›› 2011, Vol. 23 ›› Issue (5): 0-960.

• 植物保护 •    

部分糖类及化感物质对白术白绢病菌与根腐病菌的影响

方丽1,王连平1,任海英1,茹水江1,冯忠民2,王汉荣1,*   

  1. 1浙江省农业科学院 植物保护与微生物研究所,浙江 杭州 310021;2浙江省新昌县农业局,浙江 新昌 312500
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2011-09-25 发布日期:2011-09-25

Effects of some sugars and allelochemicals on biological characteristics of Sclerotium rolfsii Sacc and Fusarium oxysporium in Atractylodes macrocephaia Koidz

FANG Li;WANG Lian-ping;REN Hai-ying;RU Shui-jiang;FENG Zhong-min;WANG Han-rong;*   

  1. 1Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021,China; 2Station of Plant Protection of Xinchang County in Zhejiang, Xinchang 314000,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2011-09-25 Published:2011-09-25

摘要: 研究了部分糖类、氨基酸、酚类、有机酸类、白术汁和白术粉对白术白绢病菌及根腐病菌生长与繁殖的影响。结果表明,蔗糖、葡萄糖、麦芽糖、鼠李糖和甘露糖对白绢病菌和根腐病菌菌丝的生长都具有明显的促进作用,而乳糖、D-果糖、木糖对菌丝的生长有抑制作用。L-组氨酸、L-异亮氨酸、酪氨酸、精氨酸、丝氨酸、脯氨酸和硝酸钾能明显促进白绢病菌和根腐病菌的菌落生长,而天门冬氨酸和半胱氨酸对2种菌均有较强的抑制作用。咖啡酸乙酯和香兰素对白绢病菌和根腐病菌有很强的抑制作用,儿茶素和咖啡酸对白绢病菌却有促进生长的作用,但是多数化感物质对2种菌的生长影响较小。几种浓度的白术汁和白术粉培养基对白绢病菌和根腐病菌的生长有促进作用,其效果与10%和5%的马铃薯培养基相近。不同的碳源、氮源和化感物质对白术白绢病菌和根腐病菌生长的影响是不同的。

关键词: 白术, 糖类, 化感物质, 白绢病菌, 根腐病菌

Abstract: In this study, the effects of sugars, amino acids, phenolic acids, organic acids and the extract and powder of Atractylodes macrocephaia Koidz on the growth and replication of S.rolfsii and F. oxysporium were studied. The results showed that the growth of hypha was promoted by sucrose, glucose, maltose, rhamnose and mannose, but was inhibited by lactose, D (-)-fructose and xylose. The growth of hypha was also promoted by L-histidine, L-isoleucine, tyrosin, arginine, serine, proline and potassium nitrate, but was inhibited strongly by aspartic acid, cysteine. The growth of S. rolfsii was inhibited by caffeic acid, ethyl ester and vanillin but was promoted by catechin and affeic acid. The study also found that most allelochemicals had little influences on the growth of the two pathogens. Finally the growth of pathogen grown on the medium with different concentrations of extract and powder of A. macrocephaia was similar with those on 5% or 10% potato dextrose Agar. In conclusion, different carbon and nitrogen source and allelochemicals had different effects on the growth of the two pathogens.

Key words: Atractylodes macrocephaia Koidz, sugars, allelochemicals, Sclerotium rolfsii Sacc, Fusarium oxysporium