›› 2013, Vol. 25 ›› Issue (5): 0-1055.

• 论文 •    

白术真菌病害的分离鉴定

檀国印,杨志玲*,袁志林,杨旭
  

  1. 中国林业科学研究院 亚热带林业研究所,浙江 富阳 311400
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2013-09-25 发布日期:2013-09-25

Isolation and identification of fungal pathogens of Atractylodes macrocephala

TAN Guo-yin;YANG Zhi-ling*;YUAN Zhi-lin;YANG Xyu   

  1. Institute of Subtropical Forestry, Chinese Academy of Forestry, Fuyang 311400, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2013-09-25 Published:2013-09-25

摘要: 2011年6— 8月份在浙江磐安地区采集到白术病害根、茎和叶片样本,经过室内分离试验得到4个根茎真菌分离物(分别记为R1~R4)与8个叶片真菌分离物(分别记为L1~L8)。依据科赫法则进行致病性测定,R1,R2为白术茎腐病病原菌,L3为白术叶斑病病原菌。通过形态学观察及分子鉴定方法确定R1为尖孢镰刀菌Fusarium oxysporum,R2为半裸镰刀菌Fusarium incarnatum,L3为长柄链格孢菌Alternaria longipes。

关键词: 白术, 真菌病害, 尖孢镰刀菌, 半裸镰刀菌, 长柄链格孢菌

Abstract: Diseased Atractylodes macrocephala were sampled in Panan, Zhejiang Province during June and August, 2011. Several fungus, 4 from stems (R1~R4) and 8 from leaves (L1~L8), were isolated. The results determined by Kochs postulates indicated that R1 and R2 could cause stem\|rot disease, while L3 could cause leaf spot on Atractylodes macrocephala. Through morphological and molecular identification, R1, R2 and L3 were Fusarium oxysporum, Fusarium incarnatum and Alternaria longipes, respectively.

Key words: Atractylodes macrocephala, fungal diseases, Fusarium oxysporum, Fusarium incarnatum, Alternaria longipes