温度对文蛤生理代谢的影响

doi:10.3969/j.issn.1004-1524.2020.09.07

浙江农业学报 ›› 2020, Vol. 32 ›› Issue (9): 1581-1590.DOI: 10.3969/j.issn.1004-1524.2020.09.07

温度对文蛤生理代谢的影响

胡高宇^1,2, 张翔^2,3, 林兴管^2,3, 蔡逸龙^2,3, 蔡景波^2,3, 肖国强^1,2,3, 柴雪良^1,2,3,*

1.上海海洋大学水产科学国家级实验教学示范中心,上海 201306;
2.浙江省海洋水产养殖研究所,浙江温州 325005;
3.浙江省近岸水域生物资源开发与保护重点实验室,浙江温州 325005

收稿日期:2020-04-07 出版日期:2020-09-25 发布日期:2020-10-10
通讯作者: *柴雪良,E-mail:cxl-5888@163.com
作者简介:胡高宇(1994—),男,浙江台州人,硕士研究生,研究方向为养殖生态学。E-mail:hgu150@126.com
基金资助:
浙江省重点研发计划(2018C02039,2019C02045);现代农业产业技术体系建设专项(CARS-49)

Effect of temperature on physiology metabolism of Meretrix petechialis

HU Gaoyu^1,2, ZHANG Xiang^2,3, LIN Xingguan^2,3, CAI Yilong^2,3, CAI Jingbo^2,3, XIAO Guoqiang^1,2,3, CHAI Xueliang^1,2,3,*

1. National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China;
2. Zhejiang Mariculture Research Institute, Wenzhou 325005, China;
3. Zhejiang Key Laboratory of Exploitation and Preservation of Coastal Bioresource, Wenzhou 325005, China

Received:2020-04-07 Online:2020-09-25 Published:2020-10-10

摘要/Abstract

摘要： 以文蛤(Meretrix petechialis)为实验对象,采用实验室静水法和间歇式呼吸测量法,通过测定摄食率、耗氧率、排粪率、排氨率等生理参数,研究了不同水温下不同规格文蛤的生理代谢情况。结果显示:供试文蛤的摄食率、排氨率、排粪率、耗氧率分别在25~30、20~30、15~20、35 ℃存在最大值。随水温升高,文蛤摄食率和排氨率先增加后下降,而排粪率逐渐降低,耗氧率逐渐升高。实验条件下,大、中、小规格的文蛤(壳长范围30~46 mm)摄食率为0.015~0.043 mg·g^-1·h^-1,排氨率为1.26~135.25 μg·g^-1·h^-1,排粪率为2.173~15.908 μg·g^-1·h^-1,耗氧率为0.290~1.780 mg·g^-1·h^-1。研究结果可为探明文蛤在不同季节的生理代谢规律、指导文蛤养殖产业发展提供借鉴。

关键词: 文蛤, 摄食排泄, 呼吸耗氧, 间歇式呼吸测量法

Abstract: In the present study, Meretrix petechialis was selected as the study object. Based on the laboratory still water method and intermittent respiration measurement method, the effects of different temperature on ingestion rate, oxygen consumption rate, faecal egestion rate, and ammonia excretion rate of Meretrix petechialis were explored. It was shown that the maximum values of ingestion rate, ammonia excretion rate, faecal egestion rate and oxygen consumption rate were found under 25-30, 20-30, 15-20, and 35 ℃, respectively. With the elevated water temperature, the ingestion rate and ammonia excretion rate of Meretrix petechialis increased first and then decreased, while the faecal egestion rate gradually decreased, yet oxygen consumption rate increased. Under the experiment conditions, the variation ranges of ingestion rate, ammonia excretion rate, faecal egestion rate and oxygen consumption rate were 0.015-0.043 mg·g^-1·h^-1, 1.26-135.25 μg·g^-1·h^-1, 2.173-15.908 μg·g^-1·h^-1 and 0.290-1.780 mg·g^-1·h^-1, respectively. These results could provide references for the study of physiological metabolism of Meretrix petechialis in different seasons and guide the development of corresponding mariculture.

