关键词: 柃木, 组织培养, 快速繁殖, 增殖

Abstract: The cut fresh branches of Eurya japonica in Zhejiang are processed as a kind of traditional forest products to be exported to Japan. In order to establish a rapid propagation system on shoot tip culture of E. japonica, the effects of different sterilization time of HgCl₂ on polluted rate of explants, the effects of different 6-BA concentrations on shoot subculture and multiplication culture, the effects of different IBA concentrations, sucrose concentrations and rooting methods on rooting rate were studied. The results showed that the appropriate conditions for tissue culture and rapid propagation of E. japonica were as follows: 25 min shoot surface-sterilization with 0.2% HgCl₂, WPM basal medium with 0.5 mg·L^-1 6-BA, 0.05 mg·L^-1 NAA, 0.2 mg·L^-1 GA, 20 g·L^-1 sucrose in proliferation culture, 0.5 mg·L^-1 IBA, 30 g·L^-1 sucrose and two-step rooting method in rooting culture. Under this condition, bud-induction rate, multiplication coefficient, rooting rate and transplant survival rate of tissue culture seedlings could reach 100%, 2.56-4.85 per time, 98% and >95% respectively．

Key words: Eurya japonica Thunb, tissue culture, rapid propagation, proliferation