丛枝菌根真菌作为指示性物种评估转基因作物对土壤微生物影响的研究概述

doi:10.3969/j.issn.1004-1524.2018.07.22

浙江农业学报 ›› 2018, Vol. 30 ›› Issue (7): 1267-1272.DOI: 10.3969/j.issn.1004-1524.2018.07.22

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丛枝菌根真菌作为指示性物种评估转基因作物对土壤微生物影响的研究概述

梁晋刚, 焦悦, 刘鹏程, 张秀杰^*

农业农村部科技发展中心,北京 100176

收稿日期:2017-12-30 出版日期:2018-07-20 发布日期:2018-08-02
通讯作者: 张秀杰,E-mail: zhxj7410@sina.com
作者简介:梁晋刚(1987—),男,山西阳泉人,博士,农艺师,主要从事转基因生物安全评价与检测研究。E-mail: 382408162@qq.com
基金资助:
转基因生物新品种培育科技重大专项(2016ZX08012003)

Arbuscular mycorrhizal fungi as a potential indicator to assess effects of genetically modified crops on soil microorganisms

LIANG Jingang, JIAO Yue, LIU Pengcheng, ZHANG Xiujie^*

Development Center of Science and Technology, Ministry of Agriculture and Rural Affairs, Beijing 100176, China

Received:2017-12-30 Online:2018-07-20 Published:2018-08-02

摘要/Abstract

摘要： 转基因作物与土壤生态系统环境要素紧密相关。土壤微生物作为土壤生态系统的一个重要组成部分,转基因作物大面积种植是否会给土壤微生物带来影响已成为其生态风险评价中不可忽视的一个方面。土壤中的微生物种类繁多,因此在研究时有必要选取一些具有代表性的微生物进行分析。丛枝菌根真菌作为一类重要的土壤环境指示微生物,在改善土壤质量与健康状况、提高生物多样性、促进植物生长等方面发挥着不可替代的作用。文章就主要转基因作物对丛枝菌根真菌的影响和作用机制进行综述,认为可将丛枝菌根真菌作为评价转基因作物对土壤生态系统影响的指示生物。

关键词: 转基因作物, 土壤微生物, 丛枝菌根真菌

Abstract: Genetically modified (GM) crops are closely related to the environmental elements of soil ecosystem, microorganisms are the important components of the soil ecosystem, thus, whether the large-scale plantation of GM crops would affect soil microorganisms has become an important aspect of ecological risk evaluation. There are many kinds of microorganisms in the soil, so it is necessary to select some representative microorganisms as indicators. As an important soil environment indicator, arbuscular mycorrhizal fungi (AMF) play an irreplaceable role in improving soil quality and healthy status, enhancing biological diversity and promoting plant growth. This paper reviewed the influence of main GM crops on AMF, and suggested that AMF could be used as a potential indicator to assess the effects of GM crops on soil microorganisms.

Key words: genetically modified crops, soil microorganisms, arbuscular mycorrhizal fungi

中图分类号:

S154.3

梁晋刚, 焦悦, 刘鹏程, 张秀杰. 丛枝菌根真菌作为指示性物种评估转基因作物对土壤微生物影响的研究概述[J]. 浙江农业学报, 2018, 30(7): 1267-1272.

LIANG Jingang, JIAO Yue, LIU Pengcheng, ZHANG Xiujie. Arbuscular mycorrhizal fungi as a potential indicator to assess effects of genetically modified crops on soil microorganisms[J]. , 2018, 30(7): 1267-1272.