Key words: Meretrix petechialis, ingestion and excretion, oxygen consumption, intermittent respiration measurement method

中图分类号:

S917.4

胡高宇, 张翔, 林兴管, 蔡逸龙, 蔡景波, 肖国强, 柴雪良. 温度对文蛤生理代谢的影响[J]. 浙江农业学报, 2020, 32(9): 1581-1590.

HU Gaoyu, ZHANG Xiang, LIN Xingguan, CAI Yilong, CAI Jingbo, XIAO Guoqiang, CHAI Xueliang. Effect of temperature on physiology metabolism of Meretrix petechialis[J]. , 2020, 32(9): 1581-1590.

参考文献

[1] 常亚青, 王子臣. 贝类生物能量学研究进展[J]. 海洋科学, 1996, 20(6): 25-30.
CHANG Y Q, WANG Z C.Advancements of molluscan bioenergetics[J]. Marine Sciences, 1996, 20(6): 25-30. (in Chinese)
[2] 徐晴晴, 赵晟. 海水盐度对厚壳贻贝呼吸和钙化的影响[J]. 浙江海洋大学学报(自然科学版), 2018, 37(5): 400-404.
XU Q Q, ZHAO S.Effects of seawater salinity on respiration and calcification of Mytilus edulis[J]. Journal of Zhejiang Ocean University(Natural Science), 2018, 37(5): 400-404.(in Chinese with English abstract)
[3] 饶科, 黄明坚, 章逃平, 等. 盐度与pH对3种南方贝类呼吸率和钙化率的影响[J]. 水生态学杂志, 2014, 35(4): 74-80.
RAO K, HUANG M J, ZHANG T P, et al.Effects of salinity and pH on respiration and calcification of three kinds of shellfish in Southern China[J]. Journal of Hydroecology, 2014, 35(4): 74-80.(in Chinese with English abstract)
[4] 张明亮, 邹健, 方建光, 等. 海洋酸化对栉孔扇贝钙化、呼吸以及能量代谢的影响[J]. 渔业科学进展, 2011, 32(4): 48-54.
ZHANG M L, ZOU J, FANG J G, et al.Impacts of marine acidification on calcification, respiration and energy metabolism of Zhikong scallop Chlamys farreri[J]. Progress in Fishery Sciences, 2011, 32(4): 48-54.(in Chinese with English abstract)
[5] 王俊, 姜祖辉, 唐启升. 栉孔扇贝耗氧率和排氨率的研究[J]. 应用生态学报, 2002, 13(9): 1157-1160.
WANG J, JIANG Z H, TANG Q S.Oxygen consumption and ammonia-N excretion rates of Chlamys farreri[J]. Chinese Journal of Applied Ecology, 2002, 13(9): 1157-1160.(in Chinese with English abstract)
[6] 种金豆, 李琪, 王涛. 长牡蛎壳黑选育品系和野生群体在摄食和代谢方面的比较[J]. 水产学报, 2019, 43(4): 1038-1047.
CHONG J D, LI Q, WANG T.Comparison of feeding and metabolism between black-shell strain and wild population of the Pacific oyster(Crassostrea gigas)[J]. Journal of Fisheries of China, 2019, 43(4): 1038-1047.(in Chinese with English abstract)
[7] 印丽云. 近江牡蛎代谢生理及能量收支的初步研究[D]. 石家庄: 河北师范大学, 2012.
YIN L Y.Studies on metabolic physiology and energy budget of Crassostrea hongkongensis[D]. Shijiazhuang: Hebei Normal University, 2012.(in Chinese with English abstract)
[8] 冀德伟, 陈琛, 李婷婷, 等. 盐度胁迫对泥蚶能量代谢的影响[J]. 水产科学, 2016, 35(5): 504-509.
JI D W, CHEN C, LI T T, et al.Influences of salinity stress on energy metabolism in blood clam Tegillarca granosa[J]. Fisheries Science, 2016, 35(5): 504-509.(in Chinese with English abstract)
[9] 郭晓亮. 中韩两国魁蚶生理代谢和生长特性比较研究[D]. 上海: 上海海洋大学, 2016.
GUO X L.A comparative study on physiological metabolism and growth characteristics of Scapharca broughtonii from China and south Korea[D]. Shanghai: Shanghai Ocean University, 2016.(in Chinese with English abstract)
[10] STEFFENSEN J F.Some errors in respirometry of aquatic breathers: how to avoid and correct for them[J]. Fish Physiology and Biochemistry, 1989, 6(1): 49-59.
[11] THORP J H, COVICH A P.Introduction to freshwater invertebrates[M]//Ecology and classification of North American freshwater invertebrates. Amsterdam: Elsevier, 2001: 1-18.
[12] DU S N N, MCCALLUM E S, VASEGHI-SHANJANI M, et al. Metabolic costs of exposure to wastewater effluent lead to compensatory adjustments in respiratory physiology in bluegill sunfish[J]. Environmental Science & Technology, 2018, 52(2): 801-811.
[13] BLAŽEK R, POLACIK M, KACER P, et al. Repeated intraspecific divergence in life span and aging of African annual fishes along an aridity gradient[J]. Evolution, 2017, 71(2): 386-402.
