青粗饲料中多种黄曲霉毒素高效液相色谱检测方法

浙江农业学报

青粗饲料中多种黄曲霉毒素高效液相色谱检测方法

(1浙江省农业科学院畜牧兽医研究所，浙江杭州 310021；2浙江省农业科学院农产品质量标准研究所，浙江杭州 310021)

出版日期:2014-09-25 发布日期:2014-10-11

Synchronous determination of various aflatoxins in coarse fodder by HPLC

(1 Institute of Animal Husbandry & Veterinary Science， Zhejiang Academy of Agricultural Sciences， Hangzhou 310021，China;2 Institute of Quality and Standard for Agro\|products， Zhejiang Academy of Agricultural Sciences， Hangzhou 310021， China)

Online:2014-09-25 Published:2014-10-11

摘要/Abstract

摘要： 饲料样品经提取、免疫亲和柱净化，以甲醇水45∶55(V/V)为流动相，进行C18柱分离，经柱后光化学衍生，以荧光检测器定量检测4种黄曲霉毒素AFB1，AFB2，AFG1和AFG2含量。结果表明，4种黄曲霉毒素在35 min内得到良好的分离，除AFG2加标回收率略低外，AFB1，AFB2，AFG1和黄曲霉毒素总量在稻草、羊草和青贮笋壳空白样的加标回收率均在80%以上，平行样相对标准偏差均低于100%；AFB1、AFB2、AFG1、AFG2和黄曲霉毒素总量的检出限分别为04，02，04，02 和12 μg·kg-1。试验所建立的检测方法可实现对青粗饲料中4种黄曲霉毒素的同时提取、净化、分离与检测，方法准确简便，重复性好，灵敏度高，能满足青粗饲料中多种黄曲霉毒素快速检测要求。

关键词: 黄曲霉毒素, 青粗饲料, 高效液相色谱法

Abstract: The present study was conducted to establish a determination method for various aflatoxins in coarse fodder by HPLC. Aflatoxins were synchronously extracted from samples， cleaned up with immunoaffinity column， separated by elution in C18 column and analyzed by fluorescence detector after the photochemical derivation. Four kinds of aflatoxins were separated within 35 min. The recovery rate of added aflatoxin AFB1， AFB2， AFG1 and total aflatoxins from rice straw， Chinese wildrye and bamboo shell silage were all above 800%. But， the recovery rate of AFG2 was relatively low. The relative standard deviations (RSD) for aflatoxins in parallel samples were less than 100%. The limit of detection (LOD) for aflatoxin AFB1， AFB2， AFG1， AFG2 and total aflatoxins were 04，02，04， 02 and 12 μg·kg-1， respectively. Thus， the established determination method could realize simultaneous extraction， clean up， separation， and detection of aflatoxins in coarse fodder. The method is accurate， simple， high sensitive and well repeatable， which is suitable for the rapid determination of aflatoxin in coarse fodder.

Key words: aflatoxin, coarse fodder, HPLC

郑会超1，周育2，黄新1，吉小凤2，吴建良1，蒋永清1，*. 青粗饲料中多种黄曲霉毒素高效液相色谱检测方法 [J]. 浙江农业学报.

ZHENG Hui\|chao1， ZHOU Yu2， HUANG Xin1， JI Xiao\|feng2， WU Jian\|liang1， JIANG Yong\|qing1，*. Synchronous determination of various aflatoxins in coarse fodder by HPLC [J]. .

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