江西铅山红芽芋和青秆芋的转录组比较分析

doi:10.3969/j.issn.1004-1524.2020.09.02

浙江农业学报 ›› 2020, Vol. 32 ›› Issue (9): 1533-1543.DOI: 10.3969/j.issn.1004-1524.2020.09.02

江西铅山红芽芋和青秆芋的转录组比较分析

尹明华, 曹晴, 陈红, 邓思宇, 邓燕梅

上饶师范学院生命科学学院,江西上饶 334001

收稿日期:2020-02-15 出版日期:2020-09-25 发布日期:2020-10-10
作者简介:尹明华(1973—),女,江西永新人,硕士,副教授,主要从事植物生物技术方面的研究。E-mail:yinminghua04@163.com
基金资助:
国家自然科学基金(31860084)

Transcriptome analysis of red bud taro and green stem taro in Yanshan, Jiangxi Province

YIN Minghua, CAO Qing, CHEN Hong, DENG Siyu, DENG Yanmei

College of Life Sciences, Shangrao Normal University, Shangrao 334001, China

Received:2020-02-15 Online:2020-09-25 Published:2020-10-10

摘要/Abstract

摘要： 为探究江西铅山红芽芋和江西铅山青秆芋之间的分子差异,以江西铅山红芽芋(HYY组)和江西铅山青秆芋(QGY组)的试管苗为试验材料进行转录组分析。结果表明:HYY组Clean reads为41 298 676,GC含量为53.09%;QGY组Clean reads为45 551 860,GC含量为51.17%。HYY组和QGY组表达量FPKM的对数值在0~2,表达量密度在0~0.7。HYY组和QGY组表达的共有基因数为14 038,HYY组单独表达的基因数为17 843,QGY组单独表达的基因数为18 634。HYY组和QGY组表达量的相关系数为0.035,样本间相关性较差。HYY组和QGY组共产生差异表达基因32 555个,与QGY组比较,HYY组上调基因15 887,下调基因16 668。GO 富集分析显示,差异基因主要注释到多糖代谢过程、基因沉默、果胶分解代谢过程、碳水化合物代谢过程、生长素激活的信号通路、细胞外区、膜的固有成分、膜的组成成分、膜部件、凋亡细胞、作用于糖基键水解酶活性、水解O-糖基化合物的水解酶活性、蛋白质二聚活性、DNA结合、氧化还原酶活性对二酚及其相关物质的作用等功能。KEGG富集分析显示,差异基因主要注释到植物激素信号转导、植物病原相互作用、半胱氨酸与蛋氨酸代谢、苯丙酸生物合成、皮质醇合成与分泌、过氧化物酶体、植物MAPK信号途径、不匹配修复、淀粉和蔗糖代谢、硫代谢、戊糖和葡萄糖醛酸相互转化、C型凝集素受体信号通路、甘油酯代谢、不饱和脂肪酸的生物合成、α-亚麻酸代谢、二萜生物合成、类黄酮生物合成、脂肪酸延长、光合作用、吲哚生物碱生物合成等途径。

关键词: 江西铅山红芽芋, 江西铅山青秆芋, 转录组分析

Abstract: In order to explore the molecular differences between Jiangxi Yanshan red bud taro and Jiangxi Yanshan green stem taro, the transcriptome analysis was carried out on the plantlets of Jiangxi Yanshan red bud taro (HYY group) and Jiangxi Yanshan green stem taro (QGY group). The results showed that the Clean reads of HYY group was 41 298 676, and the GC content was 53.09%; the Clean reads of QGY group was 45 551 860, and the GC content was 51.17%. In HYY group and QGY group, the pair value of expression FPKM was between 0-2, and the expression density was between 0-0.7. The number of common genes expressed in HYY group and QGY group was 14 038, the number of genes independently expressed in HYY group was 17 843, the number of genes independently expressed in QGY group was 18 634. The correlation coefficient of the expression of HYY group and QGY group was 0.035, and the correlation between samples was poor. There were 32 555 differential expressed genes (DEG) in HYY group and QGY group. Compared with QGY group, there were 15 887 up-regulated genes and 16 668 down regulated genes in HYY group. Go enrichment analysis showed that differential genes were mainly annotated to polysaccharide metabolic process, gene silencing, pectin catabolic process, carbohydrate metabolic process, auxin-activated signaling pathway, extracellular region, intrinsic component of membrane, integral component of membrane, membrane part, apoplast, hydrolase activity, acting on glycosyl bonds, hydrolase activity, hydrolyzing O-glycosyl compounds, protein dimerization activity, DNA binding, oxidoreductase activity, acting on diphenols and related substances as donors and other functions. KEGG enrichment analysis showed that the differential genes were mainly annotated to plant hormone signal transduction, plant-pathogen interaction, cysteine and methionine metabolism, phenylpropanoid biosynthesis, cortisol synthesis and secretion, peroxisome, MAPK signaling pathway-plant, mismatch repair, starch and sucrose metabolism, sulfur metabolism, pentose and glucuronate interconversions, C-type lectin receptor signaling pathway, glycerolipid metabolism, biosynthesis of unsaturated fatty acids, alpha-linolenic acid metabolism, diterpenoid biosynthesis, flavonoid biosynthesis, fatty acid elongation, photosynthesis, indole alkaloid biosynthesis and other pathways. The results of this study will provide reference for variety identification and molecular breeding of Jiangxi red bud taro and Jiangxi green stem taro.

Key words: Jiangxi Yanshan red bud taro, Jiangxi Yanshan green stem taro, transcriptome analysis

中图分类号:

S532

尹明华, 曹晴, 陈红, 邓思宇, 邓燕梅. 江西铅山红芽芋和青秆芋的转录组比较分析[J]. 浙江农业学报, 2020, 32(9): 1533-1543.

YIN Minghua, CAO Qing, CHEN Hong, DENG Siyu, DENG Yanmei. Transcriptome analysis of red bud taro and green stem taro in Yanshan, Jiangxi Province[J]. , 2020, 32(9): 1533-1543.

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江西铅山红芽芋和青秆芋的转录组比较分析

Transcriptome analysis of red bud taro and green stem taro in Yanshan, Jiangxi Province

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