关键词: 转基因检测, 热加工, 转基因水稻

Abstract: In order to study the effect of heat processing on transgenic rice detection， four artificial heat process including poaching， hyperthermia， high temperature drying and microwave were employed to deal with three transgenic rice varieties including KF6， KMD1 and TT51-1，which were three prevalent transgenic rice varieties in China. According to the national detection standard， four parameters were selected to detect by PCR method. These results showed that whether the endogenous gene or the inserted exogenous genes were susceptible to the heat process， indicating that the current standard for transgenic rice processed products detection has some limitations. We designed new primers based on the Bt sequence to test the heat processed samples， and the amplification result was better than the standard primers.

Key words: transgenic detection, heat process, transgenic rice