关键词: 建立肉鸭黄曲霉毒素B1（Aflatoxin B1, 简写AFB1）慢性中毒模型, 设立正常组、模型组、阳性对照组（亚硒酸钠饲喂组）、&ldquo, 复方柴芩颗粒&rdquo, 高、中、低剂量组, 利用高效液相色谱技术检测肉鸭肝、肾组织中AFB1含量以及用肝脏切片、血液生化指标、氧化应激等指标来探讨&ldquo, 复方柴芩颗粒&rdquo, 对治疗鸭慢性中毒药理作用及其机理。结果显示, 同模型组相比, &ldquo, 复方柴芩颗粒&rdquo, 高、中、低剂量组, 肝和肾组织中AFB1含量和血清中丙二醛（MDA）含量、碱性磷酸酶（AKP）、谷丙转氨酶（GPT）、谷草转氨酶（GOT）活力显著降低, 超氧化物歧化酶（SOD）显著升高。投药中剂量短期（7和14 d）, 各项指标更接近正常值。本研究表明, &ldquo, 复方柴芩颗粒&rdquo, 能有效防治肉鸭黄曲霉毒素B1慢性中毒, 减少肝细胞膜受损或细胞坏死程度, 抑制脂质过氧化反应, 上调肝脏抗氧化的能力, 且投药中剂量短期（7或14 d）药效最好。

Abstract: The aflatoxin B1（AFB1） chronic poisoning model of meat ducklings was set up， and the feeding treatments included the normal group， the model group， positive control group （sodium selenite feeding group）， “Compound Chai Qin Particles” groups with high， middle， low doses. Serum， liver and kidney were taken to detect the AFB1 content， blood biochemical and oxidative stress indexes to discuss the pharmacological mechanism of “Compound Chai Qin Particles” in treating duck chronic poisoning of AFB1. Compared with the model group， AFB1 content in liver and kidney decreased significantly in three dose “Compound Chai Qin Particles” groups （P<005）， AKP activity， GOT activity and GPT activity decreased significantly （P<005）， SOD activity increased significantly， but MDA content significantly decreased in serum of the three dose “Compound Chai Qin Particles” groups （P<005）. During 7 or 14 d， the indexes were closer to the normal ones in the middle\|dose of “Compound Chai Qin Particles” group. It was concluded that “Compound Chai Qin Particles” could effectively control ducks chronic poisoning of AFB1， reduce the degree of liver cell membrane damage or cell necrosis， inhibit lipid peroxidation， and increase the antioxidant abilityof liver. And middle\|dose group showed the best effect during the period of 7 d or 14 d after setting up model by AFB1.

Key words:  Compound Chai Qin Particles, aflatoxin B1, high performance liquid chromatography, duck, liver tissue