两种抑菌剂对马来眼子菜开放式组织培养的效应

浙江农业学报

两种抑菌剂对马来眼子菜开放式组织培养的效应

（1上海海洋大学水产与生命学院，上海 201306；2水域环境生态上海高校工程研究中心，上海 201306）

出版日期:2015-04-25 发布日期:2015-04-30

Effect of two bacteriostatic agents on open tissue culture of Potamogeton malaianus

（1 College of Fisheries and Life Science， Shanghai Ocean University， Shanghai 201306， China;2 Engineering Research Center for Water Environment Ecology in Shanghai， Shanghai 201306， China）

Online:2015-04-25 Published:2015-04-30

摘要/Abstract

摘要： 沉水植被的种苗快速规模化繁殖一直是困扰沉水植被恢复，以及水体环境生态修复、改善水质的难点之一，应用低成本、高效的开放式组织培养技术可以实现种苗的快速规模化，抑菌剂的筛选是组织培养的关键技术。试验研究了MS培养基中不同浓度的代森锰锌和凯松，在沉水植物马来眼子菜（Potamogeton malaianus）开放式组织培养中的抗菌作用。结果表明：代森锰锌、凯松均可作为开放式植物组织培养的抑菌剂；代森锰锌浓度在0.070～0.100 g·L-1抑菌效果较理想；凯松浓度在01%抑菌效果较理想，具有良好的应用价值。

关键词: 马来眼子菜, 开放式组织培养, 代森锰锌, 凯松

Abstract: Rapid large\|scale propagation of submerged vegetation seedlings has been one of the difficulties in submerged vegetation restoration， ecological restoration and the aquatic environment. According to the low cost and efficient open tissue culture technology application， bacteriostatic agent selection was the key technology. Mancozeb and Kathon with different concentrations were added to MS medium to study their antibacterial action in open tissue culture of Potamogeton malaianus. The results showed that Mancozeb and Kathon showed ideal bacteriostatic effects on Potamogeton malaianus with the concentrations of 0.07-0.10·L-1 and 0.1%， respectively， which have good application value.

Key words: Potamogeton malaianus, open tissue culture, Mancozeb, Kathon

陈晓君1，张饮江1，2，*，张雨婷1，李博炎1，宋盈颖1，王芳1，翟斯凡1，花文凤1. 两种抑菌剂对马来眼子菜开放式组织培养的效应[J]. 浙江农业学报.