[14] SUI Y M, KONG H, HUANG X Z, et al.Combined effects of short-term exposure to elevated CO₂ and decreased O₂ on the physiology and energy budget of the thick shell mussel Mytilus coruscus[J]. Chemosphere, 2016, 155: 207-216.
[15] KIM T W, PARK S, SIN E.Differential influences of warming and deoxygenation on the survival, emergence, and respiration of cosmopolitan clams[J]. Ecology and Evolution, 2018, 8(10): 4860-4866.
[16] 栗志民, 刘志刚, 徐法军, 等. 体重、温度和盐度对皱肋文蛤耗氧率和排氨率的影响[J]. 海洋科学进展, 2011, 29(4): 512-520.
LI Z M, LIU Z G, XU F J, et al.Effects of body weight, temperature and salinity on oxygen consumption and ammonia excretion rates of Meretrix lyrata[J]. Advances in Marine Science, 2011, 29(4): 512-520.(in Chinese with English abstract)
[17] 魏伟. 环境胁迫对文蛤摄食和代谢的影响研究[D]. 上海: 上海海洋大学, 2016.
WEI W.Study on feeding and metabolism of Meretrix meretrix under environmental stress[D]. Shanghai: Shanghai Ocean University, 2016.(in Chinese with English abstract)
[18] 杨杰青. 盐度、pH对文蛤能量代谢和基因表达的影响[D]. 上海: 上海海洋大学, 2016.
YANG J Q.Effects of salinity and pH on energy metabolism and gene expression of Meretrix meretrix[D]. Shanghai: Shanghai Ocean University, 2016.(in Chinese with English abstract)
[19] 薛素燕,李加琦,李阳,等.短期酸化对魁蚶摄食、呼吸代谢及能量收支的影响[J/OL].渔业科学进展,[2020-04-07].https://doi.org/10.19663/j.issn2095-9869.20190422001.
XUE S Y, LI J Q, LI Y, et al.Effects of short-term acidification on feeding, respiratory metabolism,and energy budget of Scapharca broughtonii[J/OL]. Progress in Fishery Sciences,[2020-04-07].https://doi.org/10.19663/j.issn2095-9869.20190422001. (in Chinese with English abstract)
[20] JØRGENSEN C B, LARSEN P S, RIISGÅRD H U. Effects of temperature on the mussel pump[J]. Marine Ecology Progress Series, 1990, 64: 89-97.
[21] 彭建华, 陈文祥, 栾建国, 等. 温度、pH对二种淡水贝类滤水率的影响[J]. 动物学杂志, 2004, 39(6): 2-6.
PENG J H, CHEN W X, LUAN J G, et al.The effects of temperature and pH on the filtration rates of two species of freshwater mollusks[J]. Chinese Journal of Zoology, 2004, 39(6): 2-6.(in Chinese with English abstract)
[22] VAN ERKOM SCHURINK C, GRIFFITHS C L. Physiological energetics of four South African mussel species in relation to body size, ration and temperature[J]. Comparative Biochemistry and Physiology Part A: Physiology, 1992, 101(4): 779-789.
[23] WILBUR A E, HILBISH T J.Physiological energetics of the ribbed mussel Geukensia demissa(Dillwyn) in response to increased temperature[J]. Journal of Experimental Marine Biology and Ecology, 1989, 131(2): 161-170.
[24] 董波, 薛钦昭, 李军. 温度对菲律宾蛤仔滤食率、清滤率和吸收率的影响[J]. 海洋水产研究, 2000, 21(1): 37-42.
DONG B, XUE Q Z, LI J.The effect of temperature on the filtration rate, clearance rate and absorption efficiency of Manila clam, Ruditapes philippinarum[J]. Marine Fisheries Research, 2000, 21(1): 37-42.(in Chinese with English abstract)
[25] 栗志民, 刘志刚, 邓海东. 温度和盐度对企鹅珍珠贝清滤率、滤食率、吸收率的影响[J]. 水产学报, 2011, 35(1): 96-103.
LI Z M, LIU Z G, DENG H D.Effects of temperature and salinity on clearance rate, filtration rate and absorption efficiency of Pteria penguin[J]. Journal of Fisheries of China, 2011, 35(1): 96-103. (in Chinese with English abstract)
[26] LAING I, UTTING S D, KILADA R W S. Interactive effect of diet and temperature on the growth of juvenile clams[J]. Journal of Experimental Marine Biology and Ecology, 1987, 113(1): 23-38.
[27] 何苗, 周凯, 么宗利, 等. 饵料浓度、温度对缢蛏能量代谢的影响[J]. 海洋学报, 2017, 39(8): 129-135.
HE M, ZHOU K, YAO Z L, et al.Effects of algae concentration and temperature on energy metabolism of Sinonovacula constricta[J]. Acta Oceanologica Sinica, 2017, 39(8): 129-135. (in Chinese with English abstract)
