浙江农业学报

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芦竹种质资源的RAPD分析

  

  1. (1 福建农林大学 动物科学学院, 福建 福州 350000;2 国家菌草工程技术研究中心,福建 福州 350000)
  • 出版日期:2015-04-25 发布日期:2015-04-30

RAPD analysis of genetic relationship of 9 varieties of Arundo donax L.

  1. (1 Fujian Agricultural and Forestry University, Fuzhou 350000, China;2 China National Engineering Research Center of Juncao Technology, Fuzhou 350000, China)
  • Online:2015-04-25 Published:2015-04-30

摘要: 采用RAPD标记分析9 份芦竹属牧草种质的遗传多样性及亲缘关系,为芦竹种质资源的开发利用提供基础信息。结果表明:13条引物在9份材料间共扩增出126条条带,其中113条具有多态性,多态位点百分率为89.7%,各引物扩增条带数为7~14条不等,遗传距离范围为:0~1.039 1,平均值为0.399 6;遗传相似系数为0.309 5~1.000 0,平均为0.734 3。采用非加权类平均法(UPMGA)对统计数据进行聚类分析,遗传距离0.700 0的聚类结果表明:9份材料大致可以分为三类:绿洲5号、绿洲6号各单独聚为一类,与其他材料相似度较低。聚类结果与地理距离没有相关性,没有体现出一定的地域分布规律。

关键词: 芦竹, 遗传多态性, RAPD分析

Abstract: In this study, genetic diversity and relationships of 9 different Arundo donax L. germplasms were analyzed using random amplified polymorphic DNA (RAPD) markers in order to provide essential information for hybridization. RAPD analysis demonstrated that 13 random primers produced 126 loci among which 113 were polymorphic loci, the percentage of polymorphic loci was 897%, and each primer produced 7-14 bands. Genetic distance changed from 0 to 1039 1, with the average value of 0399 6, and the genetic similarity was among the range of 0309 5 to 1000 0, with the average value of 0734 3. According to clustering analysis diagram drawn using UPGMA, the 9 materials were classified into three groups by 07: lyuzhouNo.5 and lyuzhouNo.6 was separated from other materials. The cluster results showed that genetic distance was not related to geographic distance, which cant reflect a certain geographic distribution.

Key words: Arundo donax L.;genetic diversity, RAPD analysis