参考文献

[1] 国际农业生物技术应用服务组织. 2016年全球生物技术/转基因作物商业化发展态势[J]. 中国生物工程杂志, 2017, 37(4):1-8.
International Service for the Acquisition of Agri-Biotech Applications. Current development of genetically modified crops globally in 2016[J]. China Biotechnology , 2017, 37(4): 1-8. (in Chinese)
[2] ROMEIS J, BARTSCH D, BIGLER F, et al. Assessment of risk of insect-resistant transgenic crops to nontarget arthropods[J]. Nature Biotechnology , 2008, 26(2): 203-208.
[3] KOLSETH A K, D'HERTEFELDT T, EMMERICH M, et al. Influence of genetically modified organisms on agro-ecosystem processes[J]. Agriculture , Ecosystems & Environment , 2015, 214: 96-106.
[4] NICOLIA A, MANZO A, VERONESI F, et al. An overview of the last 10 years of genetically engineered crop safety research[J]. Critical Reviews in Biotechnology , 2014, 34(1): 77-88.
[5] LIU W. Do genetically modified plants impact arbuscular mycorrhizal fungi?[J]. Ecotoxicology , 2010, 19(2): 229-238.
[6] 梁晋刚, 张正光. 转基因作物种植对土壤生态系统影响的研究进展[J]. 作物杂志, 2017(4):1-6.
LIANG J G, ZHANG Z G. Advance on effects of genetically modified crops on soil ecosystems[J]. Crops , 2017 (4): 1-6. (in Chinese with English abstract)
[7] TURRINI A, SBRANA C, GIOVANNETTI M. Belowground environmental effects of transgenic crops: a soil microbial perspective[J]. Research in Microbiology , 2015, 166(3): 121-131.
[8] JIANG Y, WANG W, XIE Q, et al. Plants transfer lipids to sustain colonization by mutualistic mycorrhizal and parasitic fungi[J]. Science , 2017: 356(6343): 1172-1175.
[9] HANNULA S E, DE BOER W, VAN VEEN J A. Do genetic modifications in crops affect soil fungi? A review[J]. Biology and Fertility of Soils , 2014, 50(3): 433-446.
[10] 任禛, 王建武, 冯远娇,等. 丛枝菌根真菌侵染Bt玉米及对其生长的影响[J]. 生态学杂志, 2011, 30(6):1163-1168.
REN Z, WANG J W, FENG Y J, et al. Effects of arbuscular mycorrhizal fungi colonization on Bt corn growth[J]. Chinese Journal of Ecology , 2011, 30(6):1163-1168. (in Chinese with English abstract)
[11] WENKE L, LIANFENG D. Interactions between Bt transgenic crops and arbuscular mycorrhizal fungi: a new urgent issue of soil ecology in agroecosystems[J]. Acta Agriculturae Scandinavica Section B-Soil and Plant Science , 2008, 58(2): 187-192.
[12] STEFANI F O P, HAMELIN R C. Current state of genetically modified plant impact on target and non-target fungi[J]. Environmental Reviews , 2010, 18: 441-475.
[13] MOHANDASS D, MUTHUKUMAR T. An insight into genetically modified crop-mycorrhizal symbiosis[M]// SINGH D, SINGH H, PRABHA R. Plant-microbe interactions in agro-ecological perspectives. Singapore: Springer, 2017: 403-429.
[14] TURRINI A, SBRANA C, NUTI M P, et al. Development of a model system to assess the impact of genetically modified corn and aubergine plants on arbuscular mycorrhizal fungi[J]. Plant and Soil , 2005, 266(1/2): 69-75.
[15] VIERHEILIG H, ALT M, LANGE J, et al. Colonization of transgenic tobacco constitutively expressing pathogenesis-related proteins by the vesicular-arbuscular mycorrhizal fungus Glomus mosseae [J]. Applied and Environmental Microbiology , 1995, 61(8): 3031-3034.
[16] SCHÜßLER A, SCHWARZOTT D, WALKER C. A new fungal phylum, the Glomeromycota : phylogeny and evolution[J]. Mycological Research , 2001, 105(12): 1413-1421.
