关键词: 沙眼衣原体主要外膜蛋白, 单克隆抗体, 免疫层析试纸条

Abstract: To prepare monoclonal antibodies (McAb) against Chlamydia trachomatis (CT) and establish the Chlamydia trachomatis GICA rapid detection method. Balb/c mice were injected with purified major outer membrane protein (MOMP) of Chlamydia trachomatis. Spleen cells from immunized mice were fused with SP2/0 myeloma cells. Hybirdoma cells which secrete antibodies against MOMP were selected and then identified. Three monoclonal antibodies which had very high affinity to MOMP were obtained. The isotypes of McAbs were IgG1， IgG2b and IgM. The above McAbs had no crossreaction with Chlamydia psittaci， Chlamydia pneumoniae and Mycoplasma pneumoniae. Two McAbs with good pairing were selected to prepare GICA strip. The strip made up of these McAbs can be applied to detect CT. The sensitivity by GICA strip for detecting MOMP was about 50 ng·mL-1.

Key words: Chlamydia trachomatis major outer membrane protein, monoclonal antibody, munochromatographic test strip