鸭坦布苏病毒一步法RTPCR检测方法的建立和应用

›› 2012, Vol. 24 ›› Issue (1): 0-40.

• 动物科学 •

鸭坦布苏病毒一步法RTPCR检测方法的建立和应用

张帅1,2,云涛2,叶伟成2,邓晓辉1,2,崔言顺1,*,华炯钢2,张存2,*,张燕2

1山东农业大学动物医学院,山东泰安 271018；2浙江省农业科学院畜牧兽医研究所,浙江杭州 310021

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2012-01-24 发布日期:2012-01-24

Development and application of one step RTPCR technique for detection of Duck Tembusu virus Abstract: Key words:

ZHANG Shuai;YUN Tao;YE Weicheng;DENG Xiaohui;CUI Yanshun;*;HUA Jionggang;ZHANG Cun;*;ZHANG Yan

1College of Veterinary Medicine, Shandong Agricultural University, Taian 271018,China；2Institute of Animal Husbandry and Veterinary Sciences,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China

Received:1900-01-01 Revised:1900-01-01 Online:2012-01-24 Published:2012-01-24

摘要/Abstract

摘要：

鸭坦布苏病毒(Duck Tembusu virus,DTMUV)是一种新发现可引起鸭产蛋下降的新型黄病毒,建立一种病原学检测方法对于疫病的诊断和流行病学研究具有重要意义。在对DTMUV序列的比对分析的基础上,设计了1对特异性引物,通过优化PCR反应条件,建立了DTMUV的一步法RTPCR检测方法。该方法具有特异、快速、敏感、准确等特点,可以检出182个TCID50的病毒核酸。对2010年至2011年6月份前的106份产蛋下降鸭群临床病料进行了检测,结果显示样品阳性率为46.2%,表明DTMUV在浙江及其周边省份的产蛋鸭群具有较高的感染率。

关键词: 鸭坦布苏病毒, 一步法RTPCR, 临床检测

Abstract: Duck Tembusu virus (DTMUV) is a new flavivirus that can cause Duck hemorrhagic ovaritis, a duck infectious disease with eggdrop. It is important to establish a pathogen detection assay for diagnosis and epidemiological studies. A pair of primers was designed based on the analysis of TMUV sequences. The onestep RTPCR method had been successfully developed for detection of DTMUV through optimization of viral RNA extraction and RTPCR assay. The results showed that 182 TCID50 of virus could be detected in two hours. The rate of positivity in 106 clinically suspected tissues and organs of diseased duck subjected to detection by RTPCR was 46.2%. The results indicated that DTMUV is highly prevalent in Zhejiang and its neighboring provinces.

Key words: Duck Tembusu virus, onestep RTPCR, clinical detection

张帅;云涛;叶伟成;邓晓辉;崔言顺;*;华炯钢;张存;*;张燕 . 鸭坦布苏病毒一步法RTPCR检测方法的建立和应用[J]. , 2012, 24(1): 0-40.

ZHANG Shuai;YUN Tao;YE Weicheng;DENG Xiaohui;CUI Yanshun;*;HUA Jionggang;ZHANG Cun;*;ZHANG Yan . Development and application of one step RTPCR technique for detection of Duck Tembusu virus Abstract: Key words: [J]. , 2012, 24(1): 0-40.

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鸭坦布苏病毒一步法RTPCR检测方法的建立和应用

Development and application of one step RTPCR technique for detection of Duck Tembusu virus Abstract: Key words:

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