转Cry1Ab水稻纯合体快速准确的PCR鉴定方法

›› 2012, Vol. 24 ›› Issue (4): 0-553.

• 论文 •

转Cry1Ab水稻纯合体快速准确的PCR鉴定方法

张焕春1,2,汪小福2,李玥莹1,陈笑芸2,缪青梅2,徐俊锋2,*

1沈阳师范大学化学与生命科学学院,辽宁沈阳 110034,2浙江省农业科学院农产品质量标准研究所,浙江杭州 310021

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2012-07-25 发布日期:2012-07-25

A rapid and accurate PCR method for homozygous lines screening for genetically modified rice containing Cry1Ab

ZHANG Huan-chun;WANG Xiao-fu;LI Yue-ying;CHEN Xiao-yun;MIAO Qing-mei;XU Jun-feng;*

1College of Chemistry and Life Science, Shenyang Normal University, Shenyang 110034, China; 2Institute of Quality and Standard for Agricultural Products, Zhejiang Academy of Agricultural Sciences,Hangzhou 310021, China

Received:1900-01-01 Revised:1900-01-01 Online:2012-07-25 Published:2012-07-25

摘要/Abstract

摘要： 转Cry1Ab基因水稻Bt22为一种新型的转基因抗虫水稻,本研究介绍了一种筛选纯合转基因植株Bt22的PCR方法。根据外源序列在Bt22中插入位点的两侧旁邻序列设计引物,建立了转基因抗虫水稻Bt22品系特异的定性PCR检测方法,该方法灵敏度可达0.01 ng,在此基础上组合各引物建立了筛选转基因水稻Bt22纯合体的多重PCR方法,结果表明该方法简单高效,便于操作。

关键词: 转基因水稻, 特异性定性PCR, 多重PCR, 纯合性检测

Abstract: Bt22 is a new type of rice that has been genetically modified to express Cry1Ab protein, we explored a new method based on PCR to screen the homozygous transgenic offspring. The primers were designed on the flanking sequence of the exogenous gene and the specificity and sensitivity of the primers were tested by experiment. Then we established multiPCR methods to screen homozygous plant from numerous transgenic offspring. Our results showed that this method is simple, efficient and convenient．

Key words: genetically modified rice, eventspecific qualitative PCR, multiplex PCR, zygosity testing

张焕春;汪小福;李玥莹;陈笑芸;缪青梅;徐俊锋;*. 转Cry1Ab水稻纯合体快速准确的PCR鉴定方法[J]. , 2012, 24(4): 0-553.

ZHANG Huan-chun;WANG Xiao-fu;LI Yue-ying;CHEN Xiao-yun;MIAO Qing-mei;XU Jun-feng;* . A rapid and accurate PCR method for homozygous lines screening for genetically modified rice containing Cry1Ab[J]. , 2012, 24(4): 0-553.

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转Cry1Ab水稻纯合体快速准确的PCR鉴定方法

A rapid and accurate PCR method for homozygous lines screening for genetically modified rice containing Cry1Ab

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