›› 2011, Vol. 23 ›› Issue (5): 0-1022.

• 环境科学 •    

高自由基清除活性竹黄菌株液态发酵工艺的优化

夏更寿

  

  1. 丽水学院,浙江 丽水 323000
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2011-09-25 发布日期:2011-09-25

Optimization of fermentation technology for an anamorphic strain from Shiraia bambusicola with high free radical scavenging activity

XIA Geng-shou   

  1. Lishui University, Lishui 323000,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2011-09-25 Published:2011-09-25

摘要:

以竹黄无性型菌株ZHLS-03为材料,采用单因子和六因素三水平正交试验法,以高生物量为前提,筛选优化高自由基清除活性的液体发酵培养基和发酵条件。结果表明:较理想的培养基组成为K2HPO4 0.3%,KCl 0.15%,MgSO4·7H2O 0.1%,FeSO4·7H2O 0.05%,酵母浸出粉1.5 %,葡萄糖3%;最佳发酵条件是pH值7.0,培养温度30℃。

关键词: 竹黄, 菌丝体, 自由基清除率

Abstract:

Using single factor experiment and orthogonal experiment with six factors and three levels, the submerged culture medium and fermentation conditions for optimization of fermentation of strain ZHLS-03 from Shiraia bambusicola were selected based on high free radical scavenging activity and biomass. The results suggested that the optimum medium components and culture conditions were as follows: K2HPO4 0.3%, KCl 0.15%, MgSO4·7H2O 0.1%, FeSO4·7H2O 0.05%, yeast extract 1.5%, glucose 3%, pH 7.0, culture temperature 30℃. This study will provide a basis for further development and utilization of strain ZHLS-03.

Key words: Shiraia bambusicola P.Henn., mycelium, free radical scavenging rate