关键词: 单倍体诱导系, 染色体加倍, 气孔密度, DH育种

Abstract:

Eight different genotype female parent materials were induced by three haploid inducers （Stock 6， Gaoyou 1 and MT041） to test their ability of producing haploid through grain color， chromosome number of root tip， stomatal density and field planting performance， and colchicine was used for doubling. The results showed that haploid induction rates of different inducers differed greatly. The haploid induction rate of MT041 was 4.17%， which was higher than the other two inducers. The female genotypes showed significant difference in induction rates. The haploid induction rates of the waxy and ordinary materials were significantly higher than sweet materials. The sweetwaxy corn hybrids were beneficial to increase the sweet corn haploids. The doubling effect of 0.06% colchicine solution was significantly higher than 0.02% colchicine solution by rootsoaking， while seedling percentage in high concentration colchicine treatment was not high. Chromosome doubling is still a bottleneck restricting widespread application of the DH breeding techniques．

Key words: haploid inducer, chromosomedoubling, stomatal density, DH breeding