红叶石楠芽变株系组培快繁技术研究

浙江农业学报

红叶石楠芽变株系组培快繁技术研究

（宁波市农业科学研究院，浙江宁波 315040）

出版日期:2014-09-25 发布日期:2014-10-11

Study on rapid propagation of mutant Photinia fraseri in vitro

（Ningbo Academy of Agricultural Sciences，Ningbo 315040， China）

Online:2014-09-25 Published:2014-10-11

摘要/Abstract

摘要： 以红叶石楠芽变带芽茎段为外植体，开展组培快繁技术研究，重点探索6\|BA，NAA，IBA浓度组合对变异株系的增殖、生根及性状稳定性的影响。结果表明：最适宜的增殖培养基为：MS＋05 mg·L-1 NAA+15 mg·L-1 6\|BA，变异性状稳定率达到100%；最适宜的生根培养基为：1/2MS+05 mg·L-1 NAA，30 d生根率达到68%，移栽成活率达90%以上。

关键词: 红叶石楠, 芽变, 组培快繁

Abstract: Taking the mutant shoots of Photinia fraseri as explants， studies were carried out to understand the effects of different concentration combinations of 6\|BA， NAA， IBA on the mutant proliferation， rooting and stability of Photinia fraseri. The results showed that: the optimal proliferation medium was MS+05 mg·L-1 NAA+15 mg·L-1 6\|BA， and the stability rate of mutant characters reached 100%. The most suitable medium for rooting was 1/2MS+05 mg·L-1 NAA， and the rooting rate reached 68% in 30 d， the transplanting survival rate was more than 90%.

Key words: Photinia fraseri, bud mutation, tissue culture and rapid propagation

沈岚，章建红，倪建刚，朱宏芬，刘健，赵天荣，张国芳. 红叶石楠芽变株系组培快繁技术研究 [J]. 浙江农业学报.

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