 1, respectively, which could be considered as the core marker of the purity of the hybrid test. Thirty fig varieties were rich in genetic diversity and were divided into two groups with different origins. There was no significant correlation between the genetic relationships and the origins of the varieties, as well as the phenotype feature.

Key words: Ficus carica L, genetic diversity, SSR

中图分类号:

S663.3

郭正兵, 韩柏明, 郭强. SSR荧光标记毛细管电泳法分析30份无花果品种的遗传多样性[J]. 浙江农业学报, 2017, 29(9): 1482-1488.

GUO Zhengbing, HAN Baiming, GUO Qiang. Genetic diversity analysis of 30 fig varieties using capillary electrophoresis detection with fluorescent SSR markers[J]. , 2017, 29(9): 1482-1488.

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SSR荧光标记毛细管电泳法分析30份无花果品种的遗传多样性

Genetic diversity analysis of 30 fig varieties using capillary electrophoresis detection with fluorescent SSR markers

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