[28] WIDDOWS J.Physiological indices of stress in Mytilus edulis[J]. Journal of the Marine Biological Association of the United Kingdom, 1978, 58(1): 125-142.
[29] 刘英杰. 青蛤摄食生理和代谢生理以及能量收支的基础研究[D]. 青岛: 中国海洋大学, 2005.
LIU Y J.Studies on the ingestive physiology and metabolic physiology of Cyclina sinensis and its enegy budget[D]. Qingdao: Ocean University of China, 2005.(in Chinese with English abstract)
[30] 李金碧. 环境因子对栉江珧能量收支的影响[D]. 武汉: 华中农业大学, 2009.
LI J B.Effects of environmental factors on the energy budget of pen shell Atrina pectinata[D]. Wuhan: Huazhong Agricultural University, 2009.(in Chinese with English abstract)
[31] 焦海峰, 严巧娜, 郑丹, 等. 温度和盐度对埋栖性双壳类泥蚶(Tegillarca granosa)呼吸、排泄的影响[J]. 海洋与湖沼, 2015, 46(6): 1333-1338.
JIAO H F, YAN Q N, ZHENG D, et al.Effects of temperature and salinity on oxygen consumption and ammonia excretion of muddy shellfish Tegillarca granosa[J]. Oceanologia et Limnologia Sinica, 2015, 46(6): 1333-1338.(in Chinese with English abstract)
[32] BOUGRIER S, GEAIRON P, DESLOUS-PAOLI J M, et al. Allometric relationships and effects of temperature on clearance and oxygen consumption rates of Crassostrea gigas(Thunberg)[J]. Aquaculture, 1995, 134(1/2): 143-154.
[33] 范建勋. 文蛤能量代谢的研究[D]. 宁波: 宁波大学, 2010.
FAN J X.Studies on metabolism of Meretrix meretrix Linnaeus[D]. Ningbo: Ningbo University, 2010.(in Chinese with English abstract)
[34] 王盛青, 尤宏争, 邵蓬, 等. 温度和盐度对文蛤耗氧率、排氨率、滤食率的影响[J]. 经济动物学报, 2016, 20(2): 69-73.
WANG S Q, YOU H Z, SHAO P, et al.Effect of temperature and salinity on oxygen consumption rate, ammonia excretion rates and ingestion rate of Meretrix meretrix[J]. Journal of Economic Animal, 2016, 20(2): 69-73. (in Chinese with English abstract)
[35] 冯建彬, 王美珍, 陈汉春, 等. 温度和规格对文蛤耗氧率的影响[J]. 上海水产大学学报, 2004, 13(2): 126-129.
FENG J B, WANG M Z, CHEN H C, et al.Effects of temperature and body size on oxygen consumption rate of Meretrix meretrix[J]. Journal of Shanghai Fisheries University, 2004, 13(2): 126-129.(in Chinese with English abstract)
[36] BAYNE B L, NEWELL R C.Physiological energetics of marine molluscs[M]//The mollusca. Amsterdam: Elsevier, 1983: 407-515.
[37] 姜祖辉, 王俊. 温度和规格对毛蚶耗氧率和排氨率的影响[J]. 青岛大学学报(自然科学版), 1999, 12(1): 75-79.
JIANG Z H, WANG J.Effects of temperature and body size on oxygen consumption rate and ammonia excretion rate of Arca subcrenata[J]. Journal of Qingdao University(Natural Science), 1999, 12(1): 75-79.(in Chinese with English abstract)
[38] WIDDOWS J, BAYNE B L.Temperature acclimation of Mytilus edulis with reference to its energy budget[J]. Journal of the Marine Biological Association of the United Kingdom, 1971, 51(4): 827-843.
[39] CONOVER R J, CORNER E D S. Respiration and nitrogen excretion by some marine zooplankton in relation to their life cycles[J]. Journal of the Marine Biological Association of the United Kingdom, 1968, 48(1): 49-75.
[40] IKEDA T. Nutritional ecology of marine zooplankton[EB/OL]. (2014-05-29)[2020-04-07]. https://www.researchgate.net/publication/37559088_Nutritional_Ecology_of_Marine_Zooplanktonlink00b7d524211d5ad7e5000000/download.
[41] MAYZAUD P.Respiration and nitrogen excretion of zooplankton IV: the influence of starvation on the metabolism and the biochemical composition of some species[J]. Marine Biology, 1976, 37(1): 47-58.
[42] 杨红生, 张涛, 王萍, 等. 温度对墨西哥湾扇贝耗氧率及排泄率的影响[J]. 海洋学报, 1998, 20(4): 91-96.
YANG H S, ZHANG T, WANG P, et al.Effects of temperature on oxygen consumption and ammonia-N excretion of the Southern Bay-scallop Argopecten irradians concentricus[J]. Acta Oceanologica Sinica, 1998, 20(4): 91-96. (in Chinese with English abstract)

温度对文蛤生理代谢的影响

Effect of temperature on physiology metabolism of Meretrix petechialis

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 7

编辑推荐

Metrics

本文评价

[1]	卢祎, 高有领, 王水涛, 何盛盛. MicroRNA-499对中华鳖脂类代谢相关基因表达的影响[J]. 浙江农业学报, 2020, 32(5): 798-803.
[2]	张爱菊, 郝雅宾, 郭爱环, 刘金殿, 练青平, 周志明. 基于高通量测序技术的鱼类环境DNA研究中通用引物的筛选验证[J]. 浙江农业学报, 2019, 31(10): 1615-1623.
[3]	潘传燕, 林勇, 冯鹏霏, 张永德, 罗洪林. 尼罗罗非鱼Hsc70的原核表达和多克隆抗体制备[J]. 浙江农业学报, 2019, 31(8): 1272-1279.
[4]	易乐飞, 穆亮亮, 仲改. 凡纳滨对虾总RNA完整性的正确评估[J]. 浙江农业学报, 2016, 28(12): 2007-2013.
[5]	刘士力, 张爱菊, 练青平, 吴卫君, 贾永义, 蒋文枰, 李飞. 泥鳅和大鳞副泥鳅细胞色素P450c17-Ⅰ(CYP17-Ⅰ)基因的克隆及组织表达分析[J]. 浙江农业学报, 2016, 28(11): 1853-1861.
[6]	李兆双, 王喜男, 王鹏, 陈尚钘, 范国荣, 王宗德. 天然柠檬醛衍生物对食品腐败细菌的抑制活性[J]. 浙江农业学报, 2016, 28(11): 1928-1933.
[7]	岳蒙蒙, 赵金良, 唐首杰, 王燕, 赵永华. 性成熟前期尼罗罗非鱼雌雄性别生长差异与性类固醇激素水平比较[J]. 浙江农业学报, 2016, 28(10): 1678-1686.