关键词: 禽流感病毒, 基质蛋白基因, 环介导等温扩增反应

Abstract: With reference to the matrix protein gene of Avian Influenza Virus(AIV)， we designed two pairs of primers to specifically recognize its six different sites， and established the Loopmediated Isothermal Amplification Reaction (LAMP) method based on the determined color. With different avian viruses tested， the results showed that the method only specifically detected AIV (H9 and H5 subtype) nucleic acids， and the detection limit for AIV was 10 EID50. We also detected the samples for artificial infections and discovered that the coincidence rate of LAMP to the virus isolation was 967%. The experimental data demonstrated that the established methods were specific， sensitive， and simple， which had great potential for clinical application．

Key words: avian influenza virus, matrix protein gene, loopmediated isothermal amplification