响应面结合主成分分析筛选烟草种子最佳引发条件

浙江农业学报

响应面结合主成分分析筛选烟草种子最佳引发条件

1云南省烟草农业科学研究院，云南玉溪 653100；2玉溪中烟种子有限责任公司，云南玉溪 653100；3浙江大学农业与生物技术学院种子科学中心，浙江杭州 310058

出版日期:2015-01-25 发布日期:2015-01-17

Combining response surface analysis and principal component analysis to optimize priming condition in tobacco seeds

1 Yunnan Academy of Tobacco Agricultural Sciences， Yuxi 653100， China; 2 Yuxi Zhongyan Tobacco Seed Limited Liability Company，Yuxi 653100， China；3 Seed Science Center， College of Agriculture and Biotechnology， Zhejiang University， Hangzhou 310058， China

Online:2015-01-25 Published:2015-01-17

摘要/Abstract

摘要： 以红花大金元和云烟97种子为材料，采用响应面试验设计和主成分分析方法，通过20个处理组合对烟草种子进行引发处理，对烟草种子引发时间、引发温度和引发液pH三个因素组合进行优化筛选。然后进行标准发芽试验，以发芽结束后的幼苗全苗长、苗干重、苗鲜重和活力指数为响应量进行响应面和主成分分析。筛选到最佳的红花大金元种子引发条件为引发时间49 h，引发温度30℃，引发pH为5；最佳的云烟97种子引发条件为引发时间45 h，引发温度21℃，引发pH为6。

关键词: 烟草种子, 响应面设计, 引发条件

Abstract: Response surface analysis （RSA） and principal component analysis （PCA） were used to optimize the combination of priming time， priming temperature and the pH values of priming solution in tobacco seeds of cv. Honghua Dajinyuan and Yunyan 97. According to the RSA design， the tobacco seeds were treated by 20 combinations. After that， the germination test of treated seeds was conducted; seedling height， dry weight， fresh weight and vigor index were measured and analyzed by RSA and PCA. Finally， the optimal combination of priming time， priming temperature and the pH values in priming solution were obtained， priming time was 49 h and 45 h， priming temperature was 30℃ and 21℃ and pH values was 5 and 6 for Honghua Dajinyuan and for Yunyan97， respectively.

Key words: tobacco seeds, response surface analysis, priming condition

马文广1，2，利站3，郑昀晔1，2，田艺心1，2，关亚静3，朱丽伟3，胡晋3，*. 响应面结合主成分分析筛选烟草种子最佳引发条件[J]. 浙江农业学报.

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