浙江农业学报

• 生物系统工程 • 上一篇    下一篇

基于Weibull分布的高压脉冲电场杀菌动力学模型

  

  1. (杭州电子科技大学 生命信息与仪器工程学院,浙江 杭州 310018)
  • 出版日期:2015-08-25 发布日期:2015-08-21

Inactivation kinetics model of Escherichia coli by pulsed electric field derived from Weibull model#br#

  1. (College of Life Information Science and Instrument Engineering, Hangzhou Dianzi University, Hangzhou 310018, China)
  • Online:2015-08-25 Published:2015-08-21

摘要: 研究了高压脉冲电场技术对橙汁中大肠杆菌的杀菌动力学模型。以橙汁中普遍存在的大肠杆菌作为研究对象,分析各物理量(电场强度、处理时间、初始温度)及它们的交互作用对大肠杆菌的杀菌效果。首先,采用场发射扫描电子显微镜观察高压脉冲电场对大肠杆菌细胞的破坏作用,然后通过Weibull模型对试验数据进行拟合,得到大肠杆菌的杀菌预测模型,并通过独立试验数据验证模型的偏移因素和准确度因素。结果表明,Weibull模型可用于预测高压脉冲电场技术对橙汁中大肠杆菌的杀菌效果。

关键词: 高压脉冲电场, Weibull分布, 大肠杆菌, 杀菌动力学模型

Abstract: The aims of this study were to study the inactivation kinetic model of Escherichia coli in orange juice by pulsed electric field (PEF) treatment. The lethal effects of various PEF processing parameters (electric field intensity, treatment time, initial temperature) were analyzed to evaluate the efficiency of PEF on the inactivation of Escherichia coli which was common in orange juice. The results from field emission scanning eleciron microscope (FESEM) imaging of Escherichia coli indicated that the PEF treatment induced the destruction of cell membrane structures. The inactivation kinetic model of Escherichia coli was obtained by fitting with Weibull model, and the model was validated by bias factor and accuracy factor. This research suggested that the Weibull model was suitable for predicting the survival ratio of Escherichia coli in orange juice treated by PEF.

Key words: pulsed electric field, Weibull model, Escherichia coli, inactivation kinetic model