蛋用鹌鹑IGF-1R基因的多态性与体尺性状相关性分析

doi:10.3969/j.issn.1004-1524.2020.03.03

浙江农业学报 ›› 2020, Vol. 32 ›› Issue (3): 398-405.DOI: 10.3969/j.issn.1004-1524.2020.03.03

蛋用鹌鹑IGF-1R基因的多态性与体尺性状相关性分析

白俊艳^1,2, 卢军浩³, 付学言¹, 武晓红¹, 杨又兵^1,2, 雷莹^1,2, 庞有志^1,2,*, 卢小宁¹, 巩慧荣¹, 胡陆星¹, 刘红涛¹, 樊红灯¹, 曹恒¹, 时坤鹏¹, 陈梦柯¹, 马永康¹

1.河南科技大学动物科技学院,河南洛阳 471023;
2.洛阳市动物遗传育种重点实验室,河南洛阳 471023;
3.甘肃农业大学动物科学技术学院,甘肃兰州 730070

收稿日期:2019-06-15 出版日期:2020-03-25 发布日期:2020-04-03
通讯作者: *庞有志,E-mail: pyz2006@126.com
作者简介:白俊艳(1975—),女,内蒙古赤峰人,博士,副教授,研究方向为动物分子遗传育种。E-mail:junyanbai@163.com
基金资助:
国家自然科学基金(31201777); 河南省产学研合作项目(152107000095.0)

Analysis of correlation between polymorphism of IGF-1R gene and body size traits in egg quail

BAI Junyan^1,2, LU Junhao³, FU Xueyan¹, WU Xiaohong¹, YANG Youbing^1,2, LEI Ying^1,2, PANG Youzhi^1,2,*, LU Xiaoning¹, GONG Huirong¹, HU Luxing¹, LIU Hongtao¹, FAN Hongdeng¹, CAO Heng¹, SHI Kunpeng¹, CHEN Mengke¹, MA Yongkang¹

1.College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471023, China;
2.Luoyang Key Laboratory of Animal Genetics and Breeding, Luoyang 471023, China;
3.College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China

Received:2019-06-15 Online:2020-03-25 Published:2020-04-03

摘要/Abstract

摘要： 类胰岛素生长因子1型受体(IGF-1R)基因是类胰岛素生长因子(IGFs)在生物体内发挥作用的主要效应器,对机体生长发育进程中有重要影响。为探讨IGF-1R基因多态性与蛋用鹌鹑体尺性状的相关性,采用PCR产物直接测序方法对中国黄羽鹌鹑、北京白羽鹌鹑、朝鲜鹌鹑3种蛋用鹌鹑的IGF-1R基因进行多态性分析,并对IGF-1R基因的多态性与鹌鹑体尺性状进行相关性分析。结果表明,在3个蛋用鹌鹑群体中检测到IGF-1R基因A57G和A72T两个突变位点,A57G位点发生了A/G碱基突变,有AA、AG和GG三种基因型;A72T位点发生了A/T碱基突变,有AA、AT和TT三种基因型。中国黄羽鹌鹑的A57G位点对胫长、胸骨长和胫围有显著影响(P<0.05),A72T位点对体质量和胸骨长有显著影响(P<0.05),组合基因型AGAA对体质量有显著影响(P<0.05),组合基因型AAAA对胫长、胸骨长、胫围有明显影响(P<0.05)。北京白羽鹌鹑的A57G位点对体质量、胫长、体长、胫围有显著影响(P<0.05),A72T位点对胸骨长有显著影响(P<0.05),组合基因型AAAA对体质量和胸骨长有显著影响(P<0.05),组合基因型AAAT、GGAA、GGAT对胫长有显著影响(P<0.05),组合基因型GGAA对胸深有显著影响(P<0.05)。朝鲜鹌鹑的A57G位点对体质量有显著影响(P<0.05),A72T位点对胸骨长和胫围有显著影响(P<0.05),组合基因型AGAA对体质量有显著影响(P<0.05),组合基因型AGAT对胫长和胸宽有显著影响(P<0.05)。因此,IGF-1R基因可以作为候选基因应用于蛋用鹌鹑体尺性状的分子标记辅助选择。

