芹菜茎基腐病病原菌鉴定、消长动态调查与防治药剂筛选

doi:10.3969/j.issn.1004-1524.2021.04.11

浙江农业学报 ›› 2021, Vol. 33 ›› Issue (4): 661-669.DOI: 10.3969/j.issn.1004-1524.2021.04.11

芹菜茎基腐病病原菌鉴定、消长动态调查与防治药剂筛选

王国荣¹(), 冯晓晓², 吴慧明³, 曹婷婷⁴, 李倩⁵, 郑永利⁶^,^*()

1.浙江省杭州市萧山区农业技术推广中心,浙江杭州311203
2.浙江大学农业试验站,浙江杭州310058
3.浙江农林大学农业与食品科学学院,浙江杭州311300
4.浙江省植保检疫与农药管理总站,浙江杭州310020
5.中国计量大学生命科学学院,浙江杭州 310018
6.浙江省农产品质量安全中心,浙江杭州310003

收稿日期:2020-07-14 出版日期:2021-04-25 发布日期:2021-04-25
通讯作者: 郑永利
作者简介:*郑永利,E-mail:yonglizheng@yeah.net
王国荣(1970—),男,浙江萧山人,农业推广硕士,高级农艺师,长期从事植保技术推广工作。E-mail:595902466@qq.com
基金资助:
浙江省“三农六方”科技协作项目(CTZB-F180706LWZ-SNY1);浙江省重点研发计划(2020C02027)

Identification of causal agent and dynamics survey of celery stalk basal rot and screening of fungicides

WANG Guorong¹(), FENG Xiaoxiao², WU Huiming³, CAO Tingting⁴, LI Qian⁵, ZHENG Yongli⁶^,^*()

1. Xiaoshan Agricultural Technology Extension Center, Hangzhou 311203, China
2. Agricultural Experiment Station, Zhejiang University, Hangzhou 310058, China
3. College of Agriculture and Food Science, Zhejiang A & F University, Hangzhou 311300, China
4. Zhejiang Plant Protection Quarantine and Pesticide Management Station, Hangzhou 310020, China
5. College of Life Sciences, China Jiliang University, Hangzhou 310018, China
6. Zhejiang Agricultural Products Quality and Safety Center, Hangzhou 310003, China

Received:2020-07-14 Online:2021-04-25 Published:2021-04-25
Contact: ZHENG Yongli

摘要/Abstract

摘要：

为探明芹菜茎基腐病科学防控技术,于2017—2018年在浙江省杭州市系统观测了芹菜茎基腐病田间病情自然消长动态;采取组织分离、形态和系统发育分析、菌丝悬浮液接种等方法对病原菌进行了分离鉴定和致病性测定;采用菌丝生长速率法测定了29种常用杀菌剂对芹菜茎基腐病病原菌的抑制率,并开展了相关田间药效试验。结果表明,芹菜茎基腐病在芹菜上普遍发生,6—9月是该病的发病高峰期;引起芹菜茎基腐病的病原菌为尖孢镰刀菌,甲基硫菌灵和戊唑醇及其复配制剂对芹菜茎基腐病菌的菌丝生长抑制效果最佳,75%肟菌·戊唑醇WDG或70%甲基硫菌灵WP对芹菜茎基腐病的田间防效较好。

关键词: 芹菜茎基腐病, 尖孢镰刀菌, 杀菌剂

Abstract:

In order to characterize the causal agent and occurrence of celery stalk basal rot in Hangzhou, Zhejiang province and effective fungicides, the dynamic survey of celery stalk rot in field was investigated in 2017-2018, the pathogen was isolated and identified by tissue separation, morphological and phylogenetic analysis and its pathogenicity was determined by mycelial suspension inoculation, the inhibition of 29 common fungicides were determined by mycelial growth rate method, and field control experiment were conducted as well. The results showed that frequent occurrence of celery stalk basal rot was during June to September. Fusarium oxysporum was the pathogenic fungus that caused celery stalk basal rot. In vitro screening test showed that thiophanate-methyl and tebuconazoles fungicides had the best inhibitory effect on mycelial growth of pathogenic F. oxysporum, and field application of 75% trifloxystrobin·tebuconazole WDG and 70% thiophanate-methyl WP had better control effect on celery stalk basal rot.

Key words: celery stalk basal rot, Fusarium oxysporum, fungicide

中图分类号:

S436.36

王国荣, 冯晓晓, 吴慧明, 曹婷婷, 李倩, 郑永利. 芹菜茎基腐病病原菌鉴定、消长动态调查与防治药剂筛选[J]. 浙江农业学报, 2021, 33(4): 661-669.

