›› 2012, Vol. 24 ›› Issue (6): 0-1078.

• 论文 •    

植物组培产业化生产中污染防控技术研究

方丽1,汪一婷2,吕永平2,Kumar2,陈剑平2,王汉荣1,*   

  1. 1浙江省农业科学院 植物保护与微生物研究所,浙江 杭州 310021;2浙江省农业科学院 病毒学与生物技术研究所,浙江 杭州 310021
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2012-11-25 发布日期:2012-11-25

Prevention and control technology of pollution in industrial tissue culture

FANG Li;WANG Yiting;LYU Yongping;Kumar;CHEN Jianping;WANG Hanrong;*   

  1. 1Institute of Plant Protection and microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China; 2Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2012-11-25 Published:2012-11-25

摘要: 对浙江省植物组培产业生产中心的主要污染微生物的防控技术进行了研究,从11个杀菌剂和消毒剂中筛选出了对污染细菌德氏假单胞菌(Pseudomonas delafieldii)、丁香假单胞菌(Pseudomonas syringae)、蜡状芽胞菌(Bacillus cereus)和污染真菌指状青霉(Penicillum digitatum)、黑曲霉(Aspergillus niger)预防效果好,且对组培植物安全的药剂为乙蒜素和链·土霉素。乙蒜素可以在培养基分装前加入,与培养基同时灭菌使用;而链·土霉素在培养基使用前加入可有效预防污染物生长,该药剂对组培器皿和环境也有较好的消毒效果。

关键词: 植物组织培养, 污染防控技术, 乙蒜素, 链·土霉素

Abstract: Prevention and control technology of pollution in industrial tissue culture had been carried out in Hangzhou and Ningbo, the main tissue culture industry production center in Zhejiang province Two medicines ethylicin and streptomycinterramycin had been screened out for their good control effects on common pollution microflora such as Pseudomonas delafieldii, Pseudomonas syringae, Bacillus cereus, Penicillum digitatum and Aspergillus niger, from 11 germicides and disinfectants And they showed safety to tissue culture seedling and easy to use Ethylicin could be added into culture medium before culture autoclaving for its high temperature resistance, while streptomycinterramycin could only be used in the sterilized culture medium to prevent the growth of contaminants The medicament also had a good disinfection effect for the tissue culture vessel or for the environment.

Key words: plant tissue culture, pollution control technology, ethylicin, streptomycinterramycin