关键词: 绿竹, 种源, ISSR分析, 遗传距离

Abstract: The genetic diversity among different provenances was analyzed by ISSR molecular marker in order to prove relationship among 7 provenances of Dendrocalamopsis oldhami. The results showed that 20 effective primers were screened out from 30 ISSR primers, which amplified a total of 273 sites, among of which 176 sites showed polymorphism, accounted for 64.5% of the total. Cluster analysis showed 7 provenances of Dendrocalamopsis oldhami were divided into 3 groups, genetic distance of different provenances was 0.015-0.465. Genetic relationship of cultivated species of Dendrocalamopsis oldhami was closer, and the countries of origin of Dendrocalamopsis oldhami(Munro)Keng f. from. striata Y.Y.Wang et W.Y.Zhang and Dendrocalamopsis oldhami(Munro)Keng f. f. revolute (W.T.Lin et J.Y.Lin)W.T.Lin were Ruian and Cangnan．

Key words: Dendrocalamopsis oldhami, provenance, ISSR analysis, genetic distance