›› 2012, Vol. 24 ›› Issue (4): 0-720.

• 论文 •    

一种基于模板DNA的PCR扩增难易预测方法

范秀静,李楠,李春   

  1. 渤海大学 数理学院, 辽宁 锦州 121000
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2012-07-25 发布日期:2012-07-25

A predictive method for polymerase chain reactions based on the template DNA

FAN Xiujing;LI Nan;LI Chun   

  1. College of Mathematics and Physics, Bohai University, Jinzhou 121000, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2012-07-25 Published:2012-07-25

摘要: 借助赋予四种碱基A,G,C,T四个三维空间中的点(0,0,1),(0,1,0),(-1,-1,0),(1,0,0),将一条DNA序列转化为一个二十四维向量。在此基础上利用Fisher线性判别法对人类基因组中189条基因片段进行PCR扩增难易预测,识别率达到了90%。

关键词: 模板DNA, 24维向量, 聚合酶链式反应(PCR)

Abstract: By means of assigning (0,0,1),(0,1,0),(-1,-1,0),(1,0,0) to the four bases A, G, C and T, a DNA sequence is transformed into a 24dimensional vector. Based on this and the Fisher linear discriminant algorithm, we proposed a computational method to predict success for PCR. Our report is based on 189 amplicons randomly selected from human exons, and our method achieves an accuracy of 90%.

Key words: template DNA, 24dimensional vector, polymerase chain reactions (PCR)