WANG Guorong, FENG Xiaoxiao, WU Huiming, CAO Tingting, LI Qian, ZHENG Yongli. Identification of causal agent and dynamics survey of celery stalk basal rot and screening of fungicides[J]. Acta Agriculturae Zhejiangensis, 2021, 33(4): 661-669.

图/表 9

参考文献 10

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处理 Treatments	药剂 Fungicide	使用方法 Methods of use
T1	70%甲基硫菌灵可湿性粉剂800倍液 70% Thiophanate methyl WP 800×	移栽前浸根(1)+移栽缓苗后粗喷雾(2)+封行前粗喷雾 Dipping root before transplanting (1)+Spraying on stem base after reviving (2)+Spraying on stem base before sealing
T2	70%甲基硫菌灵可湿性粉剂800倍液 70% Thiophanate methyl WP 800×	移栽缓苗后粗喷雾+封行前粗喷雾 Spraying at stem base after reviving+Spraying at stem base before sealing
T3	75%肟菌·戊唑醇水分散粒剂2000倍液 75% Trifloxysporin·Tebuconazole WDG 2000×	移栽前浸根+移栽缓苗后粗喷雾+封行前粗喷雾 Dipping root before transplanting+Spraying on stem base after reviving+Spraying on stem base before sealing
T4	75%肟菌·戊唑醇水分散粒剂2000倍液 75% Trifloxysporin·Tebuconazole WDG 2000×	移栽缓苗后粗喷雾+封行前粗喷雾 Spraying at stem base after reviving+Spraying at stem base before sealing
T5	Water	喷雾 Spraying

处理 Treatments	药剂 Fungicide	使用方法 Methods of use
T1	70%甲基硫菌灵可湿性粉剂800倍液 70% Thiophanate methyl WP 800×	移栽前浸根(1)+移栽缓苗后粗喷雾(2)+封行前粗喷雾 Dipping root before transplanting (1)+Spraying on stem base after reviving (2)+Spraying on stem base before sealing
T2	70%甲基硫菌灵可湿性粉剂800倍液 70% Thiophanate methyl WP 800×	移栽缓苗后粗喷雾+封行前粗喷雾 Spraying at stem base after reviving+Spraying at stem base before sealing
T3	75%肟菌·戊唑醇水分散粒剂2000倍液 75% Trifloxysporin·Tebuconazole WDG 2000×	移栽前浸根+移栽缓苗后粗喷雾+封行前粗喷雾 Dipping root before transplanting+Spraying on stem base after reviving+Spraying on stem base before sealing
T4	75%肟菌·戊唑醇水分散粒剂2000倍液 75% Trifloxysporin·Tebuconazole WDG 2000×	移栽缓苗后粗喷雾+封行前粗喷雾 Spraying at stem base after reviving+Spraying at stem base before sealing
T5	Water	喷雾 Spraying

供试药剂 Fungicide	生产企业 Manufacture	试验浓度 Concentration/ (mg·L^-1)	抑制率 Inhibition/%	敏感性评价 Sensitivity evaluation
430 g·L^-1戊唑醇SC 430 g·L^-1 Tebuconazole SC	拜耳 Bayer	1.7	100 a	强 Hypersensitive
40%丙硫菌唑·戊唑醇SC 40%Prothioconazole·Tebuconazole SC	海利尔 Waier	13.3	100 a	强Hypersensitive
70%甲基硫菌灵WP 70% Thiophanate methyl WP	日友 Japfou	17.5	100 a	强Hypersensitive
30%唑醚·戊唑醇SC 30% Pyraclostrobin·Tebuconazole SC	明德立达 Pilar	9.2	99.25±0.75 a	强Hypersensitive
20%氰烯·己唑醇SC 20%Phenamacril· Hexaconazole SC	上格 Sunger	18.7	95.80±0.37 ab	强Hypersensitive
75%肟菌·戊唑醇WDG 75%Trifloxazole·Tebuconazole WDG	拜耳 Bayer	10.0	95.33±0.94 a	强Hypersensitive
40%氟菌唑WP 40% Triflumizole WP	恒田生物 Hengtian Biological	4.3	90.40±0.75 bc	强Hypersensitive
125 g·L^-1氟环唑SC 125 g·L^-1Epoxiconazole SC	巴斯夫 BASF	2.5	87.80±0.49 bcd	强Hypersensitive
35%氟菌·戊唑醇SC 35% Fluopyram·Tebuconazole SC	拜耳 Bayer	3.5	86.40±1.43 cd	强Hypersensitive
43%氟菌·肟菌酯SC 43% Fluopyram·Trifloxazole SC	拜耳 Bayer	4.3	78.60±0.81 de	中Sensitive
12%苯甲·氟酰胺SC 12%Difenoconazole· Fluxapyroxad SC	巴斯夫 BASF	5.6	76.60±0.68 ef	中Sensitive
19%啶氧·丙环唑SC 19%Picoxystrobin· Propiconazol SC	杜邦 DuPont	11.1	71.60±0.68 efg	中Sensitive
10%苯醚甲环唑WDG 10% Difenoconazole WDG	先正达 Syngenta	6.7	70.84±6.08 b	中Sensitive
25%氰烯菌酯SC 25% Phenamacril SC	苏研 SUYAN	2.5	69.53±2.54 bc	中Sensitive
供试药剂 Fungicide	生产企业 Manufacture	试验浓度 Concentration/ (mg·L^-1)	抑制率 Inhibition/%	敏感性评价 Sensitivity evaluation
60%唑醚·代森联WDG 60%Pyraclostrobin·Metiram WDG	巴斯夫 BASF	48.0	68.00±0.32 fgh	中Sensitive
30%己唑醇SC 30% Hexaconazole SC	上格 Sunger	1.0	65.80±0.58 gh	中Sensitive
400 g·L^-1氟唑菌酰羟胺·咯菌腈SC 400 g·L^-1 Pydiflumetofen·Fludioxonil SC	先正达 Syngenta	5.0	63.36±1.55 bc	中Sensitive
16%二氰·吡唑酯WDG 16%Dithianon·Pyraclostrobin WDG	巴斯夫 BASF	17.8	59.80±4.84 h	中Sensitive
30%苯甲·嘧菌酯SC 30% Difenoconazole·Azoxystrobin SC	丰乐 Fengle	10.0	56.82±2.18 c	中Sensitive
250 g·L^-1吡唑醚菌酯EC 250 g·L^-1Pyraclostrobin EC	巴斯夫 BASF	8.3	46.00±0.84 i	弱Hyposensitive
42.4%唑醚·氟酰胺SC 42.4% Pyraclostrobin·Fluxapyroxad SC	巴斯夫 BASF	8.5	45.80±1.32 i	弱Hyposensitive
4%四氟醚唑EW 4% Tetraconazole EW	意赛格 Isagro	2.7	44.60±0.75 i	弱Hyposensitive
70%代森联WDG 70% Metiram WDG	巴斯夫 BASF	77.8	42.00±1.34 ij	弱Hyposensitive
50%嘧菌环胺WDG 50% Cyprodinil WDG	先正达 Syngenta	16.0	34.40±1.91 j	弱Hyposensitive
25 g·L^-1咯菌腈FS 25 g·L^-1 Fludioxonil FS	先正达 Syngenta	24.0	21.5±3.17 d	极弱Dull
41.7%氟吡菌酰胺SC 41.7% Fluopyram SC	拜耳 Bayer	2.8	18.60±1.21 k	极弱Dull
50%烯酰吗啉WP 50% Dimethomorph WP	巴斯夫 BASF	13.3	11.80±3.22 k	极弱Dull
50%啶酰菌胺WDG 50% Boscalid WDG	巴斯夫 BASF	16.7	9.60±4.40 k	极弱Dull
15%噁霉灵AS 15% Hymexazol AS	标正 BIOGEN	3.0	-6.32±3.77 l	极弱Dull

供试药剂 Fungicide	生产企业 Manufacture	试验浓度 Concentration/ (mg·L^-1)	抑制率 Inhibition/%	敏感性评价 Sensitivity evaluation
430 g·L^-1戊唑醇SC 430 g·L^-1 Tebuconazole SC	拜耳 Bayer	1.7	100 a	强 Hypersensitive
40%丙硫菌唑·戊唑醇SC 40%Prothioconazole·Tebuconazole SC	海利尔 Waier	13.3	100 a	强Hypersensitive
70%甲基硫菌灵WP 70% Thiophanate methyl WP	日友 Japfou	17.5	100 a	强Hypersensitive
30%唑醚·戊唑醇SC 30% Pyraclostrobin·Tebuconazole SC	明德立达 Pilar	9.2	99.25±0.75 a	强Hypersensitive
20%氰烯·己唑醇SC 20%Phenamacril· Hexaconazole SC	上格 Sunger	18.7	95.80±0.37 ab	强Hypersensitive
75%肟菌·戊唑醇WDG 75%Trifloxazole·Tebuconazole WDG	拜耳 Bayer	10.0	95.33±0.94 a	强Hypersensitive
40%氟菌唑WP 40% Triflumizole WP	恒田生物 Hengtian Biological	4.3	90.40±0.75 bc	强Hypersensitive
125 g·L^-1氟环唑SC 125 g·L^-1Epoxiconazole SC	巴斯夫 BASF	2.5	87.80±0.49 bcd	强Hypersensitive
35%氟菌·戊唑醇SC 35% Fluopyram·Tebuconazole SC	拜耳 Bayer	3.5	86.40±1.43 cd	强Hypersensitive
43%氟菌·肟菌酯SC 43% Fluopyram·Trifloxazole SC	拜耳 Bayer	4.3	78.60±0.81 de	中Sensitive
12%苯甲·氟酰胺SC 12%Difenoconazole· Fluxapyroxad SC	巴斯夫 BASF	5.6	76.60±0.68 ef	中Sensitive
19%啶氧·丙环唑SC 19%Picoxystrobin· Propiconazol SC	杜邦 DuPont	11.1	71.60±0.68 efg	中Sensitive
10%苯醚甲环唑WDG 10% Difenoconazole WDG	先正达 Syngenta	6.7	70.84±6.08 b	中Sensitive
25%氰烯菌酯SC 25% Phenamacril SC	苏研 SUYAN	2.5	69.53±2.54 bc	中Sensitive
供试药剂 Fungicide	生产企业 Manufacture	试验浓度 Concentration/ (mg·L^-1)	抑制率 Inhibition/%	敏感性评价 Sensitivity evaluation
60%唑醚·代森联WDG 60%Pyraclostrobin·Metiram WDG	巴斯夫 BASF	48.0	68.00±0.32 fgh	中Sensitive
30%己唑醇SC 30% Hexaconazole SC	上格 Sunger	1.0	65.80±0.58 gh	中Sensitive
400 g·L^-1氟唑菌酰羟胺·咯菌腈SC 400 g·L^-1 Pydiflumetofen·Fludioxonil SC	先正达 Syngenta	5.0	63.36±1.55 bc	中Sensitive
16%二氰·吡唑酯WDG 16%Dithianon·Pyraclostrobin WDG	巴斯夫 BASF	17.8	59.80±4.84 h	中Sensitive
30%苯甲·嘧菌酯SC 30% Difenoconazole·Azoxystrobin SC	丰乐 Fengle	10.0	56.82±2.18 c	中Sensitive
250 g·L^-1吡唑醚菌酯EC 250 g·L^-1Pyraclostrobin EC	巴斯夫 BASF	8.3	46.00±0.84 i	弱Hyposensitive
42.4%唑醚·氟酰胺SC 42.4% Pyraclostrobin·Fluxapyroxad SC	巴斯夫 BASF	8.5	45.80±1.32 i	弱Hyposensitive
4%四氟醚唑EW 4% Tetraconazole EW	意赛格 Isagro	2.7	44.60±0.75 i	弱Hyposensitive
70%代森联WDG 70% Metiram WDG	巴斯夫 BASF	77.8	42.00±1.34 ij	弱Hyposensitive
50%嘧菌环胺WDG 50% Cyprodinil WDG	先正达 Syngenta	16.0	34.40±1.91 j	弱Hyposensitive
25 g·L^-1咯菌腈FS 25 g·L^-1 Fludioxonil FS	先正达 Syngenta	24.0	21.5±3.17 d	极弱Dull
41.7%氟吡菌酰胺SC 41.7% Fluopyram SC	拜耳 Bayer	2.8	18.60±1.21 k	极弱Dull
50%烯酰吗啉WP 50% Dimethomorph WP	巴斯夫 BASF	13.3	11.80±3.22 k	极弱Dull
50%啶酰菌胺WDG 50% Boscalid WDG	巴斯夫 BASF	16.7	9.60±4.40 k	极弱Dull
15%噁霉灵AS 15% Hymexazol AS	标正 BIOGEN	3.0	-6.32±3.77 l	极弱Dull

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芹菜茎基腐病病原菌鉴定、消长动态调查与防治药剂筛选

Identification of causal agent and dynamics survey of celery stalk basal rot and screening of fungicides

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