利用内标为基础的多重RT\|PCR技术检测草莓斑驳病毒和草莓轻型黄边病毒

›› 2014, Vol. 26 ›› Issue (1): 0-109.

• 植物保护 • 下一篇

利用内标为基础的多重RT\|PCR技术检测草莓斑驳病毒和草莓轻型黄边病毒

王红，王菁菁，张科立，成卓敏

宁波华厦现代农业研究所，浙江宁波 315000

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2014-01-25 发布日期:2014-07-09

Detection of Strawberry mottle virus and Strawberry mild yellow edge virus with multiplex RT\|PCR based on internal control

WANG Hong;WANG Jing\|jing;ZHANG Ke\|li;CHENG Zhuo\|min

Ningbo Hua Sha Agricultural Research Institute， Ningbo 315000， China

Received:1900-01-01 Revised:1900-01-01 Online:2014-01-25 Published:2014-07-09

摘要/Abstract

摘要： 草莓斑驳病毒（SMoV）和草莓轻型黄边病毒（SMYEV）分布较广，危害较重。应用CTAB法提取草莓总RNA，以优质的草莓总RNA为模板，结合扩增内标的检测体系，建立了SMoV和SMYEV的多重RT\|PCR检测体系，可以对草莓田间植株和试管苗进行快速稳定的病毒检测。

关键词: 草莓病毒, 内标, 多重RT\|PCR, 病毒检测

Abstract: Strawberry mottle virus（SMoV） and Strawberry mild yellow edge virus（SMYEV）， which are found world\|wide， can cause serious crop losses. Total RNA for reverse transcriptase\|polymerase chain reaction （RT\|PCR） was extracted from strawberry with CTAB method. A multiplex RT\|PCR assay was used for simultaneous detecting SMoV and SMYEV based on ndh B gene as the internal control. Both field plants and test\|tube plantlets of strawberries were detected effectively.

Key words: strawberry virus, internal control, multiplex RT\|PCR, virus detection

王红;王菁菁;张科立;成卓敏. 利用内标为基础的多重RT\|PCR技术检测草莓斑驳病毒和草莓轻型黄边病毒[J]. , 2014, 26(1): 0-109.

WANG Hong;WANG Jing\|jing;ZHANG Ke\|li;CHENG Zhuo\|min. Detection of Strawberry mottle virus and Strawberry mild yellow edge virus with multiplex RT\|PCR based on internal control[J]. , 2014, 26(1): 0-109.

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[2]	朱凝瑜，孔蕾，郑天伦. 2013年浙江省南美白对虾虾苗病毒携带情况分析 [J]. 浙江农业学报, 2015, 27(5): 756-.

利用内标为基础的多重RT\|PCR技术检测草莓斑驳病毒和草莓轻型黄边病毒

Detection of Strawberry mottle virus and Strawberry mild yellow edge virus with multiplex RT\|PCR based on internal control

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