关键词: 春兰, ABC模型, GLO基因, 实时荧光定量表达

Abstract: In this study, a novel GLO gene was isolated from Cymbidium goeringii by RT-PCR and RACE-PCR techniques. Sequence analysis showed the gene contained an open reading frame of 630 bp encoding a putative protein of 210 amino acids. Homology analysis showed that the deduced protein showed high identity with other PI/GLO proteins from different species, named CgGLO (GenBank accession number HM106984). Phylogenetic tree analysis also indicated that the B-functional gene CgGLO belongs to PI/GLO clade of MADS-box gene family. Real-time quantitative PCR demonstrated that CgGLO was strongly expressed in 2 whorls organ, i.e. lips and petals, much lower in ovary, leaves and sepals, and lowest in root and columns. It supported the modified ABC model by van Tunen. These results also displayed that CgGLO might regulate the floral organ identity and ovary development of C. goeringii．

Key words: Cymbidium goeringii, ABC model, GLO gene, Real-time quantitative PCR