›› 2011, Vol. 23 ›› Issue (4): 0-716.

• 动物科学 •    

青蟹野生与养殖群体遗传多样性的微卫星分析

周宇芳1,舒妙安1,*,赵晓枫2,朱晓宇1   

  1. 1浙江大学 动物科学学院,浙江 杭州 310029;2杭州师范大学 生命与环境科学学院,浙江 杭州 310036
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2011-07-25 发布日期:2011-07-25

Microsatellite analysis on genetic diversity of wild and cultured populations of Scylla paramamosain

ZHOU Yu-fang;SHU Miao-an;*;ZHAO Xiao-feng;ZHU Xiao-yu   

  1. 1College of Animal Science, Zhejiang University, Hangzhou 310029, China;2Institute of Development and Regeneration Biology, Hangzhou Normal University, Hangzhou 310036, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2011-07-25 Published:2011-07-25

摘要: 利用微卫星分子标记对青蟹东海三门湾野生群体和浙江三门养殖群体的遗传多样性进行分析。6个微卫星位点在2个青蟹群体中共检测到66个等位基因,多态信息含量为0.746 ~ 0.895,均表现为高度多态性,可有效应用于青蟹遗传多样性的研究。青蟹野生群体与养殖群体的等位基因数分别为4 ~ 18,6 ~ 14,平均杂合度分别为0.577,0.561,平均多态信息含量分别为0.779,0.828;群体间遗传分化指数FST值为0.0317,遗传相似性指数为0.7930,遗传距离为0.2319。结果表明,青蟹野生与养殖群体的遗传多样性均较丰富,群体间存在一定的遗传分化。

关键词: 青蟹, 野生群体, 养殖群体, 微卫星, 遗传多样性

Abstract: To study the genetic diversity and population structure of the wild Scylla paramamosain population in the Sanmen Bay and the cultured population from Sanmen County, six microsatellite markers were used. A total of 66 alleles were obtained and the polymorphic information content (PIC) value per loci was greater than 0.5, indicating that the six loci were highly polymorphic and can be effectively used to analysis of genetic structure of the crab populations. The numbers of alleles of the wild and cultured populations in six loci ranged from 4 to 18, 6 to 14 respectively. The mean heterozygosity of the two populations were 0.577, 0.561, while the mean PIC value were 0.779, 0.828. The coefficient of genetic differentiation (FST) was 0.0317. The genetic similarity index and genetic distance were 0.7930, 0.2319, respectively. All of these showed that the genetic diversity of the two populations was relatively abundance, and faint genetic differentiation existed between the wild population and cultured population.

Key words: Scylla paramamosain, wild population, cultured population, microsatellite, genetic diversity