大耳白兔MSTN基因SNPs位点筛查及生物信息学分析

doi:10.3969/j.issn.1004-1524.2018.12.05

浙江农业学报 ›› 2018, Vol. 30 ›› Issue (12): 2018-2023.DOI: 10.3969/j.issn.1004-1524.2018.12.05

大耳白兔MSTN基因SNPs位点筛查及生物信息学分析

任卫合, 任稳稳, 马博妍, 高成宏, 李宏旭, 刘丽霞, 曹忻, 张丽^*

西北民族大学生命科学与工程学院,甘肃兰州 730124

收稿日期:2018-03-09 出版日期:2018-12-25 发布日期:2018-12-28
通讯作者: 张丽,E-mail: zhangli2008@aliyun.com
作者简介:任卫合(1997—),男,陕西渭南人,生物工程专业本科生。E-mail: 962980165@qq.com
基金资助:
西北民族大学引进人才科研项目(xbmuyjrc201316);西北民族大学中央高校基本科研业务费专项资金项目(31920170025);西北民族大学本科教学建设项目(2017XJJG-11)

SNP screening and bioinformatics analysis of MSTN in Japanese White Rabbit

REN Weihe, REN Wenwen, MA Boyan, GAO Chenghong, LI Hongxu, LIU Lixia, CAO Xin, ZHANG Li^*

School of Life Sciences and Engineering, Northwestern University of Nationalities, Lanzhou 730124, China

Received:2018-03-09 Online:2018-12-25 Published:2018-12-28

摘要/Abstract

摘要： 为探明大耳白兔MSTN基因与生长发育及肉质性状的关联性,以大耳白兔为试验对象,构建DNA混合池,采用PCR扩增和直接测序技术对该基因进行单核苷酸多态性(SNP)筛查,从而筛选出对大耳白兔肉质性状有显著影响的SNP位点,利用生物信息学方法从分子水平对大耳白兔MSTN基因编码的蛋白质进行功能预测和同源性分析。结果显示:大耳白兔MSTN基因编码区序列全长1 128 bp,编码375个氨基酸,是一种不稳定的水溶性蛋白;蛋白质分子量42.8 ku,理论等电点为6.97;蛋白质二、三级结构均为混合型,无规则卷曲所占比例最高。在扩增区域的非编码区发现1个SNP位点,该突变不影响编码氨基酸的改变。同源性分析表明,大耳白兔MSTN基因与普通家兔相似度为100%,与家猫相似度较高,亲缘关系很近。

关键词: 大耳白兔, MSTN基因, 单核苷酸多态性, 生物信息学

Abstract: To investigate the relationship within MSTN gene and growth and meat quality traits in Japanese White Rabbit, a mixed DNA pool was built, PCR amplification and direct sequencing techniques were used to screen single nucleotide polymorphism (SNP) of this gene, and the SNP loci with significant effect on the meat quality of Japanese White Rabbit were screened. Functional prediction and homology analysis of proteins encoded by MSTN gene of Japanese White Rabbit were carried out at molecular level by bioinformatics method. The results showed that, the sequence length of MSTN gene coding region of Japanese White Rabbit was 1 128 bp, encoding 375 amino acids, which was an unstable water-soluble protein. The protein molecular weight was 42.8 ku, theoretical equal electric point was 6.97, protein tertiary structure was mixed type, and the irregular curl occupied the highest proportion. One SNP site was found in the non coding region of the amplification area, which did not affect the change of encoded amino acids. Homology analysis showed that, the similarity between MSTN gene of Japanese White Rabbit and common rabbit was 100%, and MSTN gene of Japanese White Rabbit had a higher similarity to the home cat and the kinship was close.

Key words: Japanese White Rabbit, MSTN gene, single nucleotide polymorphism, bioinformatics

中图分类号:

S823.8

任卫合, 任稳稳, 马博妍, 高成宏, 李宏旭, 刘丽霞, 曹忻, 张丽. 大耳白兔MSTN基因SNPs位点筛查及生物信息学分析[J]. 浙江农业学报, 2018, 30(12): 2018-2023.

REN Weihe, REN Wenwen, MA Boyan, GAO Chenghong, LI Hongxu, LIU Lixia, CAO Xin, ZHANG Li. SNP screening and bioinformatics analysis of MSTN in Japanese White Rabbit[J]. , 2018, 30(12): 2018-2023.

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大耳白兔MSTN基因SNPs位点筛查及生物信息学分析

SNP screening and bioinformatics analysis of MSTN in Japanese White Rabbit

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