关键词: 绵羊卵巢, 运输温度, 保存温度, 卵母细胞, 发育能力

Abstract: The effect of transport and storage temperature and time on parthenogenetically development ability of sheep oocyte was investigated. Ovary were collected and randomly placed in physiological saline at 10-15℃, 20-25℃ and 30-35℃ and transported to the laboratory to culture. They were divided into three groups randomly in optimal transport temperature, respectively kept in 4℃, 14-18℃ and 25-30℃ physiological saline, then oocytes were matured and parthenogenetic activated after 15-17 h. The results showed that the optimal transport temperature of ovaries was 20-25℃, the highest mature rates was 67.44% and blastocysts rates was 35.93%. When the sheep ovaries were kept in 14-18℃ for 15-17 h, it was found that the corresponding maturation rate(61.81%) and blastocysts rates(29.03%) of oocytes had no significant difference (P>0.05). the oocytes mature rates (41.90%, 18.40%) and the rates of cleavage (9.09%, 13.04%) were significantly lower and did not grow to blastocysts when they were kept in 4℃ or 25-30℃. It can be concluded that the optimal transport temperature of the sheep ovaries was 20-25℃, and oocytes of the sheep ovary in vitro had a good growth at 14-18℃ overnight．

Key words: sheep ovary, storage temperature, transport temperature, oocyte, developmental competence