›› 2012, Vol. 24 ›› Issue (4): 0-629.

• 论文 •    

生防菌株MT-06对黄瓜白粉病的防治及其定殖力测定

彭志荣1,2,毛雪琴2,#,姜华2,柴荣耀2,张震2,王艳丽2,王教瑜2,邱海萍2,孙国昌2,*   

  1. 1浙江师范大学化学与生命科学学院,浙江 金华321004;2浙江省植物有害生物防控重点实验室—省部共建国家重点实验室培育基地, 浙江省农业科学院植物保护与微生物研究所,浙江 杭州 310021
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2012-07-25 发布日期:2012-07-25

Evaluation of biological control strain MT06 for managing cucumber powdery mildew by pot tests and its colonization measurement on cucumber

PENG Zhirong;MAO Xueqin;#;JIANG Hua;CHAI Rongyao;ZHANG Zhen;WANG Yanli;WANG Jiaoyu;QIU Haiping;SUN Guochang;*   

  1. 1College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, China; 2.State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Institute of Plant Protection Microbiology,Zhejiang Academy of Agricultural Sciences, Hangzhou 310021,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2012-07-25 Published:2012-07-25

摘要: 不同MT-06发酵液、孢子液和菌液防治黄瓜白粉病的盆栽试验研究发现:不同浓度梯度MT-06制剂对黄瓜白粉病都有抑制作用。MT-06菌液10倍稀释液处理3 d后的保护效果和治疗效果分别达到87.86%和84.89%,与对照三唑酮的处理效果相当,但处理11 d后MT-06菌液10倍稀释液的治疗效果显著高于三唑酮处理(P<0.05),其他处理防治效果都未能达到三唑酮的防治效果。MT-06菌液和孢子液表现防效较MT-06发酵液持久。MT-06定殖试验表明:叶柄上的定殖率仅1×105个·mL-1 MT-06孢子液为85%,其余孢子液及菌液处理定殖率均为100%;MT-06在叶片上定殖率较低,MT-06菌液10倍稀释液的定殖率最高为40%。分析认为MT-06防效的保持性较好可能与其能定殖于植物上有关。

关键词: 黄蓝状菌, 黄瓜白粉病, MT-06, 生防

Abstract: The evaluation was carried out to determine the ability of Talaromyces flavus, MT-06based products in management of cucumber powdery mildew, caused by Sphaerotheca fuliginea. The results showed that the effectiveness of strain MT-06 was dependent on its concentration in pot tests. In particular, 10fold dilution of mycelium mixed liquid have the best effect, with the preventive effects of 87.86% and the curative effects of 84.89% compared to the control after spraying 11 days, while the effect of the other treatments of strain MT-06 are similar to the chemical fungicide triadimefon. The effect of fermented liquid decreased with the prolonging of time; however, the effect of mycelium mixed liquid and spore suspension kept stable for a long time. The colonization test of strain MT-06 on cucumber plants showed that petiole colonization rate is higher than that of the leaves. The petiole colonization rate of mycelium mixed liquid and spore suspension was 100%, except when sprayed the spore suspension at the concentration of 1×105 mL-1 was 85%, while the highest colonization rate on cucumber leaves was 40%. Therefore, the biocontrol efficiency of strain MT06 may be partially attributed to the ability of colonization on cucumber plants. Overall, these results showed that strain MT-06 played an important role in managing powdery mildew on cucumber plants.

Key words: Talaromyces flavus, cucumber powdery mildew, MT-06, colonization