肝过表达hNPC1L1促进脂质过氧化

doi:10.3969/j.issn.1004-1524.2017.10.09

浙江农业学报 ›› 2017, Vol. 29 ›› Issue (10): 1654-1660.DOI: 10.3969/j.issn.1004-1524.2017.10.09

肝过表达hNPC1L1促进脂质过氧化

许崇利¹, 许崇波², 宫语晨³, 欧阳红生^{4, *}

1.吉林化工学院生物与食品工程学院,吉林吉林132022;
2.韶关学院英东生命科学院,粤北生猪生产及疫病防控协同创新发展中心,广东韶关512005;
3.吉林农业大学动物科学技术学院,吉林长春130118;
4.吉林大学动物医学学院,吉林长春 130062

收稿日期:2016-10-12 出版日期:2017-10-20 发布日期:2017-12-05
通讯作者: 欧阳红生,E-mail:ouyh@jlu.edu.cn
作者简介:许崇利(1974-),男,黑龙江鸡东人,博士,教授,研究方向为微生物分子生物学。E-mail: xcl902@163.com
基金资助:
国家科技支撑计划(2011BA115B02)

Overexpression of human Niemann-Pick C1-Like 1 in livers accelerates lipid peroxidation

XU Chongli¹, XU Chongbo², GONG Yuchen³, OUYANG Hongsheng^{4, *}

1. College of Biology and Food Engineering, Jilin Institute of Chemical Technology, Jilin 132022, China;
2. North Guangdong Collaborative Innovation and Development Center for Swine Farming and Disease Control, Yingdong College of Life Sciences, Shaoguan University, Shaoguan 512005, China;
3. College of Animal Science, Jilin Agricultural University, Changchun 130118, China;
4. College of Veterinary Medicine, Jilin University, Changchun 130062, China

Received:2016-10-12 Online:2017-10-20 Published:2017-12-05

摘要/Abstract

摘要： 利用体细胞核移植技术将构建的肝过表达hNPC1L1的小型猪胎儿成纤维细胞作为核供体细胞,构建了肝过表达hNPC1L1转基因巴马小型猪,PCR和RT-PCR鉴定表明hNPC1L1特异性地表达于肝组织。对转基因巴马小型猪进行了初步研究,脂质过氧化标记物丙二醛(MDA)检测表明:转基因猪MDA含量(7.51±0.09)nmol·mL^-1显著高于非转基因猪(3.56±0.16)nmol·mL^-1(P<0.001),说明肝组织发生了严重的脂质过氧化。超氧化物歧化酶(SOD)活性检测表明:SOD活性为(131±4.61)U·mg^-1显著低于非转基因猪[(229.2±4.39)U·mg^-1](P<0.001),说明肝脏清除自由基和抗氧化能力大大下降。试验揭示出在肝脏过表达hNPC1L1导致严重的脂质过氧化,表明NPC1L1在脂代谢紊乱和肝脏疾病中扮演着重要的角色,有望成为肝脏疾病治疗新的靶标。

关键词: hNPC1L1, 过表达, 体细胞核移植, 脂质过氧化

Abstract: The transgenic Bama miniature pigs in which human Niemann-Pick C1-Like 1(hNPC1L1) was overexpressed in the livers were constructed by using somatic cell nuclear transfer technique with the minipig fetal fibroblast cells as the nuclear donor cells. The hNPC1L1 was specifically expressed in transgenic liver tissues as revealed by PCR and reverse transcriptase (RT)-PCR analysis. A preliminary study on transgenic Bama miniature pigs was carried out, and the marker malondialdehyde (MDA) of lipid peroxidation was detected. The result showed that the level of MDA in transgenic pigs [(7.51±0.09) nmol·mL^-1] was significantly higher than that in non-transgenic pigs [(3.56±0.16) nmol·mL^-1] (P<0.001), but SOD level in transgenic pigs [(131±4.61)U·mg^-1] was significantly lower than that in non-transgenic pigs [(229.2±4.39)U·mg^-1] (P<0.001). All of these suggested that severe lipid peroxidation occurred in the liver and the oxygen free radical scavenging and antioxidant capacity were significantly reduced. This study revealed that overexpression of NPC1L1 in the liver led to severe lipid peroxidation, which indicated that NPC1L1 played an important role in lipid metabolism disorders and liver diseases and could be a new target for liver disease treatment.

Key words: human Niemann-Pick C1-Like 1, overexpression, somatic cell nuclear transfer, lipid peroxidation

中图分类号:

S828

许崇利, 许崇波, 宫语晨, 欧阳红生. 肝过表达hNPC1L1促进脂质过氧化[J]. 浙江农业学报, 2017, 29(10): 1654-1660.

XU Chongli, XU Chongbo, GONG Yuchen, OUYANG Hongsheng. Overexpression of human Niemann-Pick C1-Like 1 in livers accelerates lipid peroxidation[J]. , 2017, 29(10): 1654-1660.

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肝过表达hNPC1L1促进脂质过氧化

Overexpression of human Niemann-Pick C1-Like 1 in livers accelerates lipid peroxidation

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