[17] REDECKER D, SCHÜßLER A, STOCKINGER H, et al. An evidence-based consensus for the classification of arbuscular mycorrhizal fungi ( Glomeromycota )[J]. Mycorrhiza , 2013, 23(7): 515-531.
[18] LIANG J, MENG F, SUN S, et al. Community structure of arbuscular mycorrhizal fungi in rhizospheric soil of a transgenic high-methionine soybean and a near isogenic variety[J]. PloS One , 2015, 10(12): e0145001.
[19] POWELL J R, GULDEN R H, HART M M, et al. Mycorrhizal and rhizobial colonization of genetically modified and conventional soybeans[J]. Applied and Environmental Microbiology , 2007, 73(13): 4365-4367.
[20] VILVERT R M, AGUIAR D, GIMENES R M T, et al. Residual effect of transgenic soybean in soil microbiota[J]. African Journal of Agricultural Research , 2014, 9(30): 2369-2376.
[21] COLOMBO R P, IBARRA J G, BIDONDO L F, et al. Arbuscular mycorrhizal fungal association in genetically modified drought-tolerant corn[J]. Journal of Environmental Quality , 2017, 46(1): 227-231.
[22] CHEEKE T E, SCHÜTTE U M, HEMMERICH C M, et al. Spatial soil heterogeneity has a greater effect on symbiotic arbuscular mycorrhizal fungal communities and plant growth than genetic modification with Bacillus thuringiensis toxin genes[J]. Molecular Ecology , 2015, 24(10): 2580-2593.
[23] CHEEKE T E, DARBY H, ROSENSTIEL T N, et al. Effect of Bacillus thuringiensis ( Bt ) maize cultivation history on arbuscular mycorrhizal fungal colonization, spore abundance and diversity, and plant growth[J]. Agriculture , Ecosystems & Environment , 2014, 195: 29-35.
[24] CHEEKE T E, CRUZAN M B, ROSENSTIEL T N. Field evaluation of arbuscular mycorrhizal fungal colonization in Bacillus thuringiensis toxin-expressing ( Bt ) and non- Bt maize[J]. Applied and Environmental Microbiology , 2013, 79(13): 4078-4086.
[25] CHEEKE T E, ROSENSTIEL T N, CRUZAN M B. Evidence of reduced arbuscular mycorrhizal fungal colonization in multiple lines of Bt maize[J]. American Journal of Botany , 2012, 99(4): 700-707.
[26] CHEEKE T E, PACE B A, ROSENSTIEL T N, et al. The influence of fertilizer level and spore density on arbuscular mycorrhizal colonization of transgenic Bt 11 maize ( Zea mays ) in experimental microcosms[J]. FEMS Microbiology Ecology , 2010, 75(2): 304-312.
[27] CASTALDINI M, TURRINI A, SBRANA C, et al. Impact of Bt corn on rhizospheric and soil eubacterial communities and on beneficial mycorrhizal symbiosis in experimental microcosms[J]. Applied and Environmental Microbiology , 2005, 71(11): 6719-6729.
[28] 钟旺, 曾慧兰, 王建武. Bt 基因导入对侵染丛枝菌根真菌的玉米生长生理及磷转运基因表达的影响[J]. 中国生态农业学报, 2017, 25(8):1198-1205.
ZHONG W, ZENG H L, WANG J W. Effect of Bt gene insertion on growth, physiology and gene expression of phosphorus transporter gene of corn after arbuscular mycorrhizal fungi colonization[J]. Chinese Journal of Eco-Agriculture , 2017, 25(8): 1198-1205. (in Chinese with English abstract)
[29] VERBRUGGEN E, KURAMAE E E, HILLEKENS R, et al. Testing potential effects of maize expressing the Bacillus thuringiensis Cry1Ab endotoxin (Bt maize) on mycorrhizal fungal communities via DNA-and RNA-based pyrosequencing and molecular fingerprinting[J]. Applied and Environmental Microbiology , 2012, 78(20): 7384-7392.
[30] ZENG H, TAN F, SHU Y, et al. The Cry1Ab protein has minor effects on the arbuscular mycorrhizal fungal communities after five seasons of continuous Bt maize cultivation[J]. PloS One , 2015, 10(12): e0146041.
