八眉猪SLADRA基因第2外显子多态性分析

浙江农业学报

八眉猪SLADRA基因第2外显子多态性分析

（西北民族大学生命科学与工程学院，甘肃兰州730030）

出版日期:2016-06-25 发布日期:2016-06-12

Polymorphism of SLADRA exon 2 in Bamei pigs

（College of Life Science and Engineering， Northwest University for Nationalities， Lanzhou 730030， China）

Online:2016-06-25 Published:2016-06-12

摘要/Abstract

摘要： 为了解八眉猪SLADRA基因第2外显子的多态性，采用PCRSSCP和克隆测序的方法对八眉猪SLADRA基因第2外显子进行分型，并对不同等位基因进行核苷酸与氨基酸序列分析。结果显示，222头八眉猪中共检出2种等位基因（A和B）和3种基因型（AA，BB和AB），其中A等位基因和AA基因型的频率最高，为优势基因和优势基因型。对不同SSCP带型的对应片段进行测序分析，发现该基因仅有一个核苷酸突变位点（c.3093 A→C），为错义突变，使甲硫氨酸（Met）变为亮氨酸（Leu），并且该氨基酸位于抗原结合位点上。群体遗传学分析发现，八眉猪遗传杂合度（He）为0.351 4，多态信息含量（PIC）为0.308 8，且经χ2适合性检验，八眉猪在此位点上没有偏离HardyWeinberg平衡状态（P>0.05）。八眉猪SLADRA基因第2外显子的多态性较低。

关键词: 八眉猪, SLADRA基因, PCRSSCP

Abstract: In order to study polymorphism of SLADRA exon 2 in Bamei pigs， genetic variation of SLADRA exon 2 in Bamei pigs was identified by polymerase chain reactionsingle strand conformation polymorphism and cloning， both the nucleotide and the amino acid sequences of the different alleles were analyzed. The results showed that two alleles （A， B） and three genotypes （AA， BB， AB） of SLADRA exon 2 were detected from 222 Bamei pigs， the frequencies of the A allele and AA genotype were the highest， and A and AA were dominant. The results of sequencing showed that only one mutational nucleotide site （c.3093 A→C） and one amino acid site （p. Met→Leu） were identified. Genetic heterozygosity was 0.351 4， and polymorphic information content （PIC） was 0.308 8， which meaned SLADRA exon 2 in Bamei pigs was moderately polymorphic （0.25 <PIC <0.5）. The chisquare analysis suggested that the allele and genotype frequencies were not deviated from HardyWeinberg equilibrium （P > 0.05）.The finding showed that polymorphism of SLADRA exon 2 in Bamei pigs was low.

Key words: Bamei pigs, SLADRA, PCRSSCP

金丽，谭小音，母童，王国梅，张丽，刘丽霞*. 八眉猪SLADRA基因第2外显子多态性分析[J]. 浙江农业学报.

JIN Li， TAN Xiaoyin， MU Tong， WANG Guomei， ZHANG Li， LIU Lixia*. Polymorphism of SLADRA exon 2 in Bamei pigs[J]. .

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