关键词: 蛋用鹌鹑, 类胰岛素生长因子1型受体(IGF-1R)基因, 多态性, 体尺性状, 相关性分析

Abstract: Type 1 insulin-like growth factor receptor (IGF-1R) gene is the main effector of insulin-like growth factor (IGFs). It has important influence on the growth and development of the body. To investigate the correlation within IGF-1R and body size traits of egg quails, the polymorphism of IGF-1R gene was analyzed by direct sequencing of PCR products in Chinese yellow quail, Beijing white quail and Korean quail, and the correlation within the polymorphism of IGF-1R gene and body size traits of quail was analyzed. The results showed that two mutation sites of IGF-1R gene A57G and A72T were detected in three egg quail populations. A/G base mutation occurred at A57G site, including three genotypes of AA, AG and GG. A/T base mutation occurred at A72T site, including three genotypes of AA, AT and TT. The A57G locus of Chinese yellow quail had significant (P<0.05)effects on tibia length, sternal length and tibia circumference, A72T locus had significant (P<0.05) effects on body weight and sternal length, combination genotype AGAA had significant (P<0.05) effects on body weight, combination genotype AAAA had significant effects on tibia length, sternal length and tibia circumference (P<0.05). The A57G locus of Beijing white quail had significant effects on body weight, tibia length, body length and tibia circumference (P<0.05), A72T locus had significant (P<0.05) effect on sternal length, combination genotypes AAAA had significant (P<0.05) effects on body weight and sternal length, and combination genotypes AAAT, GGAA and GGAT had significant (P<0.05) effects on tibia length, the combined genotype GGAA had significant (P<0.05) effect on chest depth. The A57G locus of Korean quail had significant (P<0.05) effect on body weight, and the A72T locus had significant (P<0.05) effect on sternal length and tibia circumference, the combined genotype AGAA had significant (P<0.05) effect on body weight, and the combined genotype AGAT had significant (P<0.05) effect on tibia length and chest width. Therefore, the IGF-1R gene could be used as a candidate gene for molecular marker-assisted selection of body size traits in egg quails.

Key words: egg quail, type 1 insulin-like growth factor receptor (IGF-1R) gene, polymorphism, body size traits, correlation analysis

中图分类号:

S839

白俊艳, 卢军浩, 付学言, 武晓红, 杨又兵, 雷莹, 庞有志, 卢小宁, 巩慧荣, 胡陆星, 刘红涛, 樊红灯, 曹恒, 时坤鹏, 陈梦柯, 马永康. 蛋用鹌鹑IGF-1R基因的多态性与体尺性状相关性分析[J]. 浙江农业学报, 2020, 32(3): 398-405.

BAI Junyan, LU Junhao, FU Xueyan, WU Xiaohong, YANG Youbing, LEI Ying, PANG Youzhi, LU Xiaoning, GONG Huirong, HU Luxing, LIU Hongtao, FAN Hongdeng, CAO Heng, SHI Kunpeng, CHEN Mengke, MA Yongkang. Analysis of correlation between polymorphism of IGF-1R gene and body size traits in egg quail[J]. , 2020, 32(3): 398-405.

参考文献

[1] 金崇富, 陈长宽, 杨智青, 等. 鸡IGF1R、IGFBP-3基因SNP位点之间互作效应分析[J]. 江苏农业学报, 2015, 31(5): 1099-1104.
JIN C F, CHEN C K, YANG Z Q, et al.Genetic interaction effect between SNPs loci of IGF1R and IGFBP-3 genes in chicken[J]. Jiangsu Journal of Agricultural Sciences, 2015, 31(5): 1099-1104.(in Chinese with English abstract)
[2] 赵秀华, 王金玉, 张跟喜, 等. IGF-I与IGFBP-1基因对京海黄鸡生长性状的遗传效应分析[J]. 畜牧兽医学报, 2012, 43(1): 152-158.
ZHAO X H, WANG J Y, ZHANG G X, et al.Genetic effects of IGF-I and IGFBP-1 genes on the growth traits in Jinghai yellow chicken[J]. Chinese Journal of Animal and Veterinary Sciences, 2012, 43(1): 152-158.(in Chinese with English abstract)
[3] 吕莉, 宋陈玲, 寇洁, 等. IGF1R和IGF2R基因在鸭脂肪组织中的发育表达模式比较[J]. 中国畜牧杂志, 2013, 49(1): 5-10.
LYU L, SONG C L, KOU J, et al.Comparison between developmental expression pattern of IGF1R and IGF2R gene in duck adipose tissue[J]. Chinese Journal of Animal Science, 2013, 49(1): 5-10.(in Chinese with English abstract)
[4] SZEWCZUK M.Analysis of the relationship between insulin-like growth factor 1 receptor gene polymorphisms in Montbeliarde cows and the birth weight of their calves[J]. Acta Veterinaria Brno, 2016, 85(1): 41-47.
[5] GUO L, YANG S, LI M M, et al.Divergence and polymorphism analysis of IGF1Ra and IGF1Rb from orange-spotted grouper, Epinephelus coioides(Hamilton)[J]. Genetics and Molecular Research, 2016, 15(4): 1-12.
[6] 赵飘,何敏,吕莹莹,等. 中国黄羽鹌鹑哈德氏腺发育的增龄变化及形态组织学观察[J]. 浙江农业学报,2019,31(4):525-531.
ZHAO P, HE M, LYU Y Y, et al.Age-related histomorphological studies on Harderian gland of Chinese yellow quail[J]. Acta Agriculturae Zhejiangensis, 2019, 31(4): 525-531. (in Chinese with English abstract)
[7] 潘爱銮, 皮劲松, 杜金平, 等. 中国鹌鹑遗传研究进展[J]. 湖北农业科学, 2012, 51(23): 5263-5266.
PAN A L, PI J S, DU J P, et al.Progress of quail genetic research in China[J]. Hubei Agricultural Sciences, 2012, 51(23): 5263-5266.(in Chinese with English abstract)
[8] 墨锋涛. 鹌鹑FMO₃基因克隆及其多态性与蛋黄三甲胺含量的关联分析[D]. 北京: 中国农业大学, 2014.
MO F T.Cloning of quail FMO₃ gene and association of gene polymorphisms with trimethylamine concentration in egg yolk[D]. Beijing: China Agricultural University, 2014.(in Chinese with English abstract)
[9] 吴胜军, 庞有志. 鹌鹑遗传多样性研究进展[J]. 家禽科学, 2016(3): 47-52.
WU S J, PANG Y Z.Progress in the study of genetic diversity of quail[J]. Poultry Science, 2016(3): 47-52.(in Chinese with English abstract)
[10] 杨帅,白俊艳,庞有志,等. 三个品种母鹌鹑的体重和体尺性状比较分析[J]. 黑龙江畜牧兽医,2019(3):145-148.
YANG S, BAI J Y, PANG Y Z, et al.Comparative analysis of body weight and size traits of three female quails[J]. Heilongjiang Animal Science and Veterinary Medicine, 2019(3):145-148.(in Chinese)
[11] 吴鹏飞, 王冬, 张向前, 等. 边鸡IGF-ⅠR基因AluⅠ位点多态性及其对生长性状和繁殖性状的遗传效应分析[J]. 中国畜牧兽医, 2017, 44(3): 813-818.
WU P F, WANG D, ZHANG X Q, et al.Study on the polymorphisms of IGF-ⅠR gene AluⅠ locus and genetic effect on growth and reproduction traits in Bian chicken[J]. China Animal Husbandry & Veterinary Medicine, 2017, 44(3): 813-818.(in Chinese with English abstract)
[12] EL-MAGD M A, SALEH A A, NAFEAA A A, et al. Polymorphisms of the IGF1 gene and their association with growth traits, serum concentration and expression rate of IGF1 and IGF1R in buffalo[J]. Journal of Zhejiang University-SCIENCE B, 2017, 18(12): 1064-1074.
[13] RAMESHA K P, RAO A, BASAVARAJU M, et al.Genetic variability of bovine GHR, IGF-1 and IGFBP-3 genes in Indian cattle and buffalo[J]. South African Journal of Animal Science, 2015, 45(5):485-493.
[14] CHENG Y Y, LIU S C, ZHANG X, et al.Expression profiles of IGF-1R gene and polymorphisms of its regulatory regions in different pig breeds[J]. The Protein Journal, 2016, 35(3): 231-236.
[15] 李伟, 张飞燕, 宋婷婷, 等. 藏猪与雅南猪IGF-I基因的克隆与序列分析[J]. 浙江农业学报, 2015, 27(8): 1324-1330.
LI W, ZHANG F Y, SONG T T, et al.Cloning and sequence analysis of IGF-I gene in Tibetan pig and Yanan pig[J]. Acta Agriculturae Zhejiangensis, 2015, 27(8): 1324-1330.(in Chinese with English abstract)
[16] 陈则东, 王文浩. 苏禽黄鸡IGF1R基因第16外显子多态性及其与生长性状的关联研究[J]. 现代农业科技, 2017(16): 224-225.
CHEN Z D, WANG W H.Study on correlation between polymorphism of IGRF1R gene exon 16 and production performance in Suqin yellow chicken[J]. Modern Agricultural Science and Technology, 2017(16): 224-225.(in Chinese with English abstract)
[17] YANG F P, JIN C F, DAI G J, et al.Polymorphisms in exon 2 of IGF-IR gene and theirs association with production traits in Jinghai yellow chicken[J]. Journal of Animal and Veterinary Advances, 2012, 11(19): 3499-3505.
[18] MOE H H, SHIMOGIRI T, KAMIHIRAGUMA W, et al.Analysis of polymorphisms in the insulin-like growth factor 1 receptor (IGF1R) gene from Japanese quail selected for body weight[J]. Animal Genetics, 2007, 38(6): 659-661.
[19] 高凤华, 卞立红, 王守志, 等. 鸡IGF1R基因多态性与生长和体组成性状的相关性研究[J]. 东北农业大学学报, 2009, 40(1): 77-83.
GAO F H, BIAN L H, WANG S Z, et al.Association of chicken IGF1R gene with growth and body composition traits[J]. Journal of Northeast Agricultural University, 2009, 40(1): 77-83.(in Chinese with English abstract)
[20] 杨凤萍, 金崇富, 俞亚波, 等. 京海黄鸡IGF-IR基因外显子3多态性及遗传效应分析[J]. 畜牧与兽医, 2013, 45(7): 1-5.
YANG F P, JIN C F, YU Y B, et al.Polymorphisms of IGF-IR gene exon3 and theirs genetic effect analysis in Jinghai yellow chickens[J]. Animal Husbandry & Veterinary Medicine, 2013, 45(7): 1-5.(in Chinese with English abstract)
[21] 武艳平, 霍俊宏, 谢明贵, 等. 泰和丝羽乌骨鸡IGF-1基因与体重的关联分析[J]. 华北农学报, 2010, 25(S2): 22-26.
WU Y P, HUO J H, XIE M G, et al.Associations between the insulin-like growth factor-1(IGF-1) gene polymorphism and body weight in Taihe silky fowl[J]. Acta Agriculturae Boreali-Sinica, 2010, 25(S2): 22-26.(in Chinese with English abstract)
[22] 肖超能, 翁吉梅, 李维, 等. 家兔IGF1基因多态性与生长性状的关联分析[J]. 中国畜牧兽医, 2017, 44(8): 2378-2385.
XIAO C N, WENG J M, LI W, et al.Association analysis between polymorphism of IGF1 gene and growth traits in rabbit[J]. China Animal Husbandry & Veterinary Medicine, 2017, 44(8): 2378-2385.(in Chinese with English abstract)
[23] NAICY T, VENKATACHALAPATHY R T, ARAVINDAKSHAN T V, et al.Association of a novel single nucleotide polymorphism at the exon-2 of insulin-like growth factor 1 (IGF₁) gene with phenotypic variants in goats[J]. Veterinarski Arhiv, 2017, 87(4): 457-472.
[24] YANG C, ZHANG J B, AHMAD A, et al.Dietary energy levels affect growth performance through growth hormone and insulin-like growth factor 1 in yak (Bos grunniens)[J]. Animals, 2019, 9(2): 39.
[25] MA Y L, WEN Y F, CAO X K, et al.Copy number variation (CNV) in the IGF1R gene across four cattle breeds and its association with economic traits[J]. Archives Animal Breeding, 2019, 62(1): 171-179.
[26] 姬改革, 陶志云, 朱春红, 等. 不同品种鸭胚胎期骨骼肌成肌细胞GHR和IGF-1 mRNA表达差异分析[J]. 浙江农业学报, 2016, 28(3): 406-411.
JI G G, TAO Z Y, ZHU C H, et al.The expression analysis of GHR and IGF-1 mRNA in embryonic skeletal muscle myoblasts of different duck breeds[J]. Acta Agriculturae Zhejiangensis, 2016, 28(3): 406-411.(in Chinese with English abstract)
[27] 沈留红, 江涛, 肖劲邦, 等. 奶牛静脉血和脐静脉血脂联素、瘦素及IGF-1表达水平与犊牛初生体质量相关性研究[J]. 浙江农业学报, 2017, 29(1): 37-43.
SHEN L H, JIANG T, XIAO J B, et al.Correlation between calf birth weights and expression levels of adiponectin, leptin, IGF-1 in cow's venous blood and cord venous blood[J]. Acta Agriculturae Zhejiangensis, 2017, 29(1): 37-43.(in Chinese with English abstract)

蛋用鹌鹑IGF-1R基因的多态性与体尺性状相关性分析

Analysis of correlation between polymorphism of IGF-1R gene and body size traits in egg quail

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	刘新雨, 田洁. 大蒜转录组简单重复序列标记分析与分子标记开发[J]. 浙江农业学报, 2020, 32(9): 1615-1625.
[2]	喻宗岗, 蒋隽, 姚亚铃, 郭英, 李闯, 燕海峰. 雪峰乌骨鸡HSP70基因多态性与精液品质关联分析[J]. 浙江农业学报, 2020, 32(8): 1378-1384.
[3]	沈迪, 陈龙正, 路晓华, 陶建平, 冯顾城, 刘洁霞, 冯凯, 尹莲, 丁旭, 贾丽丽, 徐志胜, 刘惠吉, 熊爱生. 苏南地区29个秋冬茬芹菜品种资源评价[J]. 浙江农业学报, 2020, 32(4): 653-660.
[4]	陈琳, 周青青, 顾青, 郦萍. 实时定量PCR法快速检测水产品中的副溶血性弧菌[J]. 浙江农业学报, 2019, 31(5): 823-828.
[5]	乔中全, 王晓明, 李永欣, 曾慧杰, 蔡能, 刘思思, 陈艺. 38个紫薇品种亲缘关系的ISSR分析[J]. 浙江农业学报, 2019, 31(4): 565-571.
[6]	张娟, 母童, 赵平, 陈佳萍, 冯小芳, 郭鹏, 武泽文, 刘丽元, 蒋秋斐, 顾亚玲. 静原鸡ELOVL5基因遗传多样性研究[J]. 浙江农业学报, 2019, 31(2): 200-206.
[7]	杨又兵, 卞军平, 吴燕, 娄然, 李世豪. 猪FTO基因多态性与肉质性状的关联分析[J]. 浙江农业学报, 2019, 31(12): 1971-1978.
[8]	辛世杰, 王晓慧, 戴国俊, 安婷婷, 张涛, 张跟喜, 谢恺舟, 王金玉, 王宏胜. 京海黄鸡柔嫩艾美尔球虫感染对脾脏和盲肠IL-6、IL-8、CCLi2基因表达量的影响及其相关性[J]. 浙江农业学报, 2019, 31(1): 39-46.
[9]	任卫合, 任稳稳, 马博妍, 高成宏, 李宏旭, 刘丽霞, 曹忻, 张丽. 大耳白兔MSTN基因SNPs位点筛查及生物信息学分析[J]. 浙江农业学报, 2018, 30(12): 2018-2023.
[10]	尹明华, 占学林, 徐文慧, 谢妮妮, 蔡红, 陈荣华. 三叶青种质资源遗传多样性的随机扩增多态性DNA分析[J]. 浙江农业学报, 2018, 30(11): 1839-1848.
[11]	程远, 万红建, 刘超超, 姚祝平, 叶青静, 李志邈, 王荣青, 周国治, 杨悦俭, 陈德梁, 阮美颖. 十六个樱桃番茄品种果实风味品质相关指标比较分析[J]. 浙江农业学报, 2018, 30(11): 1859-1869.
[12]	古少波, 杜倩, 舒丹, 李志瑕, 田晓静, 刘丽霞. 猪Nramp1基因内含子6多态性与仔猪腹泻的相关性[J]. 浙江农业学报, 2017, 29(6): 882-887.
[13]	王道泽, 洪文英, 吴燕君, 陈瑞, 胡选祥. 水稻主栽品种和气象因素对稻瘟病田间流行的影响[J]. 浙江农业学报, 2017, 29(5): 791-798.
[14]	张丽, 刘丽霞, 陈红, 李强子, 刘吴鑫. 中国荷斯坦奶牛Nramp1基因多态性与泌乳性状及产奶量的关联性分析[J]. 浙江农业学报, 2017, 29(3): 389-394.
[15]	李琦, 刘双. 影响苏尼特放牧羊体质量变化的相关因素分析[J]. 浙江农业学报, 2017, 29(10): 1630-1636.