[31] KNOX O G G, NEHL D B, MOR T, et al. Genetically modified cotton has no effect on arbuscular mycorrhizal colonisation of roots[J]. Field Crops Research , 2008, 109(1/2/3): 57-60.
[32] 刘红梅, 黄永春, 屠臣阳,等. 转双价棉丛枝菌根真菌侵染率与植株养分含量变化的研究[J]. 作物杂志, 2012(1):79-83.
LIU H M, HUANG Y C, TU C Y, et al. Changes of arbuscular mycorrhizal fungi colonization rate and plant nutrient content of transgenic Bt + CpTI cotton[J]. Crops , 2012 (1): 79-83. (in Chinese with English abstract)
[33] 尹念辅, 杨兵, 李铁松, 等. Bt 棉与丛枝菌根真菌对植物根际土壤营养物质和土壤酶活性的影响[C]//第五届全国农业环境科学学术研讨会论文集, 2013: 351-358.
[34] MEYER J B, SONGWILSON Y, FOETZKI A, et al. Does wheat genetically modified for disease resistance affect root-colonizing pseudomonads and arbuscular mycorrhizal fungi?[J]. PloS One , 2013, 8(1):e53825.
[35] KAHLON J G, JACOBSEN H J, CAHILL J F, et al. Antifungal genes expressed in transgenic pea ( Pisum sativum L.) do not affect root colonization of arbuscular mycorrhizae fungi[J]. Mycorrhiza , 2017, 27(7): 683-694.
[36] VIERHEILIG H, ALT M, LANGE J, et al. Colonization of transgenic tobacco constitutively expressing pathogenesis-related proteins by the vesicular-arbuscular mycorrhizal fungus Glomus mosseae [J]. Applied and Environmental Microbiology , 1995, 61(8): 3031-3034.
[37] VIERHEILIG H, ALT M, NEUHAUS J M, et al. Colonization of transgenic Nicotiana sylvestris plants, expressing different forms of Nicotiana tabacum chitinase, by the root pathogen Rhizoctonia solani and by the mycorrhizal symbiont Glomus mosseae [J]. Molecular Plant-Microbe Interactions , 1993, 6(2): 261-264.
[38] KAUR J, FELLERS J, ADHOLEYA A, et al. Expression of apoplast-targeted plant defensin MtDef 4. 2 confers resistance to leaf rust pathogen Puccinia triticina but does not affect mycorrhizal symbiosis in transgenic wheat[J]. Transgenic Research , 2017, 26(1): 37-49.
[39] 刘平, 林会, 赵斌. 转Lj- POLLUX 和Lj- NFR 1 -NFR 5基因的水稻对AM真菌侵染的影响[J]. 土壤学报, 2013, 50(1):208-213.
LIU P, LIN H, ZHAO B. Colonization of AM fungi on transgenic Lj- POLLUX and Lj- NFR 1 -NFR 5 rice[J]. Acta Pedologica Sinica , 2013, 50(1): 208-213. (in Chinese with English abstract)
[40] SHRAWAT A K, CARROLL R T, DEPAUW M, et al. Genetic engineering of improved nitrogen use efficiency in rice by the tissue‐specific expression of alanine aminotransferase[J]. Plant Biotechnology Journal , 2008, 6(7): 722-732.
[41] BIRCH A N E, GRIFFITHS B S, CAUL S, et al. The role of laboratory, glasshouse and field scale experiments in understanding the interactions between genetically modified crops and soil ecosystems: a review of the ECOGEN project[J]. Pedobiologia , 2007, 51(3): 251-260.
[42] HORN P, SCHLICHTING A, BAUM C, et al. Fast and sensitive in vivo studies under controlled environmental conditions to substitute long-term field trials with genetically modified plants[J]. Journal of Biotechnology , 2017, 243: 48-60.
[43] 刘文科, 杜连凤. 转 Bt 基因作物对丛枝菌根真菌的影响研究进展[J]. 中国土壤与肥料, 2007(6):10-13.
LIU W K, DU L F. Advances in effects of Bt transgenic crops on arbuscular mycorrhizal fungi[J]. Soils and Fertilizers Sciences in China , 2007 (6): 10-13.(in Chinese with English abstract)
[44] 焦悦, 梁晋刚, 翟勇. 转基因作物安全评价研究进展[J]. 作物杂志, 2016(5):1-7.
JIAO Y, LIANG J G, ZHAI Y. Progress in safety assessment of genetically modified crops[J]. Crops , 2016 (5): 1-7. (in Chinese with English abstract)
[45] DONEGAN K K, PALM C J, FIELAND V J, et al. Changes in levels, species and DNA fingerprints of soil microorganisms associated with cotton expressing the Bacillus thuringiensis var. kurstaki endotoxin[J]. Applied Soil Ecology , 1995, 2(2): 111-124.
[46] DONEGAN K K, SCHALLER D L, STONE J K, et al. Microbial populations, fungal species diversity and plant pathogen levels in field plots of potato plants expressing the Bacillus thuringiensis var. tenebrionis endotoxin[J]. Transgenic Research , 1996, 5(1): 25-35.
[47] DONEGAN K K, SEIDLER R J, DOYLE J D, et al. A field study with genetically engineered alfalfa inoculated with recombinant Sinorhizobium meliloti : effects on the soil ecosystem[J]. Journal of Applied Ecology , 1999, 36(6): 920-936.
[48] NEWHOUSE A E, SCHRODT F, LIANG H, et al. Transgenic American elm shows reduced Dutch elm disease symptoms and normal mycorrhizal colonization[J]. Plant Cell Reports , 2007, 26(7): 977-987.
[49] KALDORF M, FLADUNG M, MUHS H J, et al. Mycorrhizal colonization of transgenic aspen in a field trial[J]. Planta , 2002, 214(4): 653-660.
[50] SEPPÄNEN S K, PASONEN H L, VAURAMO S, et al. Decomposition of the leaf litter and mycorrhiza forming ability of silver birch with a genetically modified lignin biosynthesis pathway[J]. Applied Soil Ecology , 2007, 36(2/3): 100-106.
[51] TAN F, WANG J, FENG Y, et al. Bt corn plants and their straw have no apparent impact on soil microbial communities[J]. Plant and Soil , 2010, 329(1/2): 349-364.
[52] TAN F, WANG J, CHEN Z, et al. Assessment of the arbuscular mycorrhizal fungal community in roots and rhizosphere soils of Bt corn and their non- Bt isolines[J]. Soil Biology and Biochemistry , 2011, 43(12): 2473-2479.
[53] KURAMAE E E, VERBRUGGEN E, HILLEKENS R, et al. Tracking fungal community responses to maize plants by DNA-and RNA-based pyrosequencing[J]. PloS One , 2013, 8(7): e69973.
[54] ZENG H, TAN F, ZHANG Y, et al. Effects of cultivation and return of Bacillus thuringiensis ( Bt ) maize on the diversity of the arbuscular mycorrhizal community in soils and roots of subsequently cultivated conventional maize[J]. Soil Biology and Biochemistry , 2014, 75: 254-263.
[55] XUE K, SEROHIJOS R C, DEVARE M, et al. Decomposition rates and residue-colonizing microbial communities of Bacillus thuringiensis insecticidal protein Cry3Bb-expressing (Bt) and non-Bt corn hybrids in the field[J]. Applied and Environmental Microbiology , 2011, 77(3): 839-846.
[56] WRÓBEL-KWIATKOWSKA M, TURNAU K, GÓRALSKA K, et al. Effects of genetic modifications to flax ( Linum usitatissimum ) on arbuscular mycorrhiza and plant performance[J]. Mycorrhiza , 2012, 22(7): 493-499.
[57] SERES A, KISS I, NAGY P, et al. Arbuscular mycorrhizal fungi colonisation of Cry 3 toxin-producing Bt maize and near isogenic maize[J]. Plant Soil & Environment , 2014, 60(12): 569-573.
[58] LEE S H, KIM C G, KANG H. Temporal dynamics of bacterial and fungal communities in a genetically modified (GM) rice ecosystem[J]. Microbial Ecology , 2011, 61(3): 646-659.

丛枝菌根真菌作为指示性物种评估转基因作物对土壤微生物影响的研究概述

Arbuscular mycorrhizal fungi as a potential indicator to assess effects of genetically modified crops on soil microorganisms

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