猪Nramp1基因内含子6多态性与仔猪腹泻的相关性

doi:10.3969/j.issn.1004-1524.2017.06.04

浙江农业学报 ›› 2017, Vol. 29 ›› Issue (6): 882-887.DOI: 10.3969/j.issn.1004-1524.2017.06.04

猪Nramp1基因内含子6多态性与仔猪腹泻的相关性

古少波, 杜倩, 舒丹, 李志瑕, 田晓静, 刘丽霞^*

西北民族大学生命科学与工程学院,甘肃兰州730030

收稿日期:2016-12-19 出版日期:2017-06-20 发布日期:2017-09-07
通讯作者: 刘丽霞,E-mail: skllx@xbmu.edu.cn
作者简介:古少波(1994—),男,山西朔州人,本科生,研究方向为分子生物学。E-mail:gu.1994@foxmail.com
基金资助:
西北民族大学中央学校基本科研业务费专项资金资助项目(31920170029); 西北民族大学本科生科研创新项目(URIP16196)

Polymorphism of Nramp1 intron 6 and its correlation with piglet diarrhea

GU Shaobo, DU Qian, SHU Dan, LI Zhixia, TIAN Xiaojing, LIU Lixia^*

College of Life Science and Engineering,Northwest Minzu University, Lanzhou 730030,China

Received:2016-12-19 Online:2017-06-20 Published:2017-09-07

摘要/Abstract

摘要： 为了研究猪Nramp1基因内含子6的多态性与仔猪腹泻的相关性,采用PCR-RFLP法对八眉猪和烟台黑猪Nramp1基因内含子6的多态性进行研究。结果表明:两个猪种在该基因内含子6的多态性较低,共检测到两个等位基因(A、B)和两种基因型(AB、BB),八眉猪多态信息含量(PIC)为0.33,烟台黑猪为0.36。卡方适合性检验显示,两猪种的Nramp1 基因内含子6极显著偏离Hardy-Weinberg平衡(P<0.01)。相关性分析结果表明,八眉猪对腹泻的抵抗能力显著高于烟台黑猪(P<0.05)。性别与Nramp1基因内含子6基因型对仔猪腹泻影响不显著(P>0.05),而性别与基因型的互作对仔猪腹泻有显著影响(P<0.05),在母猪群体中,AB基因型的腹泻评分极显著大于BB基因型(P<0.01);在公猪群体中,AB和BB基因型腹泻评分差异不显著(P>0.05)。

关键词: 八眉猪, 烟台黑猪, Nramp1基因, 腹泻, 多态性

Abstract: In order to research the polymorphism of Nramp1 intron 6 and its correlation with piglet diarrhea, this study analyzed the polymorphism of the Nramp1 intron 6 in Bamei pig and Yantai black pig by PCR-RFLP. The results showed that the polymorphisms of Nramp1 intron 6 were not rich in two pig breeds, two alleles (A, B) and two genotypes (AB, BB) were detected, the polymorphic information content was 0.33 in Bamei pig and 0.36 in Yantai black pig. The Chi square fit test showed that the Nramp1 intron 6 deviated from Hardy-Weinberg balance extremely in two pig breeds (P<0.01). The results of the least square showed that, the diarrhea resistance ability of Bamei pig was stronger than that of Yantai black pig (P<0.05). The gender and genotype of the Nramp1 intron 6 did not significantly influence piglet diarrhea alone (P>0.05), but the interaction of the gender and genotype influenced piglet diarrhea significantly (P<0.05) in female piglets, and the diarrhea score of genotype AB was greater than that of genotype BB (P<0.01), while in male piglets, these two genotypes had not significant difference in diarrhea score (P>0.05).

Key words: Bamei pig, Yantai black pig, Nramp1 gene, diarrhea, polymorphism

中图分类号:

S828

古少波, 杜倩, 舒丹, 李志瑕, 田晓静, 刘丽霞. 猪Nramp1基因内含子6多态性与仔猪腹泻的相关性[J]. 浙江农业学报, 2017, 29(6): 882-887.

GU Shaobo, DU Qian, SHU Dan, LI Zhixia, TIAN Xiaojing, LIU Lixia. Polymorphism of Nramp1 intron 6 and its correlation with piglet diarrhea[J]. , 2017, 29(6): 882-887.

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猪Nramp1基因内含子6多态性与仔猪腹泻的相关性

Polymorphism of Nramp1 intron 6 and its correlation with piglet diarrhea

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