›› 2011, Vol. 23 ›› Issue (2): 0-343.

• 植物保护 •    

生化因子对丛枝菌根真菌Glomus intraradices产孢和侵染的影响

马继芳,金海如*,张德兴,戚丽娜
  

  1. 浙江师范大学 化学与生命科学学院,浙江 金华 321004
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2011-03-25 发布日期:2011-03-25

Effect of biochemical factors on the spore yields and infection rates of Glomus intraradices

MA Ji-fang;JIN Hai-ru*;ZHAND De-xing;QI Li-na   

  1. College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2011-03-25 Published:2011-03-25

摘要: 以高粱为宿主,采用盆栽试验,研究生化因子对丛枝菌根真菌Glomus intraradices产孢和侵染的影响。结果表明:单一有机氮源和活性物质能显著提高G. intraradices产孢和侵染,酒石酸铵(0.5%)、甘氨酸(1%)和根浸出液(2 mL),可分别使每160 g沙样孢子产量提高至382,302和328个,对照仅为155个;甘氨酸(0.5%,1.0%)、酒石酸铵(0.5%)、腐殖酸铵(0.5%,1.0%,2.0%)和根浸出液(2,3,5 mL)处理,可使根侵染率分别提高至53.7%,52.8%,43.9%,59.4%,48.9%,57.7%,64.9%,55.9%,52.9%(对照仅为27.3%);单一碳源(葡萄糖、蔗糖)则对G. intraradices产孢和侵染率都没有显著影响;不同的氮源+碳源(NH4Cl+葡萄糖)组合对G. intraradices产孢和侵染率有不同影响,当C、N比值为4∶1,1∶2时,显著提高G. intraradices产孢,可分别使每20 g沙样产孢量可分别达到2227,2458个(对照仅为157个)。当C、N比值为1∶2时,侵染率为2.4%,显著低于对照19.3%;比值为4∶1时,对侵染率没有显著影响。生化因子对G.intraradices产孢和侵染的作用特点不同。

关键词: 丛枝菌根真菌, 孢子量, 侵染率, 生化因子

Abstract: Effect of biochemical factors on G. intraradices infection rates and spore yields were investigated in pot culture using sorghum (Sorghum vulgare Pers.) as the host plants. The results showed that G. intraradices infection rates and spore yields were significantly improved by biochemical factors in a debita spissitudine of single organic nitrogen and active substance. After the treatments of ammonium tartrate(0.5%), glycine (1%) and root lixivium (2 mL), spore yields of every 160 g sand sample were increased to 382 spores, 302 spores and 328 spores (the control was 155 spores), respectively. After the treatments of glycine (0.5%, 1%), ammonium tartrate (0.5%), ammonium humate (0.5%, 1%, 2%) and root lixivium (2, 3, 5 mL) , G. intraradices infection rates reached 537%, 52.8%, 43.9%, 59.4%, 48.9%, 57.7%, 64.9%, 55.9%, 52.9% (the control was 27.3%), respectively. Single C source (glucose, sucrose) had no influence on G. intraradices spore yields and infection rates. N source plus C source (NH4Cl plus glucose) in the C/N ratio of 4∶1 or 1∶2, spore yields of every 20 g sand sample gave rise to 2 227 spores and 2 458 spores (the control was 157 spores), respectively. While C/N ratio was 1∶2, the infection rates was 2.4% (the control was 19.3%). The ratio of 4∶1 had no remarkable effect. The function characteristics of biochemical factors on the infection rates and spore yields of G.intraradices were different.

Key words: Glomus intraradices, spore yields, infection rates, biochemistry factor