›› 2009, Vol. 21 ›› Issue (4): 0-334.

• 论文 •    

急性热应激对蛋鸭内脏组织HSP70表达的影响

邹丽丽1,2,陶争荣 2,沈军达 2,任晋东 2,3,石放雄 1,*,卢立志 2,*   

  1. 1 南京农业大学动物科技学院,江苏南京210095; 2 浙江省农业科学院畜牧兽医研究所,浙江杭州310021;3 西北农林科技大学动物科技学院,陕西杨凌71002
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2009-07-25 发布日期:2009-07-25

Efects of acute heat stress on expressional patterns of the heat shock protein 7O in the duck internal organs

ZOU Li—li;TAO Zheng-rong;SHEN Jun-da;REN Jin—dong;;SHI Fang-xiong;*;LU Li-zhi;*   

  1. 1 College ofAnimal Science and Technology,Nanjing Agricultural University,Nanjing 210095,China;2 Institute of Animal Husbandry and Veterinary Medicine,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China;3 College of Animal Science and Technology,Northwest University ofagriculture and Forestry Sci-technology,Yangling 710021,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2009-07-25 Published:2009-07-25

摘要: 选取20只处于产蛋高峰期的蛋鸭,急性热应激处理后,用RT-PCR和Western-blot研究蛋鸭各内脏器官内热休克蛋白70(heat shock protein 70,HSP-/0)的表达情况。结果显示:急性热应激后内脏组织肝脏、心脏和卵巢中的HSP70 mRNA和蛋白水平的表达量无明显变化,肾脏内mRNA水平的表达量显著下降,而脾脏内mRNA和蛋白水平的表达量呈短暂下降后又恢复到原来水平。结果表明当蛋鸭遭受急性热应激时内脏器官呈现特异性反应。

关键词: 急性热应激, HSP70, 蛋鸭

Abstract: Twenty laying ducks were expo~ under acute heat stress during the peak period of egg-laying,and the expressional patterns of heat shock protein 70(HSP70)in the intemal organs ofthese ducks were examined using RT—PCR and
western-blot.The Iesult indicated that the mRNA and protein levels ofHSP70 in heart.1iver and ovary were not changed after acute heat stres,while the mRNA levels of HSP70 were reduced in the kidney and the mRNA and protein levels of HSPT0 in the spleen were transitorily decreased but recovered after 7 h of acute heat stress.In conclusion,the expression of HSPT0 in the internal organs of laying ducks showed specific responses under acute heat stress.

Key words: acute heat stress, HSP70, laying duck