罗汉果果实总RNA提取方法的比较研究

浙江农业学报

罗汉果果实总RNA提取方法的比较研究

（1 广西药用植物园，广西南宁530023；2 中国医学科学院&北京协和医学院，药用植物研究所，北京100193）

出版日期:2014-11-25 发布日期:2014-12-02

Comparison of methods for total RNA extraction from Siraitia grosvenorii fruits

（1 Guangxi Botanical Garden of Medicinal Plants，Nanning 530023，China；2 Institute of Medicinal Plant Development，Chinese Academy of Medical Sciences & Peking Union Medical College，Beijing 100193，China）

Online:2014-11-25 Published:2014-12-02

摘要/Abstract

摘要： 针对罗汉果果实富含多糖和多酚类物质的特点，以广西罗汉果代表种质青皮果种的果实为材料，比较了改良Trizol法、改良异硫氰酸胍法、改良CTAB法和常规Trizol法4种不同的总RNA提取方法的提取效果。结果表明，改良Trizol法所提取的RNA呈现28S rRNA、18S rRNA和5S rRNA 3条清晰的谱带，且28S条带宽度接近18S的2倍，纯度高（D260/D280=201；D260/D230=202），完整性好（RIN=950），得率为（26000±1947） μg·g-1。RT\|PCR结果进一步表明，改良Trizol法提取的RNA完全能够用于后续的分子生物学研究。

关键词: 罗汉果果实, 总RNA提取, 改良Trizol法

Abstract: Total RNA was isolated from Siraitia grosvenorii fruits of Qingpiguo in Guangxi by the methods of Trizol， improved Trizol， modified guanidine thiocyanate and CTAB， with rich polyphenols and polysaccharide. The results showed that RNA isolated by the improved Trizol showed three clear bands of 28S rRNA， 18S rRNA and 5S rRNA, respectively, and the 28S was about twice in length as 18S， which indicated that little RNA was decomposed and the purity of RNA was very high （D260/D280=201；D260/D230=202）. In addition， the RNA integrity （RIN） and yield rate of RNA extracted by the improved Trizol was 950 and （26000±1947） μg·g-1， respectively. RT\|PCR amplification also showed that the quality of RNA isolated from the improved Trizol can meet the demands of molecular biology experiment.

Key words: Siraitia grosvenorii fruit, total RNA extraction, improved Trizol method

韦荣昌1，2，马小军2，白隆华1，吴庆华1，施力军1，潘丽梅1，冯世鑫1，莫长明1. 罗汉果果实总RNA提取方法的比较研究 [J]. 浙江农业学报.