›› 2013, Vol. 25 ›› Issue (2): 0-283.

• 论文 •    

抗菌肽Caseicin A氨基酸排列顺序改变对其抑菌活性的影响

谢秀枝1,刘丽华1,皮雄娥2,刘伟2,陈荣庆3,王欣2,*   

  1. 1内蒙古工业大学 化工学院,内蒙古 呼和浩特010051;2浙江省农业科学院 植物保护与微生物研究所,浙江 杭州310021;3浙江省师范大学 化学与生命科学学院,浙江 金华321004
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2013-03-25 发布日期:2013-03-25

Effect of caseicin As amino acid sequence alteration on its antimicrobial activity

XIE Xiu-zhi;LIU Li-hua ;PI Xiong-e;LIU Wei;CHEN Rong-qing;WANG Xin;*   

  1. 1 College of Chemical Engineering, Inner Mongolia University of Technology, Hohhot 010051, China;2 Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China; 3College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2013-03-25 Published:2013-03-25

摘要: Caseicin A是一种细菌发酵生产酪蛋白酸钠时产生的次级代谢产物,目前已有报道证明其对革兰氏阴性和阳性致病菌具有一定的抑菌活性。本试验通过对Caseicin A的氨基酸进行重排,得到了比原始抗菌肽抑菌活性更高的突变体。结果表明,改变抗菌肽氨基酸的排列顺序会引起其抗菌活性的变化,但对于不同的受试菌具有不同的影响。

关键词: 酪蛋白, Caseicin A, 抑菌活性, 最小抑菌浓度

Abstract: Caseicin A is an antimicrobial peptide (ABP) generated through the bacterial fermentation of sodium caseinate, and on the basis of previous studies, it is active against many Gram-negative pathogens and Gram-positive organisms. In order to obtain more active ABP, the amino acid sequence of Caseicin A was rearranged. Our results showed that the changes of amino acid sequence would cause the alteration of antimicrobial activity, but the influence was different on different tested bacteria.

Key words: casein, caseicin A, antimicrobial activity, minimum antibacterial concentrations