›› 2012, Vol. 24 ›› Issue (2): 0-246.

• 园艺科学 •    

天台乌药的组织培养与快速繁殖

陈珍1, 孙骏威2, 许海丹3, 钱宝英1, 鲍思伟1


  

  1. 1台州学院 生命科学学院, 浙江 临海 317000; 2中国计量学院 生命科学学院, 浙江 杭州 310018;3台州学院 医药化工学院,浙江 临海 317000
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2012-03-25 发布日期:2012-03-25

Tissue culture and rapid propagation of Lindera aggregate (Sims) Kosterm

CHEN Zhen;SUN Jun-wei;XU Hai-dan;QIAN Bao-ying;BAO Si-wei   

  1. 1College of Life Sciences, Taizhou University, Linhai 317000, China; 2College of Life Sciences, China Jiliang University, Hangzhou 310018, China;3College of Pharmaceutical and Chemical Engineering, Taizhou University, Linhai 317000, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2012-03-25 Published:2012-03-25

摘要:

以药用植物天台乌药当年生嫩茎为外植体,新芽萌动后取材,研究不同培养基对其组织培养和植株再生的影响。结果表明,最适的不定芽增殖培养基为 MS+6-BA 2.0~3.5 mg·L-1+NAA 0.2 mg·L-1+Na2S2O31.25%(蔗糖3%),最佳的生根培养基为1/4 MS+IBA 1.0 mg·L-1(不加肌醇,蔗糖2%)。

关键词: 乌药(Lindera aggregate (Sims) Kosterm), 组织培养, 快速繁殖

Abstract: The effects of different culture media on tissue culture and plant regeneration of medical plant Lindera aggregate (Sims) Kosterm were investigated with the explants of young stems with buds sprouting. The results showed that the appropriate culture medium for adventitious buds multiplication was MS basal culture medium supplemented with 6-BA 2.0~3.5 mg·L-1, NAA 0.2 mg·L-1 and Na2S2O31.25% (3% sucrose). The most suitable medium for root regeneration was 1/4 MS culture medium added with IBA 1.0 mg·L-1(2% sucrose, without inositol).

Key words: Lindera aggregate (Sims) Kosterm, tissue culture, rapid propagation