脂质体对草鱼出血病核酸疫苗免疫效果的影响

›› 2012, Vol. 24 ›› Issue (4): 0-558.

• 论文 •

脂质体对草鱼出血病核酸疫苗免疫效果的影响

徐诗英1,邹勇2,3,王习达2,刘林3,杨家新1,陈辉2,*

1南京师范大学生命科学学院,江苏南京 210046；2江苏省水生动物疫病预防控制中心江苏省水生动物防疫检疫研究重点实验室,江苏南京 210036；3苏州大学基础医学与生物科学学院,江苏苏州 215123

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2012-07-25 发布日期:2012-07-25

Effects of liposome on immune efficacy of hemorrhage disease of grass carp DNA vaccine

XU Shi-ying;ZOU Yong;WANG Xi-da;LIU Lin;YANG Jia-xin;CHEN Hui;*

1 College of Life Sciences, Nanjing Normal University, Nanjing 210046, China; 2 Jiangsu Provincial Key Laboratory for Aquatic Animal Epidemic Prevention and Quarantine, Jiangsu Center for Control and Prevention of Aquatic Animal Infectious Disease, Nanjing 210036, China; 3School of Biology and Basic Medical Sciences, Soochow University, Suzhou 215123, China

Received:1900-01-01 Revised:1900-01-01 Online:2012-07-25 Published:2012-07-25

摘要/Abstract

摘要： 将已构建完成的草鱼出血病核酸疫苗pFastBac-β-VP71-VP72以30 μg的剂量,经Lipotap脂质体包裹后,背部肌肉注射草鱼体内,定期通过RTPCR测定目的基因转录水平、间接凝集反应测定抗体水平,并通过与相应的重组质粒组、空载体组和对照组进行比较,来评价脂质体对核酸疫苗免疫保护效果的影响。结果显示,脂质体组至第70天仍能检测到目的基因的转录,且抗体效价明显高于其他各组,说明脂质体可显著增强该核酸疫苗的免疫活性,可作为一种有应用前景的核酸疫苗佐剂。

关键词: 草鱼出血病, 核酸疫苗, 脂质体, 免疫效果

Abstract: Recombinant plasmids pFastBac-β-VP71-VP72, which were used as DNA vaccines based on 30 μg dose, were injected into grass carps by dorsal muscles after being enwrapped by liposome. Transcription situation and antibody level were tested respectively on a regular basis from RTPCR and indirect agglutination reaction for evaluating the role of liposome in comparison with no liposome groups, pFastBacTMDual group and control. The results indicated that transcription of target gene was still in process on the 70th day in liposome group, and antibody level was higher than no liposome groups. Therefore, liposome could promote the immune effect of DNA vaccine and should be further studied and put into practice as a kind of DNA vaccine adjuvant．

Key words: hemorrhage disease of grass carp, DNA vaccine, liposome, immune efficacy

徐诗英;邹勇;王习达;刘林;杨家新;陈辉;*. 脂质体对草鱼出血病核酸疫苗免疫效果的影响[J]. , 2012, 24(4): 0-558.

XU Shi-ying;ZOU Yong;WANG Xi-da;LIU Lin;YANG Jia-xin;CHEN Hui;*. Effects of liposome on immune efficacy of hemorrhage disease of grass carp DNA vaccine[J]. , 2012, 24(4): 0-558.

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脂质体对草鱼出血病核酸疫苗免疫效果的影响

Effects of liposome on immune efficacy of hemorrhage disease of grass carp DNA vaccine

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