黄孢原毛平革菌茶皂素脱色酶的粗分离及酶学性质

浙江农业学报

黄孢原毛平革菌茶皂素脱色酶的粗分离及酶学性质

1浙江省农业科学院植物保护与微生物研究所，浙江杭州 310021；2浙江省植物有害生物防控重点实验室—省部共建国家重点实验室培育基地，浙江杭州 310021；3浙江中医药大学，浙江杭州 310053

出版日期:2014-07-25 发布日期:2014-08-06

Separation and properties of Phanerochaete chrysosporium TS07 tea saponin decoloring enzyme#br#

1 Institute of Plant Protection and Microbiology， Zhejiang Academy of Agricultural Sciences， Hangzhou 310021， China;2 State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control， Hangzhou 310021， China;3 Zhejiang Chinese Medical University， Hangzhou 310053， China

Online:2014-07-25 Published:2014-08-06

摘要/Abstract

摘要： 对黄孢原毛平革菌(Phanerochaete chrysosporium)TS07发酵液中的茶皂素脱色酶进行粗分离，并对其酶学性质进行了研究。结果表明: 用硫酸铵沉降法所得粗酶粉的酶活力高达52 971 U·g-1；该酶的最适反应温度为40℃，最适作用pH值为45；该酶在常温下具有良好的热稳定性，在pH值30～75环境下都比较稳定；Fe3+、Ca2+和Ni2+对酶活影响不大，Hg2+能强烈抑制茶皂素脱色酶的活性；10%(V/V)有机溶剂丙酮、异丙醇、乙醇和乙腈能保留茶皂素脱色酶大部分的活力；反应体系中H2O2达到5%，茶皂素脱色酶仍能保持较高的活力；而在反应底物不存在的情况下，茶皂素脱色酶与025%的H2O2保温1 h后，酶活残余量仍能达到80%以上。

关键词: 黄孢原毛平革菌, 茶皂素脱色酶, 酶学性质

Abstract: A tea saponin decoloring enzyme (TSDE) was separated from Phanerochaete chrysosporium TS07 by using ammonium sulfate precipitation. The separated TSDE activity was 52　971 U·g-1. The effect of pH value on TSDE activity and stability was examined at pH values ranging from 30 to 100. TSDE was completely stable in a wide pH range (30-75) and presented an optimum pH value of 45. The optimum temperature for TSDE was 40℃ and the enzyme was stable at room temperature. It was also found that TSDE was strongly inhibited by Hg2+， while Fe3+, Ca2+ and Ni2+ did not cause any alteration in the activity. TSDE was also resistant to presence of up to 5% H2O2， but it was inhibited in presence of 10% H2O2. The TSDE showed a high percentage of activity in reaction mixtures containing 10% (V/V) of different organic solvents (acetone， isopropanol， ethanol and acetonitrile). The susceptibility of TSDE to inactivation by H2O2 in absence of substrate was also studied and it was found that the enzyme was very stable in presence of up to 025% H2O2．

Key words: Phanerochaete chrysosporium, tea saponin decoloring enzyme, enzyme properties

李艳丽1，2，陈列忠1，2，*，陶瑛妮3. 黄孢原毛平革菌茶皂素脱色酶的粗分离及酶学性质[J]. 浙江农业学报.

LI Yanli1，2， CHEN Liezhong1，2，*， TAO Yingni3. Separation and properties of Phanerochaete chrysosporium TS07 tea saponin decoloring enzyme#br#